F10521

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F10521

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • increased size (PATO:0000586)
    (Seed::Shape::large)
  • increased length (PATO:0000573)
    (Seed::Shape::long)

Invisible phenotypes

Original: F10521

Determination of introduced cDNA(s)

  • RAFL02-06-F22 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL02-06-F22 - Resource: pda00045

    full reading [+] show sequence

    Nucleotide sequence: RAFL02-06-F22 - full reading 
    ACCAGAAGAA GAGCCACACA CTCACAAATT AAAAAGAGAG AGAGAGAGAG 
    AGAGACAGAG AGAGAGAGAG ATTCTGCGGA GGAGCTTCTT CTTCGTAGGG 
    TGTTCATCGT TATTAACGTT ATCGCCCCTA CGTCAGCTCC ATCTCCAGAA 
    ACATGGGTGC AGGTGGAAGA ATGCCGGTTC CTACTTCTTC CAAGAAATCG 
    GAAACCGACA CCACAAAGCG TGTGCCGTGC GAGAAACCGC CTTTCTCGGT 
    GGGAGATCTG AAGAAAGCAA TCCCGCCGCA TTGTTTCAAA CGCTCAATCC 
    CTCGCTCTTT CTCCTACCTT ATCAGTGACA TCATTATAGC CTCATGCTTC 
    TACTACGTCG CCACCAATTA CTTCTCTCTC CTCCCTCAGC CTCTCTCTTA 
    CTTGGCTTGG CCACTCTATT GGGCCTGTCA AGGCTGTGTC CTAACTGGTA 
    TCTGGGTCAT AGCCCACGAA TGCGGTCACC ACGCATTCAG CGACTACCAA 
    TGGCTGGATG ACACAGTTGG TCTTATCTTC CATTCCTTCC TCCTCGTCCC 
    TTACTTCTCC TGGAAGTATA GTCATCGCCG TCACCATTCC AACACTGGAT 
    CCCTCGAAAG AGATGAAGTA TTTGTCCCAA AGCAGAAATC AGCAATCAAG 
    TGGTACGGGA AATACCTCAA CAACCCTCTT GGACGCATCA TGATGTTAAC 
    CGTCCAGTTT GTCCTCGGGT GGCCCTTGTA CTTAGCCTTT AACGTCTCTG 
    GCAGACCGTA TGACGGGTTC GCTTGCCATT TCTTCCCCAA CGCTCCCATC 
    TACAATGACC GAGAACGCCT CCAGATATAC CTCTCTGATG CGGGTATTCT 
    AGCCGTCTGT TTTGGTCTTT ACCGTTACGC TGCTGCACAA GGGATGGCCT 
    CGATGATCTG CCTCTACGGA GTACCGCTTC TGATAGTGAA TGCGTTCCTC 
    GTCTTGATCA CTTACTTGCA GCACACTCAT CCCTCGTTGC CTCACTACGA 
    TTCATCAGAG TGGGACTGGC TCAGGGGAGC TTTGGCTACC GTAGACAGAG 
    ACTACGGAAT CTTGAACAAG GTGTTCCACA ACATTACAGA CACACACGTG 
    GCTCATCACC TGTTCTCGAC AATGCCGCAT TATAACGCAA TGGAAGCTAC 
    AAAGGCGATA AAGCCAATTC TGGGAGACTA TTACCAGTTC GATGGAACAC 
    CGTGGTATGT AGCGATGTAT AGGGAGGCAA AGGAGTGTAT CTATGTAGAA 
    CCGGACAGGG AAGGTGACAA GAAAGGTGTG TACTGGTACA ACAATAAGTT 
    ATGAGGATGA TGGTGAAGAA ATTGTCGACC TTTCTCTTGT CTGTTTGTCT 
    TTTGTTAAAG AAGCTATGCT TCGTTTTAAT AATCTTATTG TCCATTTTGT 
    TGTGTTATGA CATTTTGGCT GCTCATTATG TTATGTGGGA AGTTAGTGTT 
    C

    Gene models with high sequence identity

    1. AT3G12120.1 [+] show detail - fatty acid desaturase 2
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Fatty acid desaturase, type 1 2.6e-33
    HMMPfam Protein of unknown function DUF3474 4.6e-07

    See the detailed result >

    AT3G12120.1

    Model type
    Protein coding
    Short Description
    fatty acid desaturase 2
    Curator Summary
    Major enzyme responsible for the synthesis of 18:2 fatty acids in the endoplasmic reticulum. Contains His-rich motifs, which contribute to the interaction with the electron donor cytochrome b5. Mutations in this gene suppress the low temperature-induced phenotype of Arabidopsis tocopherol-deficient mutant vte2.
    Computational Description
    fatty acid desaturase 2 (FAD2); FUNCTIONS IN: omega-6 fatty acid desaturase activity, delta12-fatty acid dehydrogenase activity; INVOLVED IN: oxidation reduction, lipid metabolic process; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF3474 (InterPro:IPR021863), Fatty acid desaturase, type 1 (InterPro:IPR005804); BEST Arabidopsis thaliana protein match is: fatty acid desaturase 3 (TAIR:AT2G29980.1); Has 2431 Blast hits to 2431 proteins in 654 species: Archae - 0; Bacteria - 821; Metazoa - 31; Fungi - 266; Plants - 965; Viruses - 0; Other Eukaryotes - 348 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR