F09409

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F09409

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • increased size (PATO:0000586)
    (Seed::Shape::large)
  • increased length (PATO:0000573)
    (Seed::Shape::long)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F09409

Determination of introduced cDNA(s)

  • RAFL04-10-F12 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL04-10-F12 - Resource: pda00289

    full reading [+] show sequence

    Nucleotide sequence: RAFL04-10-F12 - full reading 
    AGAGCTAACC CCGGAGAGCT TACAGAATTC AAGCTAAACA TTGTTAGAGA 
    GAAAGAGAGA GCGATAATGG CGATGAGGGG CTTCACGTTG TTTTCGATCC 
    TTGTGTTGTC TTTGTGCGCT TCGTCTATCA GAAGCGAAGA GACGGAGACG 
    AAGGAGTTCG TGTTGACCTT GGATCACACT AACTTCACCG ATACCATCAA 
    CAAACACGAT TTCATCGTCG TCGAGTTCTA CGCCCCATGG TGTGGACACT 
    GCAAGCAGCT TGCTCCTGAG TATGAGAAGG CTGCGTCAGC TTTGAGCAGT 
    AACGTCCCAC CAGTGGTTCT TGCTAAGATT GATGCCAGTG AGGAAACAAA 
    CAGAGAATTT GCAACTCAAT ACGAGGTTCA GGGTTTCCCA ACAATCAAGA 
    TTTTCAGAAA CGGAGGAAAG GCTGTTCAAG AATACAACGG ACCTCGTGAA 
    GCTGAGGGTA TTGTTACTTA CTTGAAGAAA CAAAGTGGAC CTGCTTCTGC 
    TGAAATTAAG TCAGCTGATG ATGCTTCTGA GGTTGTTAGT GACAAGAAGG 
    TTGTTGTGGT TGGGATTTTC CCTAAACTAT CTGGCTCCGA GTTTGATTCT 
    TTCATGGCCA TTGCTGAGAA ATTGCGCTCT GAGTTAGATT TCGCACATAC 
    CTCGGATGCC AAGCTTCTTC CCCGTGGAGA GTCATCTGTA ACAGGACCTG 
    TGGTCAGGCT ATTCAAACCC TTTGATGAAC AATTTGTTGA TTCCAAGGAT 
    TTCGATGGTG AAGCTCTGGA GAAATTTGTC AAAGAATCCA GCATTCCACT 
    TATCACCGTC TTTGACAAAG ATCCAAACAA CCACCCATAT GTTATCAAGT 
    TCTTTGAAAG CACTAATACC AAGGCGATGT TGTTCATTAA CTTCACTGGA 
    GAAGGAGCTG AGTCTCTTAA ATCAAAGTAC CGTGAAGTTG CTACATCCAA 
    CAAGGGACAG GGTCTTAGCT TCCTTCTAGG TGATGCTGAG AACAGCCAAG 
    GTGCATTCCA GTACTTTGGA CTCGAAGAGA GCCAAGTTCC TCTCATCATC 
    ATCCAGACTG CTGACGACAA GAAATACCTG AAAACAAATG TTGAGGTTGA 
    CCAGATTGAA TCATGGGTCA AGGACTTCAA GGATGGAAAA ATTGCTCCCC 
    ACAAAAAATC TCAACCTATC CCAGCCGAAA ACAACGAGCC AGTGAAGGTT 
    GTTGTTTCTG ACAGCCTTGA CGACATTGTC TTAAACTCTG GAAAGAACGT 
    TTTGCTTGAA TTCTATGCTC CATGGTGTGG ACACTGCCAA AAGCTTGCTC 
    CAATCTTGGA CGAAGTTGCT GTGTCGTACC AAAGCGACTC AAGTGTAGTC 
    ATCGCTAAGC TAGATGCAAC CGCAAACGAC TTCCCAAAAG ATACCTTTGA 
    TGTGAAGGGA TTCCCGACCA TTTACTTCAA ATCAGCGAGC GGAAACGTTG 
    TGGTTTACGA AGGAGACAGG ACAAAGGAAG ATTTCATAAG CTTCGTCGAC 
    AAGAACAAGG ACACAGTTGG AGAGCCTAAG AAGGAGGAAG AGACAACTGA 
    GGAAGTCAAG GATGAGCTCT GAAAAGAGAA GTGTGTTGTG TCTCTTAGCT 
    GCTAGCTTAG TTTGGTAGTT TTGAGGAAAA TACACAGAAG AAGACAGAGC 
    ATTGAGTAGA CTGAGAGACA GAGAGAGAGA GACGACAGAC AGACAGAGAG 
    AATCTCTTTA TCTATTCATT AGGTTTTTAT ATGTGCACTC TTCAGAACAA 
    TAATGGCTGC CTCCTTTTCT TTTTTTTGGT TGATGTTACC TTTTTTTGTT 
    TGAATTCAGT TCTCCTTTAA TATAGTTTTT GAGCCAAAAA AAAAAAAAAA

    Gene models with high sequence identity

    1. AT1G21750.1 [+] show detail - PDI-like 1-1
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    ProfileScan Thioredoxin-like 15.467
    ProfileScan Thioredoxin-like 16.619
    PatternScan Thioredoxin, conserved site 0
    PatternScan Thioredoxin, conserved site 0
    HMMPanther PROTEIN DISULFIDE ISOMERASE 3.9e-113
    HMMTigr Protein disulphide isomerase 4.8e-199
    HMMTigr Disulphide isomerase 2.3e-49
    HMMTigr Disulphide isomerase 1.6e-40
    FPrintScan Thioredoxin-like subdomain 1.3e-07
    FPrintScan Thioredoxin-like subdomain 1.3e-07
    FPrintScan Thioredoxin-like subdomain 1.3e-07
    HMMPfam Thioredoxin domain 7.4e-32
    HMMPfam Thioredoxin domain 5.6e-27
    Gene3D Thioredoxin fold 1.2e-32
    Gene3D Thioredoxin fold 2.1e-14
    Gene3D Thioredoxin fold 1.8e-27
    superfamily Thioredoxin-like fold 2.4e-34
    superfamily Thioredoxin-like fold 1.3e-31
    superfamily Thioredoxin-like fold 2e-20
    superfamily Thioredoxin-like fold 9.2e-14

    See the detailed result >

    AT1G21750.1

    Model type
    Protein coding
    Short Description
    PDI-like 1-1
    Curator Summary
    Encodes a protein disulfide isomerase-like (PDIL) protein, a member of a multigene family within the thioredoxin (TRX) superfamily; isoform contains non-consensus GA donor splice site at intron 9. Transcript levels for this gene are up-regulated in response to three different chemical inducers of ER stress (dithiothreitol, beta-mercaptoethanol, and tunicamycin). Neither AtIRE1-2 nor AtbZIP60 appear to be required for this response.
    Computational Description
    PDI-like 1-1 (PDIL1-1); FUNCTIONS IN: protein disulfide isomerase activity; INVOLVED IN: in 6 processes; LOCATED IN: in 8 components; EXPRESSED IN: 29 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: Disulphide isomerase (InterPro:IPR005788), Thioredoxin fold (InterPro:IPR012335), Thioredoxin domain (InterPro:IPR013766), Thioredoxin, conserved site (InterPro:IPR017937), Thioredoxin-like subdomain (InterPro:IPR006662), Protein disulphide isomerase (InterPro:IPR005792), Thioredoxin-like (InterPro:IPR017936), Thioredoxin-like fold (InterPro:IPR012336); BEST Arabidopsis thaliana protein match is: PDI-like 1-2 (TAIR:AT1G77510.1); Has 31573 Blast hits to 17834 proteins in 2924 species: Archae - 337; Bacteria - 15428; Metazoa - 5451; Fungi - 1644; Plants - 2422; Viruses - 29; Other Eukaryotes - 6262 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR