F10333

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F10333

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • increased size (PATO:0000586)
    (Seed::Shape::large)
  • increased length (PATO:0000573)
    (Seed::Shape::long)
  • decreased width (PATO:0000599)
    (Seed::Shape::narrow)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F10333

Determination of introduced cDNA(s)

  • RAFL05-21-H04 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-21-H04 - Resource: pda02818

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-21-H04 - full reading 
    TGGTACCAAG AAGATTGCGG AGAGTCGTGA AAAGTTTTGA GCTTTGATCA 
    CTCACTCACT AGTAATGGAG GCTCTTCAAT TTTCCAGCGT GAATAGAGTT 
    CCTTGTACTC TGAGCTGTAC CGGAAATAGG AGAATTAAGG CTGCCTTCAG 
    CTCTGCGTTC ACCGGTGGCA CCATCAATTC AGCTTCTCTG TCCTCCTCTA 
    GGAATCTCTC CACGCGAGAG ATATGGAGTT GGGTGAAATC GAAGACTGTT 
    GTTGGCCATG GAAGATACAG AAGAAGCCAA GTGAGAGCCG AGATGTTTGG 
    TCAGTTGACT GGTGGCCTCG AGGCTGCTTG GAGCAAACTC AAAGGCGAAG 
    AGGTCTTGAC TAAGGACAAT ATTGCTGAGC CAATGCGGGA TATCAGAAGA 
    GCTCTCCTGG AAGCAGATGT GAGTCTCCCA GTTGTTAGAA GGTTTGTTCA 
    GTCTGTTAGT GACCAAGCCG TTGGAATGGG TGTCATTCGT GGAGTCAAAC 
    CAGATCAGCA ATTGGTCAAG ATTGTACATG ATGAGCTAGT GAAATTGATG 
    GGTGGAGAAG TATCTGAGTT ACAGTTTGCT AAATCAGGTC CTACAGTAAT 
    ATTGTTGGCT GGGCTCCAAG GAGTTGGAAA GACAACGGTT TGTGCTAAAC 
    TAGCTTGTTA CCTAAAGAAG CAGGGAAAAT CTTGCATGCT TATTGCTGGA 
    GATGTATACC GACCTGCTGC TATCGATCAA CTTGTCATTT TAGGTGAACA 
    GGTTGGTGTG CCGGTTTATA CAGCAGGGAC TGATGTAAAA CCTGCAGATA 
    TAGCTAAGCA AGGTCTAAAA GAAGCTAAAA AGAACAATGT TGATGTAGTT 
    ATTATGGATA CTGCAGGGAG GCTTCAGATA GATAAAGGCA TGATGGACGA 
    ATTAAAAGAC GTGAAGAAAT TTCTGAATCC AACAGAAGTG TTACTAGTTG 
    TTGATGCCAT GACTGGACAA GAAGCTGCAG CACTGGTGAC GACATTCAAT 
    GTAGAGATAG GAATTACAGG AGCCATTTTG ACAAAGCTAG ATGGTGATTC 
    AAGAGGTGGT GCTGCTTTGA GTGTCAAGGA GGTATCAGGA AAACCGATTA 
    AACTGGTAGG ACGTGGAGAG CGAATGGAGG ATCTTGAACC CTTTTATCCA 
    GATCGAATGG CTGGACGAAT TCTAGGAATG GGAGATGTGC TTTCATTTGT 
    AGAGAAGGCA ACAGAAGTTA TGCGTCAAGA AGATGCAGAA GATCTGCAGA 
    AGAAGATAAT GAGTGCAAAG TTCGACTTCA ACGATTTTCT AAAGCAGACT 
    CGTGCTGTAG CGAAAATGGG TTCGATGACA CGAGTTCTTG GAATGATTCC 
    CGGAATGGGG AAAGTGAGTC CAGCACAAAT CCGAGAAGCG GAAAAGAATC 
    TTTTAGTCAT GGAAGCAATG ATTGAAGTGA TGACACCCGA GGAAAGAGAG 
    AGACCAGAGT TACTAGCAGA ATCTCCAGAG AGAAGAAAAA GAATCGCTAA 
    AGATTCAGGA AAGACAGAAC AACAGGTGAG CGCACTTGTA GCACAAATCT 
    TCCAAATGAG AGTGAAAATG AAGAACTTGA TGGGAGTGAT GGAAGGAGGA 
    TCAATTCCAG CATTAAGCGG TCTCGAGGAC GCATTGAAAG CAGAACAAAA 
    GGCTCCACCT GGAACTGCAA GGAGGAAGAG AAAGGCAGAC TCAAGGAAGA 
    AATTTGTAGA GTCAGCATCA AGCAAGCCTG GTCCTCGTGG CTTCGGCTCT 
    GGTAACTAAA ACTATTTCAG CCCAATTTGG TTAATTTCTA AACGGATTAT 
    TTTTTTGTAT AAGTTAATGA ATGAGACAGA AACCTTACAA GTAAAAAAAA 
    AAAAAAAA

    Gene models with high sequence identity

    1. AT5G03940.1 [+] show detail - chloroplast signal recognition particle 54 kDa subunit
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPanther Signal recognition particle, SRP 2e-234
    HMMPanther GTPASE CONTAINING FAMILY OF SIGNAL RECOGNITIO 2e-234
    Gene3D Signal recognition particle, SRP54 subunit, helical bundle 1.9e-26
    Gene3D no description 6.6e-70
    Gene3D Signal recognition particle, SRP54 subunit, M-domain 3.1e-28
    PatternScan Signal recognition particle, SRP54 subunit, GTPase 0
    HMMPfam Signal recognition particle, SRP54 subunit, GTPase 7.9e-77
    HMMPfam Signal recognition particle, SRP54 subunit, M-domain 1.8e-25
    HMMPfam Signal recognition particle, SRP54 subunit, helical bundle 1e-18
    HMMSmart ATPase, AAA+ type, core 0.000000000026
    HMMTigr Signal recognition particle, SRP 3.4e-224
    superfamily P-loop containing nucleoside triphosphate hyd 2.4e-72
    superfamily Signal recognition particle, SRP54 subunit, M-domain 4.8e-40
    superfamily Signal recognition particle, SRP54 subunit, helical bundle 1.8e-22

    See the detailed result >

    AT5G03940.1

    Model type
    Protein coding
    Short Description
    chloroplast signal recognition particle 54 kDa subunit
    Curator Summary
    mutant has Yellow first leaves; Chloroplast Signal Recognition Particle Subunit
    Computational Description
    chloroplast signal recognition particle 54 kDa subunit (CPSRP54); FUNCTIONS IN: 7S RNA binding, protein binding, mRNA binding, GTP binding, signal sequence binding; INVOLVED IN: protein import into chloroplast thylakoid membrane, SRP-dependent cotranslational protein targeting to membrane, signal sequence recognition; LOCATED IN: chloroplast stroma, chloroplast, signal recognition particle, chloroplast targeting, signal recognition particle, endoplasmic reticulum targeting; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: ATPase, AAA+ type, core (InterPro:IPR003593), Signal recognition particle, SRP (InterPro:IPR004780), Signal recognition particle, SRP54 subunit, helical bundle (InterPro:IPR013822), Signal recognition particle, SRP54 subunit, M-domain (InterPro:IPR004125), Signal recognition particle, SRP54 subunit, GTPase (InterPro:IPR000897); BEST Arabidopsis thaliana protein match is: Signal recognition particle, SRP54 subunit protein (TAIR:AT1G48900.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR