F10008

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F10008

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
whole plant (PO:0000003)
  • increased height (PATO:0000570)
    (Adult plant::Plant height::tall)
cauline leaf (PO:0000013)
  • increased size (PATO:0000586)
    (Cauline leaf::Shape::large)
rosette leaf (PO:0000014)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)
  • present in greater numbers in organism (PATO:0000470)
    (Rosette leaf::Number::many)
fruit (PO:0009001)
  • decreased length (PATO:0000574)
    (Silique::Shape::short)
root (PO:0009005)
  • present in greater numbers in organism (PATO:0000470)
    (Root::Number::many)
seed (PO:0009010)
  • present in fewer numbers in organism (PATO:0001997)
    (Seed::Number::few)
stem (PO:0009047)
  • increased thickness (PATO:0000591)
    (Stem::Shape::thick)
  • dark green (PATO:0001249)
    (Stem::Color::dark)
  • abnormal (PATO:0000460)
    (Stem::Others)
stem internode (PO:0020142)
  • decreased length (PATO:0000574)
    (Stem::Shape::short internode)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F10008

Determination of introduced cDNA(s)

  • RAFL03-09-N15 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL03-09-N15 - Resource: pda00260

    full reading [+] show sequence

    Nucleotide sequence: RAFL03-09-N15 - full reading 
    ACTCCAACAG AGAAGCAAAA GAAAAAAAAG CTATTGTTTG AAAGAAGAAG 
    AAGTTAACTA TGGCAAAGGA TGTGGAAGCC GTTCCCGGAG AAGGATTTCA 
    GACAAGAGAC TATCAAGATC CGCCACCAGC TCCGTTTATT GATGGAGCGG 
    AGCTAAAGAA GTGGTCTTTC TACAGAGCAG TTATCGCAGA GTTCGTAGCC 
    ACTCTCCTCT TCTTATACAT CACCGTTTTG ACAGTCATCG GTTACAAGAT 
    TCAGTCCGAT ACTGATGCCG GTGGCGTAGA TTGCGGCGGA GTTGGAATCC 
    TCGGTATCGC TTGGGCCTTT GGTGGTATGA TCTTCATCCT CGTCTACTGC 
    ACCGCCGGTA TCTCTGGTGG TCACATTAAC CCAGCGGTGA CATTTGGGCT 
    ATTCTTGGCA CGTAAAGTGT CGTTACCTAG GGCCCTATTG TACATAATCG 
    CTCAGTGTTT GGGTGCGATT TGTGGAGTTG GTTTTGTCAA AGCCTTCCAA 
    AGCTCTTACT ACACCCGTTA CGGAGGTGGA GCCAACTCTC TAGCCGATGG 
    CTACAGCACA GGGACCGGTC TAGCCGCAGA GATCATTGGT ACTTTCGTTC 
    TTGTCTACAC CGTCTTCTCT GCCACTGACC CCAAACGTAG TGCCAGAGAC 
    TCCCACGTTC CGGTGTTGGC GCCACTTCCA ATCGGATTTG CCGTGTTCAT 
    GGTACATTTG GCTACCATTC CCATTACCGG AACCGGAATT AACCCGGCAA 
    GGAGTTTCGG AGCTGCCGTA ATCTACAACA AGAGCAAGCC ATGGGATGAC 
    CACTGGATAT TTTGGGTTGG ACCATTCATT GGAGCTGCGA TAGCTGCATT 
    CTACCACCAA TTCGTTCTGA GAGCTTCAGG TTCTAAGTCT CTTGGATCAT 
    TCAGAAGTGC TGCCAACGTC TAAACACATC AAACCAGATT TCTCAAGAAT 
    AAATGAAGTT GTGATCTCTC TGTACAAATC TAAATCAGTC ACTTGCAGTT 
    TTCATTTCCC TTTCTTATAT TTGTGTTTTC CACTTGCTCT TTTGTGAATT 
    TTCCTTCGGT GTAATTGTTT TTTCTTTCGC TTTTAAACGA GTGATTGGAA 
    TTATAATCAA TGAAATCAAA GAGGTTCCT

    Gene models with high sequence identity

    1. AT3G53420.1 [+] show detail - plasma membrane intrinsic protein 2A
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    superfamily Major intrinsic protein 6.5e-63
    HMMTigr Aquaporin 3.1e-88
    HMMPanther AQUAPORIN-RELATED 1e-190
    HMMPanther Major intrinsic protein 1e-190
    Gene3D Major intrinsic protein 2.3e-65
    FPrintScan Major intrinsic protein 6.5e-71
    FPrintScan Major intrinsic protein 6.5e-71
    FPrintScan Major intrinsic protein 6.5e-71
    FPrintScan Major intrinsic protein 6.5e-71
    FPrintScan Major intrinsic protein 6.5e-71
    FPrintScan Major intrinsic protein 6.5e-71
    HMMPfam Major intrinsic protein 3.3e-88
    PatternScan Major intrinsic protein, conserved site 0

    See the detailed result >

    AT3G53420.1

    Model type
    Protein coding
    Short Description
    plasma membrane intrinsic protein 2A
    Curator Summary
    a member of the plasma membrane intrinsic protein subfamily PIP2. localizes to the plasma membrane and exhibits water transport activity in Xenopus oocyte. expressed specifically in the vascular bundles and protein level increases slightly during leaf dev. When expressed in yeast cells can conduct hydrogen peroxide into those cells.
    Computational Description
    plasma membrane intrinsic protein 2A (PIP2A); FUNCTIONS IN: water channel activity; INVOLVED IN: response to water deprivation, response to salt stress, transport, hydrogen peroxide transmembrane transport, water transport; LOCATED IN: plasma membrane, chloroplast, vacuole, membrane; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Major intrinsic protein, conserved site (InterPro:IPR022357), Aquaporin (InterPro:IPR012269), Major intrinsic protein (InterPro:IPR000425); BEST Arabidopsis thaliana protein match is: plasma membrane intrinsic protein 2 (TAIR:AT2G37170.1); Has 10900 Blast hits to 10884 proteins in 2233 species: Archae - 81; Bacteria - 5210; Metazoa - 1477; Fungi - 457; Plants - 2523; Viruses - 2; Other Eukaryotes - 1150 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR