R90307

RIKEN rice full-length cDNA overexpressed Arabidopsis lines

Original: R90307

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1 cauline leaf (PO:0000013)
  • desaturated green (PATO:0001272)
    (Cauline leaf::Color::pale)
rosette leaf (PO:0000014)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
  • desaturated green (PATO:0001272)
    (Rosette leaf::Color::pale)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: R90307

Determination of introduced cDNA(s)

  • J013001J18 - Rice Full-length cDNAs - Original: J013001J18

    full reading [+] show sequence

    Nucleotide sequence: J013001J18 - full reading
    GGCTTGCACA CTTGCACCCA CCCTCTCTCC TCCATCCAGC ACCGACCATG 
    GCCGCCGTCA CGGCTGCGGC CGTCTCCACC TCCGCCGCTG CTGCAGTCAC
    CAAGGCATCG CCGTCCCCCG CCCACTGCTT CCTGCCATGC CCGCCAAGAA
    CCAGAGCCGC CCACCAGCGC GGCCTGCTGC TGCGCGCGCA GGTGTCCACC
    ACCGACGCCG CCGCCGTCGC CGCCGCGCCG GCCAAGAAGG AGAAGATATC
    CAAGAAGCAT GACGAGGGCG TCGTCACCAA CAAGTACAGG CCCAAGGAGC
    CCTACGTCGG CAAGTGCCTC CTCAACACCA AGATCACCGC CGACGACGCG
    CCCGGCGAGA CATGGCACAT GGTCTTCAGC ACCGAGGGTG AGATCCCCTA
    CAGAGAGGGG CAGTCCATCG GCGTCATCGC CGACGGCGTC GACAAGAACG
    GCAAGCCGCA CAAGCTCAGG CTCTACTCCA TCGCCAGCAG CGCTCTCGGC
    GACTTCGGCG ACTCCAAGAC CGTTTCACTC TGCGTCAAGA GGCTCGTTTA
    CACCAACGAC CAGGGAGAGA TTGTCAAAGG AGTCTGCTCC AACTTCCTCT
    GTGACTTGAA GCCTGGTTCT GATGTCAAGA TAACCGGACC AGTAGGCAAA
    GAAATGCTCA TGCCCAAAGA TCCCAATGCT AATATTATAA TGCTTGCGAC
    CGGTACTGGT ATTGCCCCGT TCCGCTCATT CTTGTGGAAA ATGTTTTTTG
    AGAAGTATGA TGACTACAAG TTCAATGGTC TGGCTTGGCT CTTCTTGGGA
    GTCCCAACTA GCAGTTCTTT ACTCTACAAG GAGGAGTTTG ACAAAATGAA
    GGCGAAAGCG CCAGAGAACT TCCGGGTCGA TTATGCTGTG AGCAGGGAGC
    AGACCAATGC TCAAGGAGAG AAGATGTACA TTCAGACCAG GATGGCAGAG
    TACAAGGAAG AGCTGTGGGA GCTCCTGAAG AAGGACCACA CCTATGTGTA
    CATGTGTGGA CTGAAAGGCA TGGAGAAGGG TATTGATGAC ATTATGGTGT
    CATTGGCTGC AAAAGATGGA ATCGACTGGG CTGATTACAA GAAGCAACTG
    AAGAAGGGCG AGCAATGGAA CGTGGAAGTC TACTAATTCT TCCAATTTTC
    CTCACATCTG TTTCTTTTTT TTCTTCCATT TGTATCTGTG TGCACATCTG
    TGCCTGTGAT CACTCTATAA TGTTAGATAG GCGTATATAT ATACTGTTTG
    TCATGTTGGT TAAATTCAAG CTTCATATAA GAATTACTAC TTATGTCTGA
    TCCAAATACT ACTATGGTCA AGTCAAGAGT AATAATAATA ATAATGCAAT
    GCAGTTCACT C

    Gene models with high sequence identity

    1. AT1G20020.1 [+] show detail - ferredoxin-NADP(+)-oxidoreductase 2
      E-value: 2e-154; Score: 542.00

     

    InterPro Scan Digest

    Program Description E-value
    Coil coiled-coil 0
    HMMPfam Oxidoreductase FAD/NAD(P)-binding 4e-27
    HMMPfam Oxidoreductase, FAD-binding domain 5.8e-05
    FPrintScan Flavoprotein pyridine nucleotide cytochrome reductase 1.2e-26
    FPrintScan Flavoprotein pyridine nucleotide cytochrome reductase 1.2e-26
    FPrintScan Flavoprotein pyridine nucleotide cytochrome reductase 1.2e-26
    FPrintScan Flavoprotein pyridine nucleotide cytochrome reductase 1.2e-26
    FPrintScan Flavoprotein pyridine nucleotide cytochrome reductase 1.2e-26
    FPrintScan Flavoprotein pyridine nucleotide cytochrome reductase 1.2e-26
    FPrintScan Flavoprotein pyridine nucleotide cytochrome reductase 1.2e-26
    FPrintScan Flavoprotein pyridine nucleotide cytochrome reductase 1.2e-26
    HMMPIR Ferredoxin--NADP reductase 2.8e-274
    ProfileScan Ferredoxin reductase-type FAD-binding domain 13.279
    Gene3D no description 1.4e-48
    Gene3D no description 2.2e-47
    superfamily Ferredoxin reductase-like, C-terminal NADP-li 9.3e-54
    superfamily Riboflavin synthase-like beta-barrel 3e-43
    HMMPanther Ferredoxin Reductase 1e-138
    HMMPanther FLAVODOXIN-RELATED 1e-138

    See the detailed result >

    AT1G20020.1

    Model type
    Protein coding
    Short Description
    ferredoxin-NADP(+)-oxidoreductase 2
    Curator Summary
    Encodes a leaf-type ferredoxin:NADP(H) oxidoreductase. It is present in both chloroplast stroma and thylakoid membranes but is more abundant in the stroma
    Computational Description
    ferredoxin-NADP(+)-oxidoreductase 2 (FNR2); FUNCTIONS IN: oxidoreductase activity, poly(U) RNA binding, NADPH dehydrogenase activity; INVOLVED IN: oxidation reduction, defense response to fungus, incompatible interaction, defense response to bacterium; LOCATED IN: thylakoid, chloroplast thylakoid membrane, apoplast, chloroplast stroma, chloroplast; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Oxidoreductase FAD/NAD(P)-binding (InterPro:IPR001433), Ferredoxin reductase-type FAD-binding domain (InterPro:IPR017927), Oxidoreductase, FAD-binding domain (InterPro:IPR008333), Riboflavin synthase-like beta-barrel (InterPro:IPR017938), Ferredoxin Reductase (InterPro:IPR015701), Flavoprotein pyridine nucleotide cytochrome reductase (InterPro:IPR001709), Ferredoxin--NADP reductase (InterPro:IPR012146); BEST Arabidopsis thaliana protein match is: ferredoxin-NADP(+)-oxidoreductase 1 (TAIR:AT5G66190.1); Has 6774 Blast hits to 6774 proteins in 1708 species: Archae - 20; Bacteria - 3604; Metazoa - 802; Fungi - 759; Plants - 616; Viruses - 0; Other Eukaryotes - 973 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR