F03131

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T2	seed (PO:0009010)	increased length (PATO:0000573) (Seed::Shape::long)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F03131

Determination of introduced cDNA(s)

RAFL08-08-E02 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL08-08-E02 - Resource: pda04483

full reading [+] show sequence

AAAAAAAATC ATCAAATCTT CTCCTTCTCT CTCTCTCTCT CACACACTCA 
CTAGCTTCTC TGTTAAAAGC TTCCGTCATC ACATAGAGAA CAGAGACTGA 
CACTAACTCA CTTCCTAACG GTTTCCACAC TCAAAATTAC ACTTTTGCCA 
CTCCGGCGAA CAACAATATG ATCACATGGC ACGACCTCTA CACCGTCCTC 
ACGGCGGTGA TACCACTCTA CGTAGCCATG ATCCTCGCTT ACGGCTCAGT 
CCGGTGGTGG AAAATCTTCT CACCAGACCA ATGTTCCGGC ATAAACCGCT 
TCGTCGCTAT TTTCGCCGTC CCTCTTCTCT CTTTCCACTT CATCTCCTCA 
AACAATCCTT ACGCAATGAA TCTCCGATTC ATCGCAGCCG ATACACTCCA 
AAAACTAATC ATGCTCACTC TCCTAATCAT CTGGGCTAAC TTCACTCGCT 
CCGGTAGTCT TGAATGGAGC ATCACAATCT TCTCTCTTTC AACACTTCCC 
AACACTCTCG TTATGGGAAT ACCTCTCTTG ATCGCCATGT ACGGAGAATA 
TTCCGGCTCA CTCATGGTTC AGATCGTTGT TCTTCAGTGT ATAATCTGGT 
ACACGCTTCT TCTCTTTCTT TTCGAATACA GAGGAGCTAA GATCTTGATC 
ATGGAACAGT TTCCAGAGAC TGGTGCTTCG ATTGTATCGT TCAAAGTTGA 
GTCCGATGTT GTTTCTTTAG ATGGACATGA TTTTCTTGAA ACTGATGCTC 
AAATCGGTGA CGATGGTAAG CTTCATGTTA CGGTGAGAAA ATCAAACGCT 
TCTCGGAGAT CTTTTTACGG TGGTGGTGGT ACTAATATGA CTCCTCGTCC 
GTCTAATCTC ACCGGAGCTG AGATTTATAG TCTTAACACT ACTCCAAGAG 
GATCTAACTT CAATCATTCT GATTTTTACT CTATGATGGG GTTTCCCGGT 
GGCCGGCTTT CGAACTTTGG TCCGGCGGAT ATGTACTCTG TTCAGTCCTC 
GAGAGGACCT ACTCCTAGAC CTTCGAATTT TGAGGAGAGT TGTGCTATGG 
CTTCTTCGCC AAGATTCGGG TATTACCCGG GAGGAGCTCC CGGGTCGTAC 
CCAGCTCCTA ATCCGGAGTT TTCTACCGGT AATAAAACCG GTAGTAAAGC 
TCCAAAAGAG AATCATCATC ATGTAGGAAA ATCAAATAGT AATGATGCTA 
AGGAGCTTCA TATGTTTGTT TGGGGATCCA ACGGATCACC CGTTTCGGAC 
CGAGCTGGTC TTCAAGTTGA TAATGGAGCC AATGAACAAG TCGGAAAATC 
CGATCAAGGC GGTGCAAAAG AGATTCGAAT GTTGATCTCT GATCATACTC 
AAAATGCTGG TCCGATGAAC GGGGACTATG GCGGGGAAGA AGAGTCGGAG 
AGGGTAAAGG AAGTGCCTAA CGGACTACAC AAGCTTCGGT GTAACTCCAC 
AGCAGAGCTA AACCCTAAAG AAGCTATAGA AACGGGTGAA ACTGTACCGG 
TAAAACATAT GCCACCAGCG AGTGTGATGA CTCGGCTGAT ATTGATAATG 
GTGTGGAGGA AACTCATAAG AAACCCAAAC ACTTACTCTA GTCTCATTGG 
TCTCATTTGG GCTCTTGTTG CTTTCAGGTG GGATGTGGCA ATGCCTAAAA 
TTATTCAACA ATCAATCTCA ATTCTTTCTG ATGCTGGTCT TGGTATGGCA 
ATGTTCAGTT TGGGTGAGTC ATCTTTTTAC TCTGTTTCTT TTTTCCGTTA 
ACTCATTTAC TTTCTTTCTA TTTTTACGCC AATCAAATGT CACAACATCC 
ACATTCGTAC GGAAAAAATT GTAACTTTTT TTTGGTGAGG ATTCCAATAA 
TTGAAAATGG TTGGTATTTT TTAAGATTTT TTGGGATGGC CCGTTACGGC 
ATGGACCATC CGACAGGTAG GGTCATTGAT GCGACGTCGA ATTATATAAA 
CGGTCGTGGT GGGATGACAT AGCTCGTGTT CTTGATTGTT GTCGTGGTCC 
CCATATTAAG TGGAAGTTGA TTGGACTTAC AATGTACATG AATGCATCAT 
CATCATCATT TATTGGATTT AGATTACGTT TACATGTATT GCATCATTCA 
GCACTTGTTG TTTTGAGTGG AAATGAATTA ACTGAAAATG TGAAAATGGT 
GAAGGGTTGT TCATGGCATT GCAACCGAAA TTAATTGCTT GCGGAAATTC 
GACGGCGACT TTTGCGATGG CGGTGAGATT CTTTACTGGA CCAGCGGTAA 
TGGCCGTGGC AGCAATGGCT ATTGGATTAC GTGGAGACCT ATTGCGTGTG 
GCCATTGTTC AAGTAAATTA AACTATTTTC GTTTGTTGTT AACAATCTCT 
TTGTAAGAAG TAGTTTAACC AAATCTTGTG GTAACGTTGT AGGCTGCATT 
GCCTCAAGGG ATCGTGCCGT TTGTGTTTGC AAAAGAGTAT AATGTTCATC 
CCGCAATCTT GAGTACAGGG TAAGTATTTC ATATTGAACC AATAGTAAAA 
CTTTTCTTAT GCATAAGAGA AGATTACTAA TATTGTTGAT TCTTATGTGT 
ATTATTGCAG GGTAATATTT GGAATGCTTA TCGCACTACC GATTACACTT 
GTTTACTACA TTTTACTCGG GCTATAAGCT CTTCAATGGA GAAGTTCTGC 
TTGGCTGAAG AGGATAAAGA GAAACAAGAT AATTCCAAGA TTAGTGGAAC 
GCAACTAGAA AATGTAATGT AACACGAAAT GTAGTAAATT TAAAGGGACC 
AAGATTTGAT TTGAGGATCC AAAAACCCTT TTCTTGTCTA AG

Gene models with high sequence identity

AT1G23080.2 [+] show detail - Auxin efflux carrier family protein
E-value: 0; Score: 100.00

InterPro Scan Digest

Program	Description	E-value
HMMPfam	Auxin efflux carrier	5.8e-140
HMMPfam	Auxin efflux carrier	4.7e-19
HMMTigr	Auxin efflux carrier, subgroup	9.8e-303

See the detailed result >

AT1G23080.2

Model type: Protein coding
Short Description: Auxin efflux carrier family protein
Curator Summary: Encodes a novel component of auxin efflux that is located apically in the basal cell and is involved during embryogenesis in setting up the apical-basal axis in the embryo. It is also involved in pattern specification during root development. In roots, it is expressed at lateral and basal membranes of provascular cells in the meristem and elongation zone, whereas in the columella cells it coincides with the PIN3 domain. Plasma membrane-localized PIN proteins mediate a saturable efflux of auxin. PINs mediate auxin efflux from mammalian and yeast cells without needing additional plant-specific factors. The action of PINs in auxin efflux is distinct from PGPs, rate-limiting, specific to auxins and sensitive to auxin transport inhibitors. PINs are directly involved of in catalyzing cellular auxin efflux.
Computational Description: PIN-FORMED 7 (PIN7); FUNCTIONS IN: auxin:hydrogen symporter activity, transporter activity, auxin efflux transmembrane transporter activity; INVOLVED IN: response to cyclopentenone, root development, pattern specification process, auxin polar transport, longitudinal axis specification; LOCATED IN: plasma membrane, membrane; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Auxin efflux carrier, subgroup (InterPro:IPR014024), Auxin efflux carrier (InterPro:IPR004776); BEST Arabidopsis thaliana protein match is: Auxin efflux carrier family protein (TAIR:AT1G70940.1); Has 700 Blast hits to 605 proteins in 126 species: Archae - 0; Bacteria - 174; Metazoa - 8; Fungi - 2; Plants - 457; Viruses - 0; Other Eukaryotes - 59 (source: NCBI BLink).
Link: InterPro Scan - TAIR