R90251

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: R90251

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1 whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
rosette leaf (PO:0000014)
  • epinastic (PATO:0000945)
    (Rosette leaf::Shape::epinastic)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: R90251

Determination of introduced cDNA(s)

  • J013084G19 - Rice Full-length cDNAs - Original: J013084G19

    full reading [+] show sequence

    Nucleotide sequence: J013084G19 - full reading 
    GACACACAGC AGGTAGCTAG CTTAGCCCCT CACTTTGTGG CAGATCATAC 
    TCGCTGGCTA CACCACCACC CACATCCATC TGAGCAGTGG AATCATCATC 
    TCCTCCTATA TCAGTTGTCA TCACCCGGCC TGTAGTGTGC GCTCTGATCC 
    TAGTTAGCTG CTGCTCTTCG TTCCGATCCT AGCTCGTTGC GAATTGAGCC 
    AACGGATCAA TCGCGCGCTC CGGCCGCCAC AGCCTTTATT ACCCAAGCTG 
    TTTCTTTTCC AGAATTTAAA GAAGTAGCAG CTGTAGACAG TAGTTATCCT 
    CGTCGCGACC CCTTGTGTGC AGAGCAGGCG GCGGCGGCGG CGTTAGCTAG 
    CTCGTCGAGC AACATATATG GCAGCTAGTA GTAGCACGTA GTTACTGCAG 
    TGAGGAATTC GTCCGGAATC GCGTTGGGAG CGCGCGGAGT AGCGGACGGC 
    TGAGCGGAGC GCTCTCGGTT TGATAAGGGT TCGCGTGTGT GTGTTCGGTG 
    TGGGGGAGAG TAATTGCACG CATCACAAGA ACACTCCTCG CCTCTACTTG 
    GGCTCTCCTG CTCACGTGAT TCATACTCTC CCTACTCCCC TCTCCCCACA 
    TCCGTTGCGC CTTGCATCTA CTCTGTGCCA ATCACCGGGC ATATATACAT 
    GGTTGTGCCT GTGCCGACGA CGTTTGAGGG AGCGAGAGAG ATGGAGGTGG 
    TGGAGGTGCG TGAGTACAGG GAGGACCGGG ACCGCGCCGC CGTCGAGGAG 
    GTGGAGCGGG AGTGCGAGGT GGGCTCCTCC GGCGGCGGCG AAGCCAAGAT 
    GTGCCTGTTC ACGGATCTCC TCGGCGACCC GCTCTGCCGC ATTCGCAACT 
    CGCCGGCCTA CCTCATGCTG GTAGCGGAGA CAGCGAACGG CGGCGGCGGC 
    GGCAATGGCA GGGAGATCAT CGGCCTCATC CGCGGTTGCG TCAAGACCGT 
    CGTCTCCGGC GGCAGCGTCC AGGCCGGCAA GGACCCCATC TACTCCAAGG 
    TCGCCTACAT CCTCGGCCTT CGCGTCTCGC CTCGTTACCG GAGGAAGGGG 
    GTGGGGAAGA AGCTGGTGGG CAGGATGGAG GAGTGGTTCC GGCAGAGCGG 
    GGCGGAGTAC TCGTACATGG CGACGGAGCA GGACAACGAG GCGTCGGTGC 
    GCCTCTTCAC CGGCCGCTGC GGCTACTCCA AGTTCCGGAC GCCGTCGGTG 
    CTCGTGCACC CGGCTCAAGA TGAAGAGCTC CAGCAGGAAG GCGCCATCCC 
    CGCAGGCGCA GCAGTCTGCG AAGAGGGTGA GGAAGAGGCT GGACAGGACC 
    AAGAGCAGCG CCGCCGTGGC GCTCAAAGGA TTGCAGTTTG TGACTGCAAA 
    GGTTGGCAAT GACGGCTGGG CCGCGGTGGA GAAGCGGTTC AATCAGCTGC 
    AGGTGGATGG TGTGCTGCTC CGTTCAAGAT TTGGGAAATG CATTGGAATG 
    GATGGGTCCG ACGAGTTTGC GGTGCAAATG TTCGATTCTC TGGCGAGGAA 
    GAGAGGGATA GTGAAGCAGG TGCTCACTAA GGACGAGCTC AAAGATTTCT 
    ATGAGCAATT GACTGATCAG GGGTTTGACA ATCGTCTTCG GACATTCTTT 
    GACATGGTTG ACAAGAACGC TGATGGAAGG CTCACAGCAG AAGAGGTTAA 
    GGAGATTATT GCCCTTAGTG CATCAGCAAA CAAACTTTCC AAGATCAAGG 
    AGCGAGCTGA TGAGTACACA GCACTCATTA TGGAAGAGCT TGACCCTACA 
    AACTTGGGAT ACATCGAGAT GGAGGACTTG GAAGCACTAT TGCTTCAGTC 
    ACCATCTGAA GCTGCTGCAA GATCAACAAC GACGCACAGC TCCAAACTTA 
    GCAAAGCTCT TAGCATGAAG CTTGCGTCTA ACAAAGAAAT GAGCCCAGTT 
    CGTCATTACT GGCAGCAGTT CATGTACTTC CTTGAAGAGA ATTGGAAGCG 
    CAGTTGGGTT ATGACTCTGT GGATCTCAAT CTGCATTGCC CTTTTCATTT 
    GGAAGTTCAT TCAGTACCGT AATCGAGCCG TATTCGGCAT CATGGGTTAT 
    TGTGTGACCA CTGCAAAGGG TGCTGCAGAG ACCCTCAAAT TCAACATGGC 
    TTTGGTCCTA CTTCCTGTCT GCAGAAATAC AATCACATGG ATTCGGTCAA 
    AGACACAGGT TGGAGCTGTT GTACCCTTCA ACGACAATAT AAACTTTCAT 
    AAGGTCATAG CCGCAGGTGT TGCAGTTGGT GTTGCTTTGC ATGCAGGTGC 
    TCATCTGACA TGTGATTTTC CCCGGCTGCT CCATGCGAGT GATGCACAAT 
    ATGAACTAAT GAAGCCCTTC TTTGGGGAGA AGAGGCCACC AAATTACTGG 
    TGGTTTGTAA AGGGAACTGA AGGCTGGACA GGTGTGGTCA TGGTGGTGCT 
    CATGGCAATA GCATTTACAT TAGCCCAACC ATGGTTCCGA CGTAACAAGC 
    TCAAGGACTC CAATCCCCTC AAAAAAAATG ACTGGCTTCA ATGCCTTCTG 
    GTTTACCCAC CACCTGTTTG TCATTGTGTA CACTTTGCTC TTTGTCCATG 
    GAACGTGCTT GTATCTAAGC AGGAAATGGT ACAAGAAGAC GACATGGATG 
    TACCTCGCTG TTCCTGTTGT CCTGTATGTA AGTGAGCGTA TTCTTCGGTT 
    GTTTAGGAGC CATGATGCAG TTGGGATTCA GAAGGTTGCA GTGTATCCCG 
    GGAATGTATT GGCTCTTTAT ATGTCGAAGC CACCTGGTTT CAGATACCGT 
    AGTGGGCAGT ACATCTTCAT AAAATGCACT GCTGTGTCTC CATATGAATG 
    GCATCCATTT TCCATAACAT CAGCACCTGG AGATGATTAT CTTAGTGTTC 
    ATATTCGCAC AAGGGGTGAT TGGACTTCAC GGCTTAGAAC TGTTTTCTCT 
    GAGGCATGCC GACCCCCCAC TGAGGGAGAA AGTGGACTAC TTAGAGCTGA 
    CCTTTCCAAG GGAATAACGG ACGAAAAAGC AAGATTCCCA AAACTTTTGG 
    TCGATGGACC GTATGGTGCA CCGGCACAAG ATTACCGTGA ATACGATGTG 
    CTACTTCTCA TCGGGCTGGG CATCGGAGCC ACCCCTTTGA TTAGCATTGT 
    GAAGGACGTG CTTAACCACA TTCAAGGTGA GGGATCAGTT GGAACCACGG 
    AGCCGGAGAG CAGCAGCAAG GCGAAGAAGA AACCTTTCAT GACGAAGAGA 
    GCCTACTTCT ACTGGGTGAC GAGAGAGGAG GGCTCGTTTG AGTGGTTCAG 
    AGGCGTCATG AACGAGGTGT CTGAGAAGGA CAAGGATGGA GTCATTGAGC 
    TCCATAACCA CTGCTCAAGC GTGTACCAGG AAGGCGATGC TCGTTCTGCT 
    CTCATTGTCA TGCTCCAAGA ACTTCAGCAT GCGAAGAAGG GCGTCGATAT 
    CTTGTCGGGA ACTAGTGTGA AGACCCATTT CGCACGACCT AATTGGCGAA 
    GCGTCTTCAA GAAGGTTGCG GTCAGCCATG AGAACCAGCG CGTCGGTGTG 
    TTCTACTGTG GTGAGCCTGT GCTGGTTCCC CAACTAAGGC AGTTGTCAGC 
    AGATTTCACC CACAAGACAA ACACAAGATT TGATTTCCAC AAGGAGAACT 
    TCTAATGGTA CAAATTGAGA AATACCCGTG TATGGTTTTG TATGTAGTTC 
    TTTATCATGT GAATTATATG GTTTCTAATA TATATAAAGT TGGACAAAAT 
    AAATGAAATG ATGGAAGCTA TTTTGTTTTT T

    Gene models with high sequence identity

    1. AT1G09090.2 [+] show detail - respiratory burst oxidase homolog B
      E-value: 0; Score: 457.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam GCN5-related N-acetyltransferase 5.1e-15
    HMMPfam NADPH oxidase Respiratory burst 1.1e-39
    HMMPfam Ferric reductase-like transmembrane component, N-terminal 8.3e-11
    HMMPfam Ferric reductase, NAD binding 1.3e-42
    HMMPfam FAD-binding 8 3e-30
    HMMPanther RESPIRATORY BURST OXIDASE 2.1e-103
    HMMPanther NADPH OXIDASE 2.1e-103
    HMMPanther RESPIRATORY BURST OXIDASE 3.4e-220
    HMMPanther NADPH OXIDASE 3.4e-220
    Gene3D Acyl-CoA N-acyltransferase 2.2e-07
    Gene3D EF-hand-like domain 1.8e-06
    superfamily Acyl-CoA N-acyltransferase 2.8e-14
    superfamily EF-hand 2.2e-18
    superfamily Riboflavin synthase-like beta-barrel 7.3e-16
    superfamily Ferredoxin reductase-like, C-terminal NADP-li 2.2e-10
    FPrintScan Cytochrome b245, heavy chain 2.6e-17
    FPrintScan Cytochrome b245, heavy chain 2.6e-17
    FPrintScan Cytochrome b245, heavy chain 2.6e-17
    ProfileScan GCN5-related N-acetyltransferase 14.678
    ProfileScan EF-HAND 2 11.696
    ProfileScan EF-HAND 2 6.563
    ProfileScan Ferredoxin reductase-type FAD-binding domain 13.651
    PatternScan EF-Hand 1, calcium-binding site 0

    See the detailed result >

    AT1G09090.2

    Model type
    Protein coding
    Short Description
    respiratory burst oxidase homolog B
    Curator Summary
    NADPH-oxidase AtrbohB plays a role in seed after-ripening. Major producer of superoxide in germinating seeds. AtrbohB pre-mRNA is alternatively spliced in seeds in a hormonally and developmentally regulated manner. ABA caused accumulation of AtrbohB-
    Computational Description
    respiratory burst oxidase homolog B (RBOHB); FUNCTIONS IN: NAD(P)H oxidase activity; INVOLVED IN: response to heat, seed germination, defense response; LOCATED IN: integral to membrane, membrane; EXPRESSED IN: embryo, root; EXPRESSED DURING: imbibition; CONTAINS InterPro DOMAIN/s: Ferredoxin reductase-type FAD-binding domain (InterPro:IPR017927), Cytochrome b245, heavy chain (InterPro:IPR000778), EF-hand-like domain (InterPro:IPR011992), Ferric reductase-like transmembrane component, N-terminal (InterPro:IPR013130), Ferric reductase, NAD binding (InterPro:IPR013121), NADPH oxidase Respiratory burst (InterPro:IPR013623), EF-HAND 2 (InterPro:IPR018249), FAD-binding 8 (InterPro:IPR013112), Riboflavin synthase-like beta-barrel (InterPro:IPR017938); BEST Arabidopsis thaliana protein match is: respiratory burst oxidase homologue D (TAIR:AT5G47910.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR