F10302

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F10302

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T2 seed (PO:0009010)
  • increased size (PATO:0000586)
    (Seed::Shape::large)
  • increased length (PATO:0000573)
    (Seed::Shape::long)

Invisible phenotypes

Original: F10302

Determination of introduced cDNA(s)

  • RAFL14-14-L14 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL14-14-L14 - Resource: pdz88429

    5 prime EST [+] show sequence

    Nucleotide sequence: RAFL14-14-L14 - 5 prime EST
    AACCGATGTA TTCTTCTCCT TGTCCTTCCT TACTCTCTAT ATTTTTCTCA 
    ATTCTCCGAT TTGATCCAAC GATTTCACAG ATCTCTGATC TAAATGGCAT
    CCAACGGTGA TAAAGGACTG ATCGTTAGCT TCGGCGAGAT GCTAATCGAT
    TTCGTCCCTA CCGAATCAGG CGTCTCTCTC GCCGAAGCTC CTGGATTCCT
    CAAAGCTCCA GGCGGTGCTC CCGCTAACGT CGCAATCGCT GTTTCTCGTC
    TCGGTGGACG ATCTGCTTTC GTCGGAAAAC TTGGAGACGA CGAGTTCGGT
    CATATGTTGG CTGGGATTTT GAGGAAGAAC GGTGTTGATG ATCAGGGGAT
    TAACTTCGAT ACTGGAGCTA GAACTGCTTT AGCTTTCGTT ACGTTACGTG
    CTGATGGAGA TCGTGAGTTT ATGTTTTACC GCAATCCTAG CGCCGATATG
    CTTCTTCGTC CTGATGAACT CAATCTCGAC CTCATCAGAT CCGCGAAAGT
    GTTTCACTAT GGATCAATAA GTTTGATAGT GGAGCCGNGT AGGTCAGCTC
    ATTTGAGGCC ATGGAAGTGG CGAAAGAAGC ANGAGCNCTT

    Gene models with high sequence identity

    1. AT2G31390.1 [+] show detail - pfkB-like carbohydrate kinase family protein
      E-value: 0; Score: 99.32

    3 prime EST [+] show sequence

    Nucleotide sequence: RAFL14-14-L14 - 3 prime EST
    CCATAATTTA TTTTTATTAC TTCAATCTTA AAACATCTTA CACAAACACC 
    ACATACTGTA TATATGAGCA ACAAATAAAT GATAAAAATA AATAAAAACT
    TGATTGAGTT ACAAAGGCAA TGCCAAGTAA CATATTCATG TATCAAAGAA
    TCAAGAATTT AAGTGCACTC GGCAGGAACT CCTCGAGAAC CGTGGACGTT
    GTTTCCTGTC GAAGTCGCAG ACTCGTCATC ACAGCTCAGG CGAGTAATTC
    TGAGTTTCTT CTATCTACGC GCAATATCTC TCAAAGGAGG CCTCTTCAAA
    GGTACCACAA TCATATCCTC TCGTTTCTAT CTGAAGAATC AGAGAGAAGA
    GATATATAGA GGATGCCTGT GATCTCCAAA GCTTCATCTA CCACCACCAA
    TTCATCGATT CCTTCGTGTT CTAGGATTGG AGG

    Gene models with high sequence identity

    1. AT2G43430.1 [+] show detail - glyoxalase 2-1
      E-value: 5e-133; Score: 100.00

    AT2G31390.1

    Model type
    Protein coding
    Short Description
    pfkB-like carbohydrate kinase family protein
    Computational Description
    pfkB-like carbohydrate kinase family protein; FUNCTIONS IN: kinase activity, ribokinase activity; INVOLVED IN: D-ribose metabolic process, acetate fermentation, sucrose biosynthetic process, sucrose catabolic process, using beta-fructofuranosidase; LOCATED IN: plasma membrane; EXPRESSED IN: 27 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: Carbohydrate/purine kinase (InterPro:IPR011611), Ribokinase (InterPro:IPR002139), Carbohydrate/puine kinase, PfkB, conserved site (InterPro:IPR002173); BEST Arabidopsis thaliana protein match is: pfkB-like carbohydrate kinase family protein (TAIR:AT1G06030.1); Has 18509 Blast hits to 18503 proteins in 2397 species: Archae - 386; Bacteria - 14053; Metazoa - 143; Fungi - 116; Plants - 466; Viruses - 0; Other Eukaryotes - 3345 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR

    AT2G43430.1

    Model type
    Protein coding
    Short Description
    glyoxalase 2-1
    Curator Summary
    Encodes a predicted mitochondrial glyoxalase GLX2-1. Studies using recombinant protein show that GLX2-1 contains a dinuclear metal binding site, but does not have glyoxalase 2 activity.
    Computational Description
    glyoxalase 2-1 (GLX2-1); CONTAINS InterPro DOMAIN/s: Beta-lactamase, class B, conserved site (InterPro:IPR001018), Beta-lactamase-like (InterPro:IPR001279), Hydroxyacylglutathione hydrolase (InterPro:IPR017782); BEST Arabidopsis thaliana protein match is: glyoxalase 2-4 (TAIR:AT1G06130.2); Has 15540 Blast hits to 15539 proteins in 2481 species: Archae - 339; Bacteria - 10160; Metazoa - 480; Fungi - 326; Plants - 230; Viruses - 0; Other Eukaryotes - 4005 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR