F06042

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F06042

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
cauline leaf (PO:0000013)
  • decreased size (PATO:0000587)
    (Cauline leaf::Shape::small)
  • present in fewer numbers in organism (PATO:0001997)
    (Cauline leaf::Number::few)
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • present in fewer numbers in organism (PATO:0001997)
    (Rosette leaf::Number::few)
sporophyte vegetative stage (PO:0007134)
  • decreased speed (PATO:0000304)
    (Adult plant::Growth::slow)
seed (PO:0009010)
  • present in fewer numbers in organism (PATO:0001997)
    (Seed::Number::few)
stem (PO:0009047)
  • decreased thickness (PATO:0000592)
    (Stem::Shape::thin)
  • abnormal (PATO:0000460)
    (Stem::Shape::zigzag)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F06042

Determination of introduced cDNA(s)

  • RAFL09-43-F09 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL09-43-F09 - Resource: pda09781

    full reading [+] show sequence

    Nucleotide sequence: RAFL09-43-F09 - full reading
    AAAAAGAAGT CTTCTTCCAT AGTTACTAGT TAGAGCTGCG AAACGAAAAT 
    CGTAACGATA GTGCATCAAT GGCTTATGCG TCACGCATCA TTAATCATTC
    CAAGAAGTTG AAGGATGTTT CCACTTTACT ACGCCGTGAG AATGCTGCAA
    CGATCCGCTA CTACTCGAAT ACTAATCGAG CTCCTCTGAA CAGAGAAGAT
    ACTTTCAATT CCCGTCTTGG TTATCCCCCA CTGGAGAGGA TTTCCATATG
    TAGCACCAGC ACTTTACCGG TGTCCATTAT TTTTTCAACT ACGAGATCAA
    ATCTAAGCAG TGCAATGGGA AGGCCAATAT TTGGAAAAGA ATTTTCATGT
    TTGATGCAAT CAGCTAGAGG ATTTTCATCG GGTTCAGATC TGCCTCCTCA
    TCAAGAGATC GGGATGCCTT CTCTCTCGCC AACAATGACT GAGGGTAACA
    TCGCCAGGTG GCTGAAAAAA GAAGGTGATA AAGTTGCTCC TGGGGAAGTA
    CTCTGTGAAG TTGAAACTGA CAAAGCAACT GTCGAAATGG AATGCATGGA
    AGAGGGCTAT CTCGCCAAGA TTGTAAAGGC GGAAGGTTCA AAAGAAATTC
    AAGTCGGCGA GGTAATTGCT ATTACAGTTG AAGATGAAGA GGACATTGGA
    AAGTTCAAAG ATTACACCCC ATCATCTACT GCTGATGCCG CTCCTACAAA
    AGCAGAACCA ACTCCTGCCC CACCAAAGGA AGAGAAGGTC AAACAGCCAT
    CATCGCCTCC TGAACCGAAG GCCTCCAAGC CTAGCACACC TCCCACAGGA
    GATCGTGTTT TTGCTAGTCC TCTTGCAAGA AAGTTGGCTG AAGATAATAA
    CGTGCCTCTC TCAGACATCG AAGGCACAGG TCCTGAAGGA CGGATAGTGA
    AGGCAGATAT CGATGAGTAT TTAGCTTCAA GTGGTAAAGG AGCTACTGCG
    AAGCCATCTA AGTCAACTGA TTCTAAGGCC CCAGCTTTGG ACTATGTTGA
    CATCCCTCAT TCTCAGATAC GAAAGGTCAC AGCCTCACGT TTGGCATTCT
    CAAAGCAAAC TATCCCTCAC TATTACTTAA CCGTCGATAC ATGTGTTGAC
    AAACTTATGG CTCTGAGGAG TCAGCTTAAT TCATTCAAAG AGGCTTCTGG
    TGGGAAACGG ATATCTGTCA ATGATCTTGT TGTTAAGGCT GCTGCATTAG
    CTCTCAGAAA AGTTCCTCAG TGCAATAGTT CATGGACTGA CGATTACATC
    CGCCAGTTTA AAAATGTGAA CATCAACGTC GCGGTGCAGA CAGAAAACGG
    GCTGTATGTC CCTGTTGTGA AGGACGCAGA CAGGAAGGGT CTGTCCACAA
    TTGGAGAAGA AGTTCGGTTA TTAGCTCAAA AAGCAAAAGA GAGCAGCTTA
    AAGCCAGAAG ATTATGAGGG AGGAACATTT ACTGTCTCTA ACTTGGGAGG
    ACCTTTTGGC ATCAAGCAAT TCTGTGCAGT CGTTAATCCA CCTCAAGCCG
    CCATTCTAGC AGTTGGATCC GCTGAAAAGA GAGTTGTCCC TGGTAACGGT
    CCAGATCAAT TCAACTTTGC TTCTTACATG CCTGTTACAC TGAGCTGTGA
    CCATCGCGTA GTAGACGGTG CCATTGGAGC TGAATGGTTG AAAGCATTTA
    AGGGATACAT CGAGAACCCT AAATCTATGT TACTCTAAAA GAGAACCAAC
    CACGCAATGG AAAAAAACAC ATCAAAAGCT GTCGTAAGAG TCTCTGTACC
    AATTAGATTT TCTTTGTTTT CCTCTGTTTC TTTCGGGTTT TTCTAAAAAA
    GACTTTTGTT GTTTTCTGAA TAAATATGGT GTGAGGGATT CAGCATTTCC
    ACTGTAAACT GGATTTTAGG ATATTCTTTG CTGGTTCTGG TTGTTCAATG
    TTCATTGAGG AGGGCTGTGA AATAAAACTC CCATTATTTT GTCATGTGAA
    GTACTACCCT ATATGGGATG GGTTTTGTAA CCATGCTTGA TAGTTGATAC
    AAGTGGATGG ATTTGAGT

    Gene models with high sequence identity

    1. AT1G54220.1 [+] show detail - Dihydrolipoamide acetyltransferase, long form protein
      E-value: 0; Score: 99.95

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam 2-oxoacid dehydrogenase acyltransferase, catalytic domain 7e-78
    HMMPfam Biotin/lipoyl attachment 1.2e-19
    HMMPfam E3 binding 3.2e-16
    ProfileScan Biotin/lipoyl attachment 15.443
    HMMPanther DIHYDROLIPOAMIDE ACETYLTRANSFERASE COMPONENT 1.4e-296
    HMMPanther DIHYDROLIPOAMIDE ACETYL/SUCCINYL-TRANSFERASE- 1.4e-296
    Gene3D no description 9.3e-32
    Gene3D E3 binding 4.8e-07
    Gene3D no description 5.7e-73
    HMMTigr Dihydrolipoamide acetyltransferase, long form 2.1e-210
    superfamily CoA-dependent acyltransferases 1.7e-79
    superfamily Single hybrid motif 5.4e-30
    superfamily E3 binding 5.5e-13
    PatternScan 2-oxo acid dehydrogenase, lipoyl-binding site 0

    See the detailed result >

    AT1G54220.1

    Model type
    Protein coding
    Short Description
    Dihydrolipoamide acetyltransferase, long form protein
    Computational Description
    Dihydrolipoamide acetyltransferase, long form protein; FUNCTIONS IN: dihydrolipoyllysine-residue acetyltransferase activity, acyltransferase activity; INVOLVED IN: pyruvate metabolic process, metabolic process, acetyl-CoA biosynthetic process from pyruvate; LOCATED IN: mitochondrion; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: 2-oxo acid dehydrogenase, lipoyl-binding site (InterPro:IPR003016), Dihydrolipoamide acetyltransferase, long form (InterPro:IPR006257), E3 binding (InterPro:IPR004167), 2-oxoacid dehydrogenase acyltransferase, catalytic domain (InterPro:IPR001078), Single hybrid motif (InterPro:IPR011053), Biotin/lipoyl attachment (InterPro:IPR000089); BEST Arabidopsis thaliana protein match is: Dihydrolipoamide acetyltransferase, long form protein (TAIR:AT3G13930.1); Has 21519 Blast hits to 19723 proteins in 2310 species: Archae - 104; Bacteria - 12081; Metazoa - 737; Fungi - 478; Plants - 377; Viruses - 0; Other Eukaryotes - 7742 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR