R90251

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: R90251

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1 whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
rosette leaf (PO:0000014)
  • epinastic (PATO:0000945)
    (Rosette leaf::Shape::epinastic)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: R90251

Determination of introduced cDNA(s)

  • J013084G19 - Rice Full-length cDNAs - Original: J013084G19

    full reading [+] show sequence

    Nucleotide sequence: J013084G19 - full reading
    GACACACAGC AGGTAGCTAG CTTAGCCCCT CACTTTGTGG CAGATCATAC 
    TCGCTGGCTA CACCACCACC CACATCCATC TGAGCAGTGG AATCATCATC
    TCCTCCTATA TCAGTTGTCA TCACCCGGCC TGTAGTGTGC GCTCTGATCC
    TAGTTAGCTG CTGCTCTTCG TTCCGATCCT AGCTCGTTGC GAATTGAGCC
    AACGGATCAA TCGCGCGCTC CGGCCGCCAC AGCCTTTATT ACCCAAGCTG
    TTTCTTTTCC AGAATTTAAA GAAGTAGCAG CTGTAGACAG TAGTTATCCT
    CGTCGCGACC CCTTGTGTGC AGAGCAGGCG GCGGCGGCGG CGTTAGCTAG
    CTCGTCGAGC AACATATATG GCAGCTAGTA GTAGCACGTA GTTACTGCAG
    TGAGGAATTC GTCCGGAATC GCGTTGGGAG CGCGCGGAGT AGCGGACGGC
    TGAGCGGAGC GCTCTCGGTT TGATAAGGGT TCGCGTGTGT GTGTTCGGTG
    TGGGGGAGAG TAATTGCACG CATCACAAGA ACACTCCTCG CCTCTACTTG
    GGCTCTCCTG CTCACGTGAT TCATACTCTC CCTACTCCCC TCTCCCCACA
    TCCGTTGCGC CTTGCATCTA CTCTGTGCCA ATCACCGGGC ATATATACAT
    GGTTGTGCCT GTGCCGACGA CGTTTGAGGG AGCGAGAGAG ATGGAGGTGG
    TGGAGGTGCG TGAGTACAGG GAGGACCGGG ACCGCGCCGC CGTCGAGGAG
    GTGGAGCGGG AGTGCGAGGT GGGCTCCTCC GGCGGCGGCG AAGCCAAGAT
    GTGCCTGTTC ACGGATCTCC TCGGCGACCC GCTCTGCCGC ATTCGCAACT
    CGCCGGCCTA CCTCATGCTG GTAGCGGAGA CAGCGAACGG CGGCGGCGGC
    GGCAATGGCA GGGAGATCAT CGGCCTCATC CGCGGTTGCG TCAAGACCGT
    CGTCTCCGGC GGCAGCGTCC AGGCCGGCAA GGACCCCATC TACTCCAAGG
    TCGCCTACAT CCTCGGCCTT CGCGTCTCGC CTCGTTACCG GAGGAAGGGG
    GTGGGGAAGA AGCTGGTGGG CAGGATGGAG GAGTGGTTCC GGCAGAGCGG
    GGCGGAGTAC TCGTACATGG CGACGGAGCA GGACAACGAG GCGTCGGTGC
    GCCTCTTCAC CGGCCGCTGC GGCTACTCCA AGTTCCGGAC GCCGTCGGTG
    CTCGTGCACC CGGCTCAAGA TGAAGAGCTC CAGCAGGAAG GCGCCATCCC
    CGCAGGCGCA GCAGTCTGCG AAGAGGGTGA GGAAGAGGCT GGACAGGACC
    AAGAGCAGCG CCGCCGTGGC GCTCAAAGGA TTGCAGTTTG TGACTGCAAA
    GGTTGGCAAT GACGGCTGGG CCGCGGTGGA GAAGCGGTTC AATCAGCTGC
    AGGTGGATGG TGTGCTGCTC CGTTCAAGAT TTGGGAAATG CATTGGAATG
    GATGGGTCCG ACGAGTTTGC GGTGCAAATG TTCGATTCTC TGGCGAGGAA
    GAGAGGGATA GTGAAGCAGG TGCTCACTAA GGACGAGCTC AAAGATTTCT
    ATGAGCAATT GACTGATCAG GGGTTTGACA ATCGTCTTCG GACATTCTTT
    GACATGGTTG ACAAGAACGC TGATGGAAGG CTCACAGCAG AAGAGGTTAA
    GGAGATTATT GCCCTTAGTG CATCAGCAAA CAAACTTTCC AAGATCAAGG
    AGCGAGCTGA TGAGTACACA GCACTCATTA TGGAAGAGCT TGACCCTACA
    AACTTGGGAT ACATCGAGAT GGAGGACTTG GAAGCACTAT TGCTTCAGTC
    ACCATCTGAA GCTGCTGCAA GATCAACAAC GACGCACAGC TCCAAACTTA
    GCAAAGCTCT TAGCATGAAG CTTGCGTCTA ACAAAGAAAT GAGCCCAGTT
    CGTCATTACT GGCAGCAGTT CATGTACTTC CTTGAAGAGA ATTGGAAGCG
    CAGTTGGGTT ATGACTCTGT GGATCTCAAT CTGCATTGCC CTTTTCATTT
    GGAAGTTCAT TCAGTACCGT AATCGAGCCG TATTCGGCAT CATGGGTTAT
    TGTGTGACCA CTGCAAAGGG TGCTGCAGAG ACCCTCAAAT TCAACATGGC
    TTTGGTCCTA CTTCCTGTCT GCAGAAATAC AATCACATGG ATTCGGTCAA
    AGACACAGGT TGGAGCTGTT GTACCCTTCA ACGACAATAT AAACTTTCAT
    AAGGTCATAG CCGCAGGTGT TGCAGTTGGT GTTGCTTTGC ATGCAGGTGC
    TCATCTGACA TGTGATTTTC CCCGGCTGCT CCATGCGAGT GATGCACAAT
    ATGAACTAAT GAAGCCCTTC TTTGGGGAGA AGAGGCCACC AAATTACTGG
    TGGTTTGTAA AGGGAACTGA AGGCTGGACA GGTGTGGTCA TGGTGGTGCT
    CATGGCAATA GCATTTACAT TAGCCCAACC ATGGTTCCGA CGTAACAAGC
    TCAAGGACTC CAATCCCCTC AAAAAAAATG ACTGGCTTCA ATGCCTTCTG
    GTTTACCCAC CACCTGTTTG TCATTGTGTA CACTTTGCTC TTTGTCCATG
    GAACGTGCTT GTATCTAAGC AGGAAATGGT ACAAGAAGAC GACATGGATG
    TACCTCGCTG TTCCTGTTGT CCTGTATGTA AGTGAGCGTA TTCTTCGGTT
    GTTTAGGAGC CATGATGCAG TTGGGATTCA GAAGGTTGCA GTGTATCCCG
    GGAATGTATT GGCTCTTTAT ATGTCGAAGC CACCTGGTTT CAGATACCGT
    AGTGGGCAGT ACATCTTCAT AAAATGCACT GCTGTGTCTC CATATGAATG
    GCATCCATTT TCCATAACAT CAGCACCTGG AGATGATTAT CTTAGTGTTC
    ATATTCGCAC AAGGGGTGAT TGGACTTCAC GGCTTAGAAC TGTTTTCTCT
    GAGGCATGCC GACCCCCCAC TGAGGGAGAA AGTGGACTAC TTAGAGCTGA
    CCTTTCCAAG GGAATAACGG ACGAAAAAGC AAGATTCCCA AAACTTTTGG
    TCGATGGACC GTATGGTGCA CCGGCACAAG ATTACCGTGA ATACGATGTG
    CTACTTCTCA TCGGGCTGGG CATCGGAGCC ACCCCTTTGA TTAGCATTGT
    GAAGGACGTG CTTAACCACA TTCAAGGTGA GGGATCAGTT GGAACCACGG
    AGCCGGAGAG CAGCAGCAAG GCGAAGAAGA AACCTTTCAT GACGAAGAGA
    GCCTACTTCT ACTGGGTGAC GAGAGAGGAG GGCTCGTTTG AGTGGTTCAG
    AGGCGTCATG AACGAGGTGT CTGAGAAGGA CAAGGATGGA GTCATTGAGC
    TCCATAACCA CTGCTCAAGC GTGTACCAGG AAGGCGATGC TCGTTCTGCT
    CTCATTGTCA TGCTCCAAGA ACTTCAGCAT GCGAAGAAGG GCGTCGATAT
    CTTGTCGGGA ACTAGTGTGA AGACCCATTT CGCACGACCT AATTGGCGAA
    GCGTCTTCAA GAAGGTTGCG GTCAGCCATG AGAACCAGCG CGTCGGTGTG
    TTCTACTGTG GTGAGCCTGT GCTGGTTCCC CAACTAAGGC AGTTGTCAGC
    AGATTTCACC CACAAGACAA ACACAAGATT TGATTTCCAC AAGGAGAACT
    TCTAATGGTA CAAATTGAGA AATACCCGTG TATGGTTTTG TATGTAGTTC
    TTTATCATGT GAATTATATG GTTTCTAATA TATATAAAGT TGGACAAAAT
    AAATGAAATG ATGGAAGCTA TTTTGTTTTT T

    Gene models with high sequence identity

    1. AT1G09090.2 [+] show detail - respiratory burst oxidase homolog B
      E-value: 0; Score: 457.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam GCN5-related N-acetyltransferase 5.1e-15
    HMMPfam NADPH oxidase Respiratory burst 1.1e-39
    HMMPfam Ferric reductase-like transmembrane component, N-terminal 8.3e-11
    HMMPfam Ferric reductase, NAD binding 1.3e-42
    HMMPfam FAD-binding 8 3e-30
    HMMPanther RESPIRATORY BURST OXIDASE 2.1e-103
    HMMPanther NADPH OXIDASE 2.1e-103
    HMMPanther RESPIRATORY BURST OXIDASE 3.4e-220
    HMMPanther NADPH OXIDASE 3.4e-220
    Gene3D Acyl-CoA N-acyltransferase 2.2e-07
    Gene3D EF-hand-like domain 1.8e-06
    superfamily Acyl-CoA N-acyltransferase 2.8e-14
    superfamily EF-hand 2.2e-18
    superfamily Riboflavin synthase-like beta-barrel 7.3e-16
    superfamily Ferredoxin reductase-like, C-terminal NADP-li 2.2e-10
    FPrintScan Cytochrome b245, heavy chain 2.6e-17
    FPrintScan Cytochrome b245, heavy chain 2.6e-17
    FPrintScan Cytochrome b245, heavy chain 2.6e-17
    ProfileScan GCN5-related N-acetyltransferase 14.678
    ProfileScan EF-HAND 2 11.696
    ProfileScan EF-HAND 2 6.563
    ProfileScan Ferredoxin reductase-type FAD-binding domain 13.651
    PatternScan EF-Hand 1, calcium-binding site 0

    See the detailed result >

    AT1G09090.2

    Model type
    Protein coding
    Short Description
    respiratory burst oxidase homolog B
    Curator Summary
    NADPH-oxidase AtrbohB plays a role in seed after-ripening. Major producer of superoxide in germinating seeds. AtrbohB pre-mRNA is alternatively spliced in seeds in a hormonally and developmentally regulated manner. ABA caused accumulation of AtrbohB-
    Computational Description
    respiratory burst oxidase homolog B (RBOHB); FUNCTIONS IN: NAD(P)H oxidase activity; INVOLVED IN: response to heat, seed germination, defense response; LOCATED IN: integral to membrane, membrane; EXPRESSED IN: embryo, root; EXPRESSED DURING: imbibition; CONTAINS InterPro DOMAIN/s: Ferredoxin reductase-type FAD-binding domain (InterPro:IPR017927), Cytochrome b245, heavy chain (InterPro:IPR000778), EF-hand-like domain (InterPro:IPR011992), Ferric reductase-like transmembrane component, N-terminal (InterPro:IPR013130), Ferric reductase, NAD binding (InterPro:IPR013121), NADPH oxidase Respiratory burst (InterPro:IPR013623), EF-HAND 2 (InterPro:IPR018249), FAD-binding 8 (InterPro:IPR013112), Riboflavin synthase-like beta-barrel (InterPro:IPR017938); BEST Arabidopsis thaliana protein match is: respiratory burst oxidase homologue D (TAIR:AT5G47910.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR