F05308

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F05308

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
stem (PO:0009047)
  • increased thickness (PATO:0000591)
    (Stem::Shape::thick)
  • dark green (PATO:0001249)
    (Stem::Color::dark)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F05308

Determination of introduced cDNA(s)

  • RAFL06-08-A17 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL06-08-A17 - Resource: pda12245

    full reading [+] show sequence

    Nucleotide sequence: RAFL06-08-A17 - full reading 
    ACACACACTT CTCACTTAAG AAAATGAATA ACTCTTTACA AAGTTCTAAA 
    CTTGTGCTTC TATTGGCTAT TGCTTTGGTC CTATTTCTTA ACACGGAGCT 
    GGATTTTCTT ACAGCTGCTG GAGCCTTAGA CAGTGATAGC AAAGTTTATA 
    TAGTGTATCT TGGCGAAAGA GAACACGATG ATCCTGAACT TGTCACAGCT 
    TCTCATCACC AGATGCTTGA ATCACTTCTT CAAAGCAAAG AAGACGCACA 
    AAACTCCCTG ATCTACAGCT ACCAACATGG ATTCTCTGGT TTCGCGGCGC 
    TTCTTACATC ATCACAAGCT AAGAAAATTT CAGAGCATCC AGAAGTTATC 
    CATGTTATAC CAAACCGGAT TCGGAAACTT AAAACCACAA GAGCTTGGGA 
    TCACCTTGGC CTCTCTCCAA TTCCAACTTC TTTTTCTTCA TTATCATCTG 
    TAAAAGGTCT TCTTCATGAC ACCAACTTGG GCAGTGAAGC TATCATCGGT 
    GTCATTGATT CCGGGATATG GCCAGAGTCA AAGGCAGTCA ACGATCAAGG 
    CCTTGGACCA ATACCTAAGC GTTGGAGAGG AAAATGTGAA CCTGGAGAAC 
    AGTTTAACGC CACAATACAT TGCAACAACA AGCTAATAGG AGCTAGGTAC 
    TATCTAAACG GTGTAGTCGC CGCAATTGGA GGAAAATTCA ACAGAACAAT 
    CATCCAAGAT TTCCAATCCA CCAGAGATGC AAACGGTCAC GGTACACATA 
    CAGCCACAAT AGCTGGTGGC TCATTTGTCC CCAACGTGAG CTACTTTGGT 
    TTAGCCCAAG GTCTTGTCCG AGGTGGTGCT CCTCGAGCCC GAATAGCCTC 
    GTACAAGGCG TGTTGGAACG TAATGAGAGA TGAAGGAGGA GGAACTGATG 
    GAAGATGCAC ATCTGCTGAT ATGTGGAAGG CTTTTGATGA TGCTATACAT 
    GATGGGGTTG ATGTTTTGTC GGTTTCTATC GGGGGAGGTA TTCCCGAGGA 
    TTCGGAAGTC GATAAGCTCG ATTATATCGC GGCTTTTCAC GCGGTGGCTA 
    AAGGGATTAC GGTTGTGGCT GCTGCGGGTA ACGAAGGGCC TGGTGCTCAC 
    ACTGTTGACA ATGTTGCTCC TTGGCTTTTG ACTGTTGCTG CAACTACTCT 
    TGACCGGTCT TTTCCTACAA AGATCACACT TGGGAATAAT CAAACCCTCT 
    TCGCTGAATC TCTGTTCACT GGACCGGAGA TTTCAACCGG TTTAGCTTTC 
    TTGGACTCAG ACAGTGATGA CACTGTTGAT GTGAAGGGGA AAACAGTTCT 
    TGTTTTCGAT AGTGCAACTC CAATTGCAGG GAAAGGTGTA GCAGCAGTGA 
    TCCTAGCCCA AAAGCCTGAT GATCTTCTTT CTCGATGCAA TGGTGTACCA 
    TGCATTTTTC CAGATTACGA GTTTGGAACT GAGATTTTAA AATACATACG 
    TACCACCAGA TCTCCCACGG TGAGAATTAC TGCAGCTACT ACACTAACTG 
    GTCAGCCTGC AACAACTAAG GTTGCTGCAT TCTCATGTAG AGGGCCTAAT 
    TCGGTTTCAC CAGCCATTCT CAAGCCTGAT ATAGCAGCTC CGGGTGTGAG 
    CATACTCGCG GCAATAAGTC CGCTTAATCC AGAAGAACAG AACGGATTTG 
    GACTTCTTTC AGGGACATCA ATGTCAACTC CTGTTGTTTC TGGAATAATA 
    GCTCTCCTAA AATCTCTTCA CCCTAAGTGG TCTCCTGCTG CAGTGAGATC 
    AGCTTTGGTC ACAACAGCTT GGAGGACAAG TCCATCTGGA GAACCAATCT 
    TTGCTGAAGG ATCAAACAAG AAGCTTGCAG ATCCATTTGA CTATGGAGGA 
    GGACTTGTAA ACCCTGAAAA AGCTGCTAAA CCAGGACTTG TCTACGATAT 
    GGGGATTGTG GATTACATCA AGTATATGTG TTCTGCGGGT TACAACGACT 
    CTTCAATCTC TCGTGTGCTC GGTAAAAAGA CTAATTGCCC AATTCCTAAG 
    CCATCAATGC TTGATATCAA CTTACCTTCA ATCACAATTC CAAATCTTGA 
    GAAAGAAGTC ACACTCACAA GAACTGTAAC CAATGTTGGA CCCATCAAGT 
    CAGTCTATAG AGCTGTGATC GAGTCTCCTC TCGGTATAAC TCTCACCGTG 
    AACCCAACCA CACTCGTGTT TAAGTCTGCA GCTAAGAGAG TACTCACTTT 
    CTCGGTTAAG GCTAAAACAA GTCACAAAGT CAACACTGGC TACTTCTTTG 
    GAAGCTTAAC ATGGAGTGAT GGTGTTCATG ATGTTATAAT CCCTGTCTCT 
    GTTAAGACAA CGATTTCGAT GTAATAAAAA GAGCCATATG ATGCTCATTG 
    TGTTTGTTCT TATGTAATTT GTTGCAAATC TAAGTCTAAA ATGT

    Gene models with high sequence identity

    1. AT4G21650.1 [+] show detail - Subtilase family protein
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPanther SUBTILISIN-LIKE PROTEASE (PLANT) 0
    HMMPanther Peptidase S8, subtilisin-related 0
    PatternScan Peptidase S8/S53, subtilisin, active site 0
    Gene3D Peptidase S8/S53, subtilisin/kexin/sedolisin 2.9e-67
    FPrintScan Peptidase S8/S53, subtilisin/kexin/sedolisin 1.7e-12
    FPrintScan Peptidase S8/S53, subtilisin/kexin/sedolisin 1.7e-12
    FPrintScan Peptidase S8/S53, subtilisin/kexin/sedolisin 1.7e-12
    HMMPfam Peptidase S8/S53, subtilisin/kexin/sedolisin 5.6e-33
    HMMPfam Proteinase inhibitor I9, subtilisin propeptide 1.5e-21
    HMMPfam Protease-associated PA 7.6e-06
    superfamily Peptidase S8/S53, subtilisin/kexin/sedolisin 3.3e-73
    superfamily Proteinase inhibitor, propeptide 5.8e-07

    See the detailed result >

    AT4G21650.1

    Model type
    Protein coding
    Short Description
    Subtilase family protein
    Computational Description
    Subtilase family protein; FUNCTIONS IN: identical protein binding, serine-type endopeptidase activity; INVOLVED IN: proteolysis, negative regulation of catalytic activity; LOCATED IN: plant-type cell wall; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Protease-associated PA (InterPro:IPR003137), Proteinase inhibitor, propeptide (InterPro:IPR009020), Peptidase S8/S53, subtilisin/kexin/sedolisin (InterPro:IPR000209), Peptidase S8, subtilisin-related (InterPro:IPR015500), Peptidase S8/S53, subtilisin, active site (InterPro:IPR022398), Proteinase inhibitor I9, subtilisin propeptide (InterPro:IPR010259); BEST Arabidopsis thaliana protein match is: Subtilase family protein (TAIR:AT4G21630.1); Has 7770 Blast hits to 6733 proteins in 1165 species: Archae - 228; Bacteria - 4634; Metazoa - 31; Fungi - 250; Plants - 1972; Viruses - 0; Other Eukaryotes - 655 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR