F00736

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F00736

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
cauline leaf (PO:0000013)
  • abnormal (PATO:0000460)
    (Cauline leaf::Others)
rosette leaf (PO:0000014)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
sporophyte vegetative stage (PO:0007134)
  • decreased speed (PATO:0000304)
    (Adult plant::Growth::slow)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F00736

Determination of introduced cDNA(s)

  • RAFL06-11-G09 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL06-11-G09 - Resource: pda03074

    full reading [+] show sequence

    Nucleotide sequence: RAFL06-11-G09 - full reading 
    GAGAGCTAAA TCATTTACGT TTTGATCGAC AAAAATGGAG TTGATAAACG 
    ACTTTCTCAA CCTAACTGCT CCTTTCTTCA CCTTCTTTGG TCTCTGCTTC 
    TTCTTGCCTC CTTTTTACTT CTTCAAGTTC TTGCAGTCTA TTTTCTCGAC 
    AATTTTCTCT GAAAATCTTT ACGGGAAAGT TGTTCTCATC ACTGGTGCTT 
    CCTCCGGTAT AGGCGAGCAA TTGGCATATG AGTACGCATG TAGAGGTGCA 
    TGTTTAGCCC TGACCGCCCG AAGGAAGAAC CGTCTAGAGG AAGTGGCAGA 
    GATTGCTCGT GAACTCGGAT CTCCCAATGT TGTTACCGTT CATGCTGATG 
    TCTCCAAACC TGATGACTGT AGACGAATCG TTGATGACAC CATCACCCAT 
    TTTGGCAGAT TGGATCATCT TGTAAATAAT GCTGGGATGA CGCAGATTTC 
    AATGTTTGAG AACATTGAAG ATATAACGAG GACAAAAGCA GTTTTGGATA 
    CTAACTTCTG GGGATCGGTT TATACCACTC GTGCTGCGCT TCCATACCTT 
    AGACAAAGTA ATGGTAAGAT TGTGGCTATG TCGTCTTCCG CAGCGTGGCT 
    AACCGCTCCA AGGATGAGTT TTTACAATGC AAGCAAAGCA GCTTTGTTGA 
    GCTTCTTTGA GACGATGAGA ATTGAGCTTG GTGGCGATGT ACACATTACA 
    ATCGTCACAC CTGGTTATAT CGAGTCGGAG CTCACACAAG GCAAGTACTT 
    CTCTGGTGAA GGCGAGTTAA TAGTCAACCA AGACATGAGA GATGTTCAAG 
    TAGGACCATT TCCGGTAGCG TCAGCATCAG GATGTGCCAA GTCGATAGTG 
    AACGGTGTGT GCCGGAAACA AAGATACGTG ACAGAGCCAT CATGGTTTAA 
    GGTGACGTAC CTTTGGAAAG TGCTATGTCC GGAGTTGATT GAGTGGGGTT 
    GTCGGTTACT GTACATGACT GGAACTGGTA TGTCCGAGGA TACTGCACTC 
    AACAAGAGGA TCATGGACAT TCCTGGTGTA CGTAGTACTC TGTACCCGGA 
    ATCTATCAGA ACTCCAGAAA TCAAGTCGGA TTAGAGAGGG GTCAATACAT 
    AATAAATGGA TGAGATGTAT CATGAGTATG TGCAATGAGT AATAATTATC 
    AAAGAAATGG GTGAAAATGT AGGTTTGTTT TTGTTAATGG TACTTTTGAC 
    TATGTATAT

    Gene models with high sequence identity

    1. AT5G50700.1 [+] show detail - hydroxysteroid dehydrogenase 1
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPanther 11BETA-HYDROXYSTEROID DEHYDROGENASE 3.2e-146
    HMMPanther Short-chain dehydrogenase/reductase SDR 3.2e-146
    Gene3D NAD(P)-binding domain 2.9e-60
    superfamily NAD(P)-binding domain 3.1e-61
    Coil coiled-coil 0
    HMMPfam Short-chain dehydrogenase/reductase SDR 5.5e-32
    FPrintScan Glucose/ribitol dehydrogenase 5.9e-24
    FPrintScan Glucose/ribitol dehydrogenase 5.9e-24
    FPrintScan Glucose/ribitol dehydrogenase 5.9e-24
    FPrintScan Glucose/ribitol dehydrogenase 5.9e-24
    FPrintScan Glucose/ribitol dehydrogenase 5.9e-24
    FPrintScan Short-chain dehydrogenase/reductase SDR 3.9e-08
    FPrintScan Short-chain dehydrogenase/reductase SDR 3.9e-08
    FPrintScan Short-chain dehydrogenase/reductase SDR 3.9e-08
    PatternScan Short-chain dehydrogenase/reductase, conserved site 0

    See the detailed result >

    AT5G50700.1

    Model type
    Protein coding
    Short Description
    hydroxysteroid dehydrogenase 1
    Curator Summary
    Encodes a hydroxysteroid dehydrogenase HSD1. Two copies of HSD1 (At5g50600 and At5g50700) exist in the Arabidopsis genome as a result of an exact 33-kb duplication on chromosome 5 encompassing seven genes. There are five homologs of HSD1 in Arabidopsis (HSD2-At3g47350, HSD3-At3g47360, HSD4-At5g50590, HSD4-At5g50690 and HSD6-At5g50770; HSD4 has two copies due to the same gene duplication event occurred to HSD1) (Plant Cell Physiology 50:1463). At5g50690 is also named as HSD7 (Plant Physiology 145:87). HSD1 is identified from the proteome of oil bodies from mature seeds. Transcription of HSD1 is specifically and highly induced in oil-accumulating tissues of mature seeds; transcript disappears during germination.
    Computational Description
    hydroxysteroid dehydrogenase 1 (HSD1); FUNCTIONS IN: oxidoreductase activity, binding, catalytic activity; INVOLVED IN: oxidation reduction, metabolic process; LOCATED IN: endomembrane system; EXPRESSED IN: seed; CONTAINS InterPro DOMAIN/s: Short-chain dehydrogenase/reductase, conserved site (InterPro:IPR020904), NAD(P)-binding domain (InterPro:IPR016040), Glucose/ribitol dehydrogenase (InterPro:IPR002347), Short-chain dehydrogenase/reductase SDR (InterPro:IPR002198); BEST Arabidopsis thaliana protein match is: hydroxysteroid dehydrogenase 1 (TAIR:AT5G50600.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR