F05708

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F05708

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1  
cauline leaf (PO:0000013)
  • epinastic (PATO:0000945)
    (Cauline leaf::Shape::epinastic)
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
sporophyte vegetative stage (PO:0007134)
  • decreased speed (PATO:0000304)
    (Adult plant::Growth::slow)
fruit (PO:0009001)
  • decreased length (PATO:0000574)
    (Silique::Shape::short)
root (PO:0009005)
  • present in greater numbers in organism (PATO:0000470)
    (Root::Number::many)
seed (PO:0009010)
  • present in fewer numbers in organism (PATO:0001997)
    (Seed::Number::few)
stem (PO:0009047)
  • present in greater numbers in organism (PATO:0000470)
    (Stem::Number::many)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F05708

Determination of introduced cDNA(s)

  • RAFL05-20-O23 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-20-O23 - Resource: pda07458

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-20-O23 - full reading
    ACCTTTCCCT CACCTGATAT TTATTTTCTT ACCTTAAATA CGACGGTGCT 
    TCACTGAGTC CGACTCAGTT AACTCGTTCC TCTCTCTGTG TGTGGTTTTG
    GTAGACGACG ACGACGATAA TGGAGGAGCT AGATCGTTCA CGTACTTTTG
    CCAGAGACGT CAAACGTATC GTCGTTAAGG TTGGGACAGC AGTTGTTACT
    GGAAAAGGTG GAAGATTGGC TCTTGGTCGT TTAGGAGCAC TGTGTGAACA
    GCTTGCGGAA TTAAACTCGG ATGGATTTGA GGTGATATTG GTGTCATCTG
    GTGCGGTTGG TCTTGGCAGG CAAAGGCTTC GTTATCGACA ATTAGTCAAT
    AGCAGCTTTG CGGATCTTCA GAAGCCTCAG ACTGAACTTG ATGGGAAGGC
    TTGTGCTGGT GTTGGACAAA GCAGTCTTAT GGCTTACTAT GAGACTATGT
    TTGACCAGCT TGATGTGACG GCAGCTCAAC TTCTGGTGAA TGACAGTAGT
    TTTAGAGACA AGGATTTCAG GAAGCAACTT AATGAAACTG TCAAGTCTAT
    GCTTGATTTG AGGGTTATTC CAATTTTCAA TGAGAATGAT GCTATTAGCA
    CCCGAAGAGC CCCATATCAG GATTCTTCTG GTATTTTCTG GGATAACGAT
    AGCTTAGCTG CTCTACTGGC GTTGGAACTG AAAGCTGATC TTCTGATTCT
    TCTGAGCGAT GTTGAAGGTC TTTACACAGG CCCTCCAAGT GATCCTAACT
    CAAAGTTGAT CCACACTTTT GTTAAAGAAA AACATCAAGA TGAGATTACA
    TTCGGCGACA AATCAAGATT AGGGAGAGGG GGTATGACTG CAAAAGTCAA
    AGCTGCAGTC AATGCAGCTT ATGCTGGGAT TCCTGTCATC ATAACCAGTG
    GGTATTCAGC TGAGAACATA GATAAAGTCC TCAGAGGACT ACGTGTTGGA
    ACCTTGTTTC ATCAAGATGC TCGTTTATGG GCTCCGATCA CAGATTCTAA
    TGCTCGTGAC ATGGCAGTTG CTGCGAGGGA AAGTTCCAGA AAGCTTCAGG
    CCTTATCTTC GGAAGACAGG AAAAAAATTC TGCTTGATAT TGCCGATGCC
    CTTGAAGCAA ATGTTACTAC AATCAAAGCT GAGAATGAGT TAGATGTAGC
    TTCTGCACAA GAGGCTGGGT TGGAAGAGTC AATGGTGGCT CGCTTAGTTA
    TGACACCTGG AAAGATCTCG AGCCTTGCAG CTTCAGTTCG TAAGCTAGCT
    GATATGGAAG ATCCAATCGG CCGTGTTTTA AAGAAAACAG AGGTGGCAGA
    TGGTCTTGTC TTAGAGAAGA CCTCATCACC ATTAGGCGTA CTTCTGATTG
    TTTTTGAATC CCGACCTGAT GCACTTGTAC AGATAGCTTC ACTTGCCATC
    CGTAGTGGAA ATGGTCTTCT GCTGAAGGGT GGAAAGGAGG CCCGGCGATC
    AAATGCTATC TTACACAAGG TGATCACTGA TGCAATTCCA GAGACTGTTG
    GGGGTAAACT CATTGGACTT GTGACTTCAA GAGAAGAGAT TCCTGATTTG
    CTTAAGCTTG ATGACGTTAT CGATCTTGTG ATCCCAAGAG GAAGCAACAA
    GCTTGTTACT CAGATAAAAA ATACTACAAA AATCCCTGTG CTAGGTCATG
    CTGATGGAAT CTGTCATGTA TATGTCGACA AGGCTTGTGA TACGGATATG
    GCAAAGCGCA TAGTTTCTGA TGCAAAGTTG GACTATCCAG CAGCCTGTAA
    TGCGATGGAA ACCCTTCTTG TGCATAAGGA TCTAGAGCAG AATGCTGTGC
    TTAATGAGCT TATTTTTGCT CTGCAGAGCA ATGGAGTCAC TTTGTATGGT
    GGACCAAGGG CAAGTAAGAT ACTGAACATA CCAGAAGCAC GGTCATTCAA
    CCATGAGTAC TGTGCCAAGG CTTGCACTGT TGAAGTTGTA GAAGACGTTT
    ATGGTGCTAT AGATCACATT CACCGACATG GGAGTGCACA CACAGACTGC
    ATTGTGACAG AGGATCACGA AGTTGCAGAG CTATTCCTTC GCCAAGTGGA
    TAGCGCTGCT GTGTTCCACA ACGCCAGCAC AAGATTCTCA GATGGTTTCC
    GATTTGGACT TGGTGCAGAG GTGGGGGTAA GCACGGGCAG GATCCATGCT
    CGTGGTCCAG TCGGGGTCGA AGGATTACTT ACAACGAGAT GGATAATGAG
    AGGAAAAGGA CAAGTTGTCG ACGGAGACAA TGGAATTGTT TACACCCATC
    AGGACATTCC CATCCAAGCT TAAACAAGAC TTCCGAGTGT GTGTTTGTGT
    ATTTGGTTGA GACTTGAGGA GAGACACAGA GGAGGATGGG CTTTTTTGTT
    TCCTCTCTGC TTAGTACTCA TATCCTATCA TTATTATTAT TACTACTACT
    TATTATTGAA ACCCTCGCTT ATGTAGTGGT TTTGATTTAG GGTTAGGATT
    GCACCAAAAA TAAGATCCAC TTTACCACTT AGTCTTGCTC ATAAGTACGA
    TGAAGAACAT TTAATTAGCT TCTCTTCTTG TCATTGTAAG CTACCTACAC
    ATTTCTGATC TTTATCAAGA TACTACTACT TTTCAAAAAA AAAAAAAAAA

    Gene models with high sequence identity

    1. AT2G39800.1 [+] show detail - delta1-pyrroline-5-carboxylate synthase 1
      E-value: 0; Score: 99.96

     

    InterPro Scan Digest

    Program Description E-value
    PatternScan Glutamate 5-kinase, conserved site 0
    PatternScan Gamma-glutamyl phosphate reductase GPR, conserved site 0
    HMMPanther DELTA 1-PYRROLINE-5-CARBOXYLATE SYNTHETASE 0
    HMMPanther GLUTAMATE SEMIALDEHYDE DEHYDROGENASE 0
    HMMPfam Aspartate/glutamate/uridylate kinase 3.9e-37
    HMMPfam Aldehyde dehydrogenase 0.000000069
    HMMTigr Delta l-pyrroline-5-carboxylate synthetase 0
    HMMTigr Glutamate 5-kinase, ProB-related 7e-26
    HMMTigr Gamma-glutamyl phosphate reductase GPR 8.9e-161
    Gene3D Aspartate/glutamate/uridylate kinase 9.9e-18
    Gene3D Aldehyde dehydrogenase, N-terminal 5.9e-56
    Gene3D Aldehyde dehydrogenase, C-terminal 3.6e-64
    superfamily Aldehyde/histidinol dehydrogenase 4.9e-99
    superfamily Aspartate/glutamate/uridylate kinase 3.7e-53
    HMMPIR Delta l-pyrroline-5-carboxylate synthetase 0
    FPrintScan Glutamate 5-kinase 3.4e-56
    FPrintScan Glutamate 5-kinase 3.4e-56
    FPrintScan Glutamate 5-kinase 3.4e-56
    FPrintScan Glutamate 5-kinase 3.4e-56
    FPrintScan Glutamate 5-kinase 3.4e-56

    See the detailed result >

    AT2G39800.1

    Model type
    Protein coding
    Short Description
    delta1-pyrroline-5-carboxylate synthase 1
    Curator Summary
    encodes a delta1-pyrroline-5-carboxylate synthase that catalyzes the rate-limiting enzyme in the biosynthesis of proline. Gene is expressed in reproductive organs and tissues under non-stress conditions but in the whole plant under water-limiting condition. Expression is also induced by abscisic acid and salt stress in a light-dependent manner. P5CS1 appears to be involved in salt stress responses related to proline accumulation, including protection from reactive oxidative species. P5CS1 appears to be present in different cells and/or different subcellular locations from P5CS2 in a tissue-dependent manner.
    Computational Description
    delta1-pyrroline-5-carboxylate synthase 1 (P5CS1); FUNCTIONS IN: delta1-pyrroline-5-carboxylate synthetase activity; INVOLVED IN: in 9 processes; LOCATED IN: chloroplast, membrane, cytoplasm; EXPRESSED IN: 6 plant structures; EXPRESSED DURING: C globular stage, D bilateral stage, E expanded cotyledon stage; CONTAINS InterPro DOMAIN/s: Glutamate 5-kinase (InterPro:IPR001057), Aspartate/glutamate/uridylate kinase (InterPro:IPR001048), Glutamate 5-kinase, conserved site (InterPro:IPR019797), Aldehyde dehydrogenase, N-terminal (InterPro:IPR016162), Gamma-glutamyl phosphate reductase GPR, conserved site (InterPro:IPR020593), Aldehyde dehydrogenase, C-terminal (InterPro:IPR016163), Aldehyde/histidinol dehydrogenase (InterPro:IPR016161), Delta l-pyrroline-5-carboxylate synthetase (InterPro:IPR005766), Gamma-glutamyl phosphate reductase GPR (InterPro:IPR000965), Aldehyde dehydrogenase (InterPro:IPR015590), Glutamate 5-kinase, ProB-related (InterPro:IPR005715); BEST Arabidopsis thaliana protein match is: delta 1-pyrroline-5-carboxylate synthase 2 (TAIR:AT3G55610.1); Has 14932 Blast hits to 14924 proteins in 2380 species: Archae - 163; Bacteria - 9254; Metazoa - 175; Fungi - 266; Plants - 182; Viruses - 0; Other Eukaryotes - 4892 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR