F10436

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F10436

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
whole plant (PO:0000003)
  • increased height (PATO:0000570)
    (Adult plant::Plant height::tall)
cauline leaf (PO:0000013)
  • increased size (PATO:0000586)
    (Cauline leaf::Shape::large)
  • epinastic (PATO:0000945)
    (Cauline leaf::Shape::epinastic)
  • dark green (PATO:0001249)
    (Cauline leaf::Color::dark)
rosette leaf (PO:0000014)
  • increased size (PATO:0000586)
    (Rosette leaf::Shape::large)
stem internode (PO:0020142)
  • decreased length (PATO:0000574)
    (Stem::Shape::short internode)
T2  
cauline leaf (PO:0000013)
  • dark green (PATO:0001249)
    (Cauline leaf::Color::dark)
rosette leaf (PO:0000014)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)
  • increased size (PATO:0000586)
    (Rosette leaf::Shape::large)
  • present in greater numbers in organism (PATO:0000470)
    (Rosette leaf::Number::many)
stem (PO:0009047)
  • increased thickness (PATO:0000591)
    (Stem::Shape::thick)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F10436

Determination of introduced cDNA(s)

  • RAFL09-14-H22 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL09-14-H22 - Resource: pda01774

    full reading [+] show sequence

    Nucleotide sequence: RAFL09-14-H22 - full reading 
    AAAAACATAA AGAATCAGCA AAGCAAAATT CGAAGAGACA CAAAAAAACC 
    TTCGTTTGTG TCTCAGAATT TACAAAGTTT TGAGTCAGTA GAGAAATGGC 
    GGGAATCGTG ACAGAGCCGT GGTCAGTAGC TGAGAACGGA AACCCAAGCA 
    TAACGGCGAA AGGATCGAGC AGAGAACTAA GACTTGGAAG AACCGCTCAC 
    AACATGTCTT CTTCTTCTTT GAGGAAAAAA TCAGACCTCC GAGTGATTCA 
    GAAGGTTCCA TACAAAGGTC TTAAAGATTT TCTCTCTAAT CTCCAAGAAG 
    TCATTCTCGG AACAAAGCTT GCCATTCTTT TTCCGGCCAT TCCTGCCGCC 
    ATTATTTGCA CCTATTGTGG CGTCAGTCAG CCTTGGATAT TTGGACTTAG 
    CTTGCTAGGA CTGACACCTT TGGCTGAGCG AGTCAGCTTT TTGACAGAGC 
    AACTAGCTTT CTACACCGGT CCTACATTGG GTGGTCTATT GAACGCAACG 
    TGTGGAAACG CGACTGAATT GATAATCGCG ATTCTTGCTT TGACCAATAA 
    CAAAGTCGCA GTGGTGAAAT ATTCGCTGCT AGGTTCGATT TTGTCGAACC 
    TTTTATTGGT TCTAGGGACT TCACTCTTCT GTGGAGGAAT CGCTAATATC 
    CGAAGGGAAC AGCGGTTCGA CCGGAAACAA GCCGATGTGA ACTTCTTCTT 
    ACTTCTACTG GGTTTCTTGT GTCACTTGCT GCCATTGTTG GTGGGATACT 
    TGAAAAACGG AGAGGCTTCG GCTGCTGTTT TGTCCGACAT GCAACTGAGT 
    ATATCGCGAG GCTTCAGTAT TGTTATGTTG ATCAGCTACA TTGCATATCT 
    TGTTTTCCAA CTGTGGACTC ACCGCCAATT GTTCGATGCA CAAGAACAGG 
    AAGATGAGTA TGATGACGAT GTGGAGCAAG AAACCGCGGT GATTAGTTTT 
    TGGAGCGGTT TTGCATGGTT GGTTGGGATG ACACTCGTTA TCGCATTGCT 
    ATCGGAGTAT GTTGTAGCCA CGATTGAGGA AGCATCGGAT AAATGGAACT 
    TATCAGTGAG TTTCATAAGC ATCATATTGC TTCCTATTGT TGGAAATGCA 
    GCTGAACATG CTGGAGCCGT TATTTTTGCC TTTAAGAACA AGCTTGACAT 
    ATCTTTGGGA GTTGCGTTAG GCTCTGCGAC TCAGATTGGC TTATTCGTTG 
    TCCCCTTGAC CATCATCGTG GCGTGGATTT TGGGAATTAA TATGGATCTC 
    AATTTTGGTC CCCTCGAAAC TGGCTGTCTT GCTGTTTCCA TAATCATCAC 
    AGCGTTCACA TTACAGGATG GGAGTTCTCA CTACATGAAG GGACTGGTCC 
    TCTTGCTTTG CTATTTCATT ATTGCCATCT GTTTCTTCGT CGACAAACTT 
    CCCCAGAAAC AAAATGCCAT TCACTTGGGA CATCAAGCGA TGAACAATGT 
    CGTCACTGCA ACAGGAGGAG GAGTTTTCTC ATCTTAAACG AAGACAAGTA 
    CCAATGTGTT GAAACAAAAT TTCCAAAGCG AGAGACAATG GAGAAAGGAA 
    AACAAAAACC AATTTTCTTG ATTCATTTAT AAATATTGAA AGATTTGTCA 
    GAGAGATTCC TGAGATTCAG TGAGTATTAT TTCATTTTTC AAAAATGTTG 
    GGTGTCTTGA TGAAGATGAG TTTTGAGAAC TTTATAAATT CTTCTTGTGG 
    GTGGGTTTTG CAATGAACTA TCTATGAATG TCTTGTGTCA AATGTTCTAT 
    CTCTTTTTAT TAATGGTTTT ATCATTTTTT CTGAGG

    Gene models with high sequence identity

    1. AT2G38170.1 [+] show detail - cation exchanger 1
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMTigr Calcium/proton exchanger 3.7e-237
    HMMTigr Calcium/proton exchanger superfamily 2.5e-88
    HMMPfam Sodium/calcium exchanger membrane region 6.1e-22
    HMMPfam Sodium/calcium exchanger membrane region 2.1e-18

    See the detailed result >

    AT2G38170.1

    Model type
    Protein coding
    Short Description
    cation exchanger 1
    Curator Summary
    Encodes a high affinity vacuolar calcium antiporter. The residue His 338 is critical to Ca2+ transport activity. Disruption of CAX1 reduces manganese and zinc of shoot tissue and results in a decrease in the activity of vacuolar V-type proton ATPase.
    Computational Description
    cation exchanger 1 (CAX1); FUNCTIONS IN: calcium:cation antiporter activity, calcium ion transmembrane transporter activity, calcium:hydrogen antiporter activity; INVOLVED IN: cold acclimation, response to salt stress, cellular zinc ion homeostasis, cellular manganese ion homeostasis, calcium ion transport; LOCATED IN: plant-type vacuole membrane, vacuolar membrane, vacuole, membrane; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Sodium/calcium exchanger membrane region (InterPro:IPR004837), Calcium/proton exchanger superfamily (InterPro:IPR004798), Calcium/proton exchanger (InterPro:IPR004713); BEST Arabidopsis thaliana protein match is: cation exchanger 3 (TAIR:AT3G51860.1); Has 3042 Blast hits to 2882 proteins in 950 species: Archae - 18; Bacteria - 1703; Metazoa - 62; Fungi - 722; Plants - 217; Viruses - 0; Other Eukaryotes - 320 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR