F07931

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F07931

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F07931

Determination of introduced cDNA(s)

  • RAFL05-15-L01 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-15-L01 - Resource: pda07330

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-15-L01 - full reading
    ACTGGCCCAA GTCATTCAAC AACTTGGCTT TTATCTAATA AAGCCCCTCC 
    CTCTCCGATA AACCTTAAAT AAAACCGGAG GGAGAGCAAA AAATAAAAAC
    AAAAATATAC AAAAACAAAA ACAAAAAATC ATAAAAAATG ATTGGAAAAG
    TTGTGGTCTC GGTGGCCTCC ATCCTCTTAA TAGTTGGAGT TGCCATAGGA
    GTCGTTGCCT ACATAAACAA AAATGGTGAT GCTAATCTGT CTCCCCAAAT
    GAAAGCGGTC CGAGGAATTT GCGAGGCGAC TTCCGACAAA GCCTCATGTG
    TCAAAACTCT CGAGCCGGTC AAGAGCGATG ACCCAAACAA GCTGATCAAG
    GCTTTCATGC TCGCTACCAG AGATGCTATA ACCCAATCAT CAAACTTCAC
    GGGTAAAACC GAAGGAAACT TGGGTTCGGG CATCTCGCCA AACAACAAAG
    CCGTTCTTGA TTACTGCAAG AAAGTTTTCA TGTACGCGCT TGAGGATCTC
    AGCACCATTG TTGAGGAAAT GGGTGAAGAT CTTAACCAGA TCGGGAGCAA
    AATCGACCAG CTTAAACAAT GGTTAACCGG TGTTTACAAT TACCAAACTG
    ATTGTCTTGA CGATATCGAA GAAGATGATT TAAGAAAGAC TATTGGAGAG
    GGCATTGCAA GCTCCAAGAT TCTCACTAGC AACGCTATTG ACATCTTCCA
    CACTGTCGTT AGCGCCATGG CCAAGCTTAA CTTGAAGGTC GAGGACTTCA
    AGAACATGAC AGGCGGAATC TTCGCTCCTT CAGACAAAGG AGCAGCTCCC
    GTCAACAAAG GAACCCCTCC TGTTGCTGAT GATTCTCCCG TGGCCGATCC
    AGATGGTCCT GCTCGCCGTC TTCTTGAAGA CATTGACGAG ACCGGAATCC
    CAACATGGGT TTCAGGTGCT GACAGGAAGC TCATGACTAA GGCTGGACGT
    GGCAGTAATG ACGGTGGTGC TAGGATCAGA GCAACCTTTG TGGTGGCTAA
    GGACGGAAGT GGACAGTTTA AGACTGTTCA ACAAGCCGTT AATGCTTGTC
    CTGAGAAAAA CCCAGGCCGT TGCATCATCC ACATCAAGGC TGGTATCTAC
    AGAGAGCAAG TGATCATCCC TAAGAAGAAG AACAACATCT TCATGTTCGG
    AGATGGTGCA AGAAAGACCG TCATTTCTTA CAACAGAAGT GTTAAACTCA
    GCCCTGGAAC CACCACTTCC CTTAGTGGCA CAGTCCAGGT TGAGTCCGAA
    GGATTCATGG CAAAATGGAT CGGATTCAAG AACACCGCCG GTCCAATGGG
    ACACCAAGCT GTGGCTATCA GAGTGAACGG AGACCGTGCC GTGATCTTCA
    ACTGCAGGTT CGACGGTTAC CAAGACACCT TGTACGTCAA CAATGGTCGT
    CAGTTCTACA GAAACATTGT CGTGTCAGGA ACAGTCGACT TCATCTTCGG
    CAAATCCGCA ACCGTGATCC AAAACTCACT CATTGTTGTC CGTAAAGGAA
    ACAAGGGACA ATTCAACACG GTCACAGCCG ATGGAAACGA AAAGGGTCTA
    GCGATGAAAA TCGGTATCGT CCTCCAAAAC TGTCGCATCG TTCCCGACAA
    GAAACTAGCG GCAGAGAGAT TAATCGTGGA GTCATACTTG GGAAGGCCAT
    GGAAGAAATT CTCTACCACC GTGATTATCA ACTCCGAGAT AGGAGATGTG
    ATTAGACCAG AAGGTTGGAA GATTTGGGAT GGGGAAAGTT TCCACAAGTC
    ATGTAGGTAC GTTGAGTACA ACAACCGTGG ACCAGGAGCT ATCACTAACC
    GAAGAGTTAA CTGGGTTAAG ATCGCCAGGT CTGCAGCTGA GGTCAATGAC
    TTCACTGTTG CTAACTGGTT AGGTCCTATT AACTGGATTC AAGAAGCCAA
    TGTTCCCGTC ACGCTTGGAT TATAAGGCGT ATAAATCAAA TCAAATCAAA
    CC

    Gene models with high sequence identity

    1. AT2G47040.1 [+] show detail - Plant invertase/pectin methylesterase inhibitor superfamily
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Pectinesterase, catalytic 1.5e-111
    HMMPfam Pectinesterase inhibitor 7.2e-20
    HMMSmart Pectinesterase inhibitor 1.7e-32
    HMMTigr Pectinesterase inhibitor 3.1e-05
    superfamily Pectin lyase fold/virulence factor 2.3e-97
    superfamily Pectinesterase inhibitor 1.4e-16
    PatternScan Pectinesterase, active site 0
    PatternScan Pectinesterase, active site 0
    Gene3D Pectinesterase inhibitor 1.7e-05
    Gene3D Pectin lyase fold 6.6e-110

    See the detailed result >

    AT2G47040.1

    Model type
    Protein coding
    Short Description
    Plant invertase/pectin methylesterase inhibitor superfamily
    Curator Summary
    Share high homologies with a group of pectin methylesterases (PME), pollen specific, and is required for enhancing the growth of pollen tube in style and transmitting tract tissues.
    Computational Description
    VANGUARD1 (VGD1); FUNCTIONS IN: enzyme inhibitor activity, pectinesterase activity; INVOLVED IN: pollen tube growth; LOCATED IN: endomembrane system, cell wall, plant-type cell wall; EXPRESSED IN: male gametophyte, flower, pollen tube; EXPRESSED DURING: L mature pollen stage, M germinated pollen stage; CONTAINS InterPro DOMAIN/s: Pectinesterase, active site (InterPro:IPR018040), Pectin lyase fold/virulence factor (InterPro:IPR011050), Pectinesterase, catalytic (InterPro:IPR000070), Pectinesterase inhibitor (InterPro:IPR006501), Pectin lyase fold (InterPro:IPR012334); BEST Arabidopsis thaliana protein match is: Plant invertase/pectin methylesterase inhibitor superfamily (TAIR:AT2G47030.1); Has 2977 Blast hits to 2908 proteins in 464 species: Archae - 6; Bacteria - 891; Metazoa - 1; Fungi - 197; Plants - 1856; Viruses - 0; Other Eukaryotes - 26 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR