F05922

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F05922

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1  
sporophyte vegetative stage (PO:0007134)
  • decreased speed (PATO:0000304)
    (Adult plant::Growth::slow)
root (PO:0009005)
  • present in greater numbers in organism (PATO:0000470)
    (Root::Number::many)
T2  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
cauline leaf (PO:0000013)
  • decreased width (PATO:0000599)
    (Cauline leaf::Shape::narrow)
  • epinastic (PATO:0000945)
    (Cauline leaf::Shape::epinastic)
  • desaturated green (PATO:0001272)
    (Cauline leaf::Color::pale)
rosette leaf (PO:0000014)
  • desaturated green (PATO:0001272)
    (Rosette leaf::Color::pale)
  • epinastic (PATO:0000945)
    (Rosette leaf::Shape::epinastic)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
  • present in fewer numbers in organism (PATO:0001997)
    (Rosette leaf::Number::few)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
stem (PO:0009047)
  • desaturated green (PATO:0001272)
    (Stem::Color::pale)
  • abnormal (PATO:0000460)
    (Stem::Others)
  • present in greater numbers in organism (PATO:0000470)
    (Stem::Number::many)
  • abnormal (PATO:0000460)
    (Stem::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F05922

Determination of introduced cDNA(s)

  • RAFL05-21-C12 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-21-C12 - Resource: pda01322

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-21-C12 - full reading
    AACACACGCC TCTAATCTCC GCGCACACAC ACACACCCTC ACGAAGAAGA 
    AGAAGACGAA GAAGAATCAA TGGCTTCTGT TATCTCTTCC TCTCCTTTTC
    TATGCAAATC ATCCTCCAAG AGTGATTTGG GGATTTCTTC GTTTCCTAAA
    TCTTCTCAGA TTTCGATTCA TCGATGTCAG AAGAAATCGA TCTCACGGAA
    GATTGTTTCC GTTATGGCTC CTCAAAAGGA TCGGTCTCCT GGAACTACAG
    GATCGGTGAA AACTGGGATG ACAATGACGG AGAAGATTCT AGCTAGAGCT
    TCGGAGAAGT CATTAGTGGT TCCTGGTGAT AACATTTGGG TTAATGTTGA
    TGTTCTTATG ACTCATGATG TTTGTGGTCC TGGTGCTTTT GGTATCTTCA
    AGAGAGAGTT TGGTGAAAAA GCCAAGGTTT GGGATCCGGA GAAGATTGTT
    GTGATTCCGG ATCATTATAT TTTCACTGCT GATAAGCGTG CAAACCGGAA
    TGTTGACATC ATGAGGGAGC ATTGCAGGGA ACAGAATATT AAGTATTTCT
    ATGATATCAC TGACCTTGGA AATTTTAAGG CTAATCCTGA CTACAAAGGT
    GTTTGCCATG TTGCACTTGC ACAAGAAGGT CATTGCAGGC CAGGAGAGGT
    TTTGTTAGGA ACAGACTCAC ACACTTGTAC TGCTGGAGCA TTTGGTCAAT
    TTGCTACAGG GATTGGAAAC ACTGATGCAG GTTTTGTGTT AGGCACTGGA
    AAAATCCTCC TTAAGGTTCC ACCAACGATG AGGTTTATCT TGGATGGTGA
    GATGCCTAGT TATTTGCAAG CAAAGGATCT GATTTTACAA ATTATTGGTG
    AAATATCTGT TGCTGGTGCA ACTTACAAGA CGATGGAGTT CAGTGGTACA
    ACTATCGAAA GTCTGAGTAT GGAAGAAAGA ATGACATTGT GCAACATGGT
    TGTGGAAGCT GGGGGAAAGA ATGGTGTCAT CCCTCCTGAT GCGACGACAT
    TAAATTATGT TGAGAATAGG ACATCTGTAC CATTTGAGCC AGTATATAGT
    GATGGAAATG CAAGCTTTGT CGCAGATTAT AGATTTGACG TGTCAAAGCT
    GGAGCCTGTG GTGGCTAAGC CTCATTCTCC TGACAACCGA GCTCTAGCTA
    GAGAATGCAA AGACGTGAAA ATTGACAGAG TATACATCGG TTCTTGTACT
    GGTGGGAAGA CAGAGGATTT TATGGCTGCA GCTAAACTTT TCCACGCAGC
    AGGAAGAAAG GTCAAAGTTC CAACTTTCCT TGTCCCGGCT ACTCAGAAGG
    TGTGGATGGA TGTGTATGCT CTCCCGGTAC CTGGAGCAGG TGGAAAGACA
    TGTGCGCAGA TCTTTGAAGA AGCTGGCTGT GACACACCAG CCAGCCCTAG
    CTGTGGTGCT TGCCTTGGTG GCCCAGCAGA CACCTACGCT CGCTTGAATG
    AACCTCAAGT GTGTGTCTCA ACGACAAACA GGAACTTCCC CGGTCGGATG
    GGACACAAAG AAGGGCAAAT TTACTTAGCG TCTCCTTACA CTGCTGCAGC
    CTCGGCTTTA ACCGGCCGTG TCGCTGACCC AAGGGAGTTC TTGCAGTAGG
    CTAATGTAAT GGCTTCGTGT GAAAAATCAT ATGCTATGCA TCGACGAAAT
    ATTGCTAAAT GATGGGGCAT TGTGAACTAT ATGTATGTAT TTTCCTTATT
    TCGCTGTTGG ACTTGATAGT TTCAAGCACT ATGTCAAACT TTGATGTTTT
    GAGTTTGAAA TAATAAAAGA GAGAAAAAGA GTGAGTTAGA TTAAAAAAAA
    AAAAAAAA

    Gene models with high sequence identity

    1. AT4G13430.1 [+] show detail - isopropyl malate isomerase large subunit 1
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    FPrintScan Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha 9.4e-17
    FPrintScan Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha 9.4e-17
    FPrintScan Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha 9.4e-17
    FPrintScan Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha 9.4e-17
    FPrintScan Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha 9.4e-17
    HMMPanther Homoaconitase/3-isopropylmalate dehydratase, small/large subunit 5.9e-194
    HMMPanther Aconitase-like core 5.9e-194
    Gene3D Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 1/3 1.7e-28
    Gene3D Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 2 3.5e-28
    Gene3D Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 1/3 2.9e-39
    HMMTigr Homoaconitase/3-isopropylmalate dehydratase, large subunit, subgroup 2e-143
    superfamily Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha 2.3e-124
    HMMPfam Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha 7.9e-53
    HMMPfam Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha 2.2e-19

    See the detailed result >

    AT4G13430.1

    Model type
    Protein coding
    Short Description
    isopropyl malate isomerase large subunit 1
    Curator Summary
    Encodes methylthioalkylmalate isomerase.
    Computational Description
    isopropyl malate isomerase large subunit 1 (IIL1); FUNCTIONS IN: lyase activity, intramolecular transferase activity, transferring hydroxy groups; INVOLVED IN: glucosinolate biosynthetic process, response to cadmium ion, metabolic process; LOCATED IN: chloroplast stroma, chloroplast; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha (InterPro:IPR001030), Homoaconitase/3-isopropylmalate dehydratase, small/large subunit (InterPro:IPR015936), Homoaconitase/3-isopropylmalate dehydratase, large subunit, subgroup (InterPro:IPR006251), Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 2 (InterPro:IPR015932), Aconitase-like core (InterPro:IPR015937), Aconitase/3-isopropylmalate dehydratase large subunit, alpha/beta/alpha, subdomain 1/3 (InterPro:IPR015931); BEST Arabidopsis thaliana protein match is: aconitase 2 (TAIR:AT4G26970.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR