R90276

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T1	whole plant (PO:0000003)	decreased height (PATO:0000569) (Adult plant::Plant height::short)
	rosette leaf (PO:0000014)	decreased size (PATO:0000587) (Rosette leaf::Shape::small) decreased length (PATO:0000574) (Rosette leaf::Shape::short) increased width (PATO:0000600) (Rosette leaf::Shape::wide)
	stem (PO:0009047)	abnormal (PATO:0000460) (Stem::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: R90276

Determination of introduced cDNA(s)

J013163D13 - Rice Full-length cDNAs - Original: J013163D13

full reading [+] show sequence

GATCCGTCCT CCTCCTCCTC CTCTCTTCCA TCGCCGCTGC TCGCTTCCTC 
CCCCTCTCCC ACGAAAGATG GCGTCTCACA TTGTTGGGTA CCCCCGCATG 
GGCCCCAAGA GGGAGCTCAA GTTTGCCCTG GAGTCTTTCT GGGATGGGAA 
GAGCAGCGCT GAGGATTTGG AGAAGGTTGC GACCGACCTC AGGGCCAGCA 
TCTGGAAGCA AATGGCTGAC GCTGGGATCA AGTACATCCC CAGCAACACC 
TTCTCGTACT ATGATCAGGT TCTTGACACG GCTGCCATGC TTGGTGCTGT 
CCCAGAGCGC TACTCATGGA CTGGTGGAGA GATTGGGTTC AGCACCTACT 
TCTCGATGGC TAGGGGCAAT GCCACTGTCC CTGCCATGGA GATGACCAAG 
TGGTTTGACA CCAACTACCA CTTTATCGTC CCTGAACTTG GTCCCAACAC 
CAAGTTCTCC TACTCTTCCC ACAAGGCTGT GAATGAATAC AAGGAGGCTA 
AGGCGCTCGG CGTTGACACC GTGCCAGTAC TTGTTGGACC AGTTTCATAC 
TTGTTGCTCT CCAAACCTGC CAAGGGTGTA GAGAAGTCGT TCGCTCTTCT 
TTCCCTTCTG AGCAGCATCC TTCCTGTCTA CAAGGAGGTT ATTGCTGAGT 
TGAAGGCAGC TGGTGCTACT TGGATTCAGT TTGATGAACC CACACTTGTT 
CTTGACCTTG ACTCTCACCA ATTGGCTGCA TTCTCTGCTG CATACACAGA 
ACTTGAGTCG GCACTTTCTG GATTGAATGT GCTTATTGAG ACATACTTCG 
CTGACATTCC TGCTGAATCA TACAAGACCC TCACATCCCT GAACAGCGTG 
ACTGCTTATG GTTTTGACCT TATCCGTGGA GCCAAGACTC TTGACCTTAT 
CAAGAGTGCT GGTTTCCCCT CTGGAAAGTA CCTCTTTGCC GGTGTTGTGG 
ATGGACGCAA CATCTGGGCT GATGATCTCG CTGCATCTCT CACTACCCTT 
GAGTCTCTTG AGGCTATTGT TGGAAAGGAC AAGCTTGTGG TCTCGACTTC 
CTGCTCACTC ATGCACACCG CTGTGGATCT TGTAAATGAG ACCAAGCTCG 
ATAGCGAGAT TAAGTCATGG CTCGCTTTTG CTGCCCAAAA GGTGGTTGAG 
GTGAATGCCC TTGCTAAGGC ATTGGCTGGA CAGAAGGATG AGGCTTACTT 
TGCAGCAAAC GCTGCTGCTC AGGCCTCCAG GAGGTCATCA CCTCGTGTGA 
CAAACGAGGA AGTCCAAAAG GCTGCTGCTG CTCTGAAGGG GTCTGACCAC 
CGCCGTGCTA CCAATGTTAG CGCTAGACTG GACGCACAGC AGAAGAAGCT 
CAACCTTCCA GTCCTTCCTA CAACCACAAT TGGTTCATTC CCACAGACTG 
TGGAGCTCAG GAGAGTCCGC CGTGAGTACA AGGCAAAAAA GATCTCTGAG 
GAGGAGTACA TCAGTGCCAT CAAGGAGGAA ATCAGCAAGG TTGTTAAGAT 
CCAGGAAGAG CTTGACATCG ATGTCCTTGT TCACGGCGAG CCTGAGAGAA 
ACGATATGGT TGAGTACTTC GGTGAGCAGC TTTCTGGTTT TGCATTCACT 
GCCAACGGAT GGGTGCAATC TTATGGATCA CGGTGTGTCA AGCCACCAAT 
TATCTATGGT GATGTGAGCC GCCCCAACCC CATGACTGTG TTCTGGTCCA 
AGTTGGCGCA GAGCATGACC TCTCGCCCCA TGAAGGGAAT GTTGACTGGT 
CCCGTCACAA TCCTTAACTG GTCTTTTGTC AGGAATGACC AGCCGAGGTT 
TGAGACATGC TACCAGATTG CTCTTGCCAT CAAGAAGGAG GTCGAGGACC 
TTGAGGCTGG TGGTATTCAG GTCATCCAAA TCGATGAGGC TGCTTTGAGA 
GAAGGTCTGC CACTCCGCAA GGCGGAGCAT GCTTTCTACT TGGACTGGGC 
TGTTCACTCC TTCAGAATCA CCAACTGCGG TGTTCAGGAC ACCACCCAGA 
TCCACACCCA CATGTGCTAC TCTAACTTCA ACGACATCAT CCACTCCATC 
ATCAACATGG ACGCTGATGT GATCACCATC GAGAACTCCC GGTCCGACGA 
GAAGCTGCTC TCCGTCTTCC GCGAGGGTGT GAAGTATGGT GCTGGCATTG 
GCCCTGGTGT CTATGACATC CACTCCCCAA GGATCCCGTC CACAGAGGAG 
ATTGCGGACC GCGTCAACAA GATGCTCGCG GTGCTCGACA CTAACATTCT 
CTGGGTGAAC CCTGACTGCG GTCTCAAGAC CCGCAAGTAC AACGAGGTCA 
AGCCCGCCCT TACCAACATG GTTTCGGCCG CCAAGCTCAT CCGCACCCAG 
CTCGCCAGCG CGAAGTGAGG ATATCCATAG CTGCAAGTTC CCTTCGGTTG 
CGAGGACGAT GCCGTCATAG CCATCTGTTT TTGAATAATC TGGGTCCAGT 
GGATCCAATT GTCTGTGGTT AGGTTTAGTC AGTCTCGGTG GGAAGATCCC 
TCATACCCGT TTTACTTTTG AATCAATTTG GTGGTTTTTG AGGCAGTTTG 
TCGGTGTATT TGTATTGTGT GTAATCTGAG TGGGAATCAG CAGTGTTTCA 
TCTGCATCTG AATCGTTGCG GATCAAATTA CAAAATTTTC

Gene models with high sequence identity

AT5G17920.2 [+] show detail - Cobalamin-independent synthase family protein
E-value: 0; Score: 999.99

InterPro Scan Digest

Program	Description	E-value
HMMTigr	5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase	0
HMMPfam	Methionine synthase, vitamin-B12 independent	1.3e-148
HMMPfam	Cobalamin (vitamin B12)-independent methionine synthase MetE, N-terminal	1.9e-117
superfamily	UROD/MetE-like	3.4e-129
superfamily	UROD/MetE-like	2.6e-125
HMMPIR	5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase	0
HAMAP	5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase	36.046
Gene3D	no description	4.7e-66
Gene3D	no description	3.4e-104

See the detailed result >

AT5G17920.2

Model type: Protein coding
Short Description: Cobalamin-independent synthase family protein
Curator Summary: Encodes a cytosolic cobalamin-independent methionine synthase, involved in methionine regeneration via the activated methyl cycle (SAM cycle). The protein undergoes thiolation following treatment with the oxidant tert-butylhydroperoxide.
Computational Description: COBALAMIN-INDEPENDENT METHIONINE SYNTHASE (ATCIMS); FUNCTIONS IN: 5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity, methionine synthase activity; INVOLVED IN: response to zinc ion, response to salt stress, methionine biosynthetic process; LOCATED IN: in 6 components; EXPRESSED IN: 9 plant structures; EXPRESSED DURING: seedling growth, seed development stages; CONTAINS InterPro DOMAIN/s: Cobalamin (vitamin B12)-independent methionine synthase MetE, N-terminal (InterPro:IPR013215), Methionine synthase, vitamin-B12 independent (InterPro:IPR002629), 5-methyltetrahydropteroyltriglutamate--homocysteine S-methyltransferase (InterPro:IPR006276); BEST Arabidopsis thaliana protein match is: methionine synthase 2 (TAIR:AT3G03780.3); Has 4442 Blast hits to 4434 proteins in 1682 species: Archae - 183; Bacteria - 3246; Metazoa - 14; Fungi - 206; Plants - 251; Viruses - 2; Other Eukaryotes - 540 (source: NCBI BLink).
Link: InterPro Scan - TAIR