F00627

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F00627

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
rosette leaf (PO:0000014)
  • desaturated green (PATO:0001272)
    (Rosette leaf::Color::pale)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
sporophyte vegetative stage (PO:0007134)
  • decreased speed (PATO:0000304)
    (Adult plant::Growth::slow)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F00627

Determination of introduced cDNA(s)

  • RAFL06-13-O22 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL06-13-O22 - Resource: pda09410

    full reading [+] show sequence

    Nucleotide sequence: RAFL06-13-O22 - full reading 
    ACTCAACCAA TTTCTTCTCA GGTAATACTC GTAAGATTTA TCAACACAAG 
    CAAAAAAGAT GGCCCAAAAG CTAGAAGCAA AGGGTGGTGA GATGGGAGAT 
    GTATGGGATG ATGGTGTTTA CGAAAATGTT AGAAAGGTAT ATGTAGGGCA 
    AGCACAATAC GGTATAGCCT TCGTCAAGTT TGAGTATGTC AATGGTTCTC 
    AAGTGGTTGT TGGAGATGAA CATGGAAAGA AGACAGAGCT AGGAGTTGAA 
    GAGTTTGAGA TTGATGCGGA TGACTACATC GTATACGTGG AAGGTTACCG 
    TGAGAAAGTT AACGATATGA CGTCAGAAAT GATCACGTTT CTTTCTATTA 
    AGACTTTTAA AGGCAAAACC TCTCATCCTA TCGAAAAAAG ACCTGGGGTT 
    AAGTTTGTGC TACATGGTGG AAAAATCGTT GGGTTTCACG GACGTTCAAC 
    AGATGTTTTA CACTCCCTTG GGGCCTATGT TTCTTTGTCA TCCACTATCA 
    AATTGCTTGG GAAGTGGATT AAGGTGGAGC AAAAAGGAGA AGGTCCAGGG 
    CTAAGATGCT CACATGGCAT AGCACAAGTA GGCAACAAGA TTTACTCCTT 
    TGGTGGCGAG TTCACACCAA ATCAGCCCAT CGACAAACAC CTTTACGTCT 
    TTGACCTCGA GACCCGGACT TGGTCCATTT CTCCAGCCAC CGGAGACGTT 
    CCACACCTCT CTTGTTTAGG TGTCCGGATG GTGTCAGTAG GATCGACCCT 
    CTATGTCTTT GGAGGCCGAG ACGCTTCACG CCAATACAAC GGTTTCTACT 
    CGTTTGACAC GACCACTAAC GAGTGGAAAC TGCTAACTCC GGTCGAAGAA 
    GGACCCACTC CTCGTAGTTT CCACTCAATG GCAGCCGATG AGGAAAACGT 
    TTACGTTTTC GGTGGAGTGA GTGCTACGGC ACGACTCAAT ACACTAGACT 
    CTTACAACAT CGTTGATAAG AAGTGGTTTC ATTGTTCGAC TCCAGGAGAT 
    TCCCTTACCG CAAGAGGAGG AGCAGGGCTC GAAGTGGTGC AAGGGAAGGT 
    ATGGGTTGTG TATGGGTTTA ACGGATGTGA AGTAGATGAT GTTCATTACT 
    ACGATCCTGT TCAAGACAAG TGGACACAAG TGGAAACATT CGGTGTGAGG 
    CCTTCCGAAA GAAGTGTATT CGCTAGTGCG GCTATTGGGA AACACATTGT 
    GATTTTTGGA GGTGAAATTG CGATGGACCC GCTAGCTCAC GTGGGTCCAG 
    GTCAATTGAC CGATGGAACT TTTGCATTGG ACACAGAGAC GTTGCAATGG 
    GAGAGGCTGG ATAAGTTTGG TGGAGAGGAA GAGACTCCGA GTAGCAGAGG 
    ATGGACCGCG TCCACGACTG CTACCATTGA TGGTAAGAAA GGACTTGTGA 
    TGCATGGTGG TAAAGCTCCA ACCAATGACC GGTTTGATGA TCTCTTCTTT 
    TACGGGATTG ACTCTGCCTA ATTAAGGCAG ATTTGGTGAT CATGAACGTT 
    TGTGAATGTG TGTGTGTGTG TGTGGGTCTG TGTTTGTGTG TGTAATAAGG 
    GAGGGGTATA CCACCATATG ATCCTTCCCC AGCTTGACCA TTGTTTTTTT 
    TTTAATATGT TTTTCTTTCT ACATGTTTGT TTCACGTTTC ATGTTGTATC 
    CAATAAATTG TGTACTTTCC TAGTAGTAAT CGCCAGAAAA AAAAAAAGG

    Gene models with high sequence identity

    1. AT3G16400.1 [+] show detail - nitrile specifier protein 1
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMSmart Kelch repeat type 1 0.41
    HMMSmart Kelch repeat type 1 0.26
    HMMSmart Kelch repeat type 1 11
    superfamily Galactose oxidase/kelch, beta-propeller 8.5e-39
    superfamily Mannose-binding lectin 3.8e-38
    HMMPfam Mannose-binding lectin 9.3e-41
    HMMPfam Kelch repeat type 1 3.3e-07
    HMMPfam Kelch repeat type 1 1.7e-06
    HMMPfam Kelch repeat type 1 1.1e-07
    HMMPfam Kelch repeat type 1 1.2e-07
    Gene3D Mannose-binding lectin 1.7e-33
    Gene3D Kelch-type beta propeller 1.1e-45
    HMMPanther JASMONATE INDUCIBLE PROTEIN-RELATED 0
    HMMPanther KELCH REPEAT DOMAIN 0

    See the detailed result >

    AT3G16400.1

    Model type
    Protein coding
    Short Description
    nitrile specifier protein 1
    Curator Summary
    Encodes a nitrile-specifier protein NSP1 responsible for constitutive and herbivore-induced simple nitrile formation in rosette leaves. NSP1 is one out of five (At3g16400/NSP1, At2g33070/NSP2, At3g16390/NSP3, At3g16410/NSP4 and At5g48180/NSP5) A. thaliana epithiospecifier protein (ESP) homologues that promote simple nitrile, but not epithionitrile or thiocyanate formation.
    Computational Description
    nitrile specifier protein 1 (NSP1); FUNCTIONS IN: molecular_function unknown; INVOLVED IN: glucosinolate catabolic process, response to herbivore, nitrile biosynthetic process; LOCATED IN: cellular_component unknown; EXPRESSED IN: rosette leaf, cotyledon, guard cell, leaf; EXPRESSED DURING: seedling growth; CONTAINS InterPro DOMAIN/s: Kelch repeat type 1 (InterPro:IPR006652), Galactose oxidase/kelch, beta-propeller (InterPro:IPR011043), Myrosinase-binding related, jacalin-like lectin (InterPro:IPR017388), Mannose-binding lectin (InterPro:IPR001229), Kelch-type beta propeller (InterPro:IPR015915); BEST Arabidopsis thaliana protein match is: nitrile specifier protein 4 (TAIR:AT3G16410.1); Has 12798 Blast hits to 6850 proteins in 422 species: Archae - 13; Bacteria - 486; Metazoa - 7547; Fungi - 708; Plants - 2515; Viruses - 41; Other Eukaryotes - 1488 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR