F10521

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F10521

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T2 seed (PO:0009010)
  • increased size (PATO:0000586)
    (Seed::Shape::large)
  • increased length (PATO:0000573)
    (Seed::Shape::long)

Invisible phenotypes

Original: F10521

Determination of introduced cDNA(s)

  • RAFL02-06-F22 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL02-06-F22 - Resource: pda00045

    full reading [+] show sequence

    Nucleotide sequence: RAFL02-06-F22 - full reading
    ACCAGAAGAA GAGCCACACA CTCACAAATT AAAAAGAGAG AGAGAGAGAG 
    AGAGACAGAG AGAGAGAGAG ATTCTGCGGA GGAGCTTCTT CTTCGTAGGG
    TGTTCATCGT TATTAACGTT ATCGCCCCTA CGTCAGCTCC ATCTCCAGAA
    ACATGGGTGC AGGTGGAAGA ATGCCGGTTC CTACTTCTTC CAAGAAATCG
    GAAACCGACA CCACAAAGCG TGTGCCGTGC GAGAAACCGC CTTTCTCGGT
    GGGAGATCTG AAGAAAGCAA TCCCGCCGCA TTGTTTCAAA CGCTCAATCC
    CTCGCTCTTT CTCCTACCTT ATCAGTGACA TCATTATAGC CTCATGCTTC
    TACTACGTCG CCACCAATTA CTTCTCTCTC CTCCCTCAGC CTCTCTCTTA
    CTTGGCTTGG CCACTCTATT GGGCCTGTCA AGGCTGTGTC CTAACTGGTA
    TCTGGGTCAT AGCCCACGAA TGCGGTCACC ACGCATTCAG CGACTACCAA
    TGGCTGGATG ACACAGTTGG TCTTATCTTC CATTCCTTCC TCCTCGTCCC
    TTACTTCTCC TGGAAGTATA GTCATCGCCG TCACCATTCC AACACTGGAT
    CCCTCGAAAG AGATGAAGTA TTTGTCCCAA AGCAGAAATC AGCAATCAAG
    TGGTACGGGA AATACCTCAA CAACCCTCTT GGACGCATCA TGATGTTAAC
    CGTCCAGTTT GTCCTCGGGT GGCCCTTGTA CTTAGCCTTT AACGTCTCTG
    GCAGACCGTA TGACGGGTTC GCTTGCCATT TCTTCCCCAA CGCTCCCATC
    TACAATGACC GAGAACGCCT CCAGATATAC CTCTCTGATG CGGGTATTCT
    AGCCGTCTGT TTTGGTCTTT ACCGTTACGC TGCTGCACAA GGGATGGCCT
    CGATGATCTG CCTCTACGGA GTACCGCTTC TGATAGTGAA TGCGTTCCTC
    GTCTTGATCA CTTACTTGCA GCACACTCAT CCCTCGTTGC CTCACTACGA
    TTCATCAGAG TGGGACTGGC TCAGGGGAGC TTTGGCTACC GTAGACAGAG
    ACTACGGAAT CTTGAACAAG GTGTTCCACA ACATTACAGA CACACACGTG
    GCTCATCACC TGTTCTCGAC AATGCCGCAT TATAACGCAA TGGAAGCTAC
    AAAGGCGATA AAGCCAATTC TGGGAGACTA TTACCAGTTC GATGGAACAC
    CGTGGTATGT AGCGATGTAT AGGGAGGCAA AGGAGTGTAT CTATGTAGAA
    CCGGACAGGG AAGGTGACAA GAAAGGTGTG TACTGGTACA ACAATAAGTT
    ATGAGGATGA TGGTGAAGAA ATTGTCGACC TTTCTCTTGT CTGTTTGTCT
    TTTGTTAAAG AAGCTATGCT TCGTTTTAAT AATCTTATTG TCCATTTTGT
    TGTGTTATGA CATTTTGGCT GCTCATTATG TTATGTGGGA AGTTAGTGTT
    C

    Gene models with high sequence identity

    1. AT3G12120.1 [+] show detail - fatty acid desaturase 2
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Fatty acid desaturase, type 1 2.6e-33
    HMMPfam Protein of unknown function DUF3474 4.6e-07

    See the detailed result >

    AT3G12120.1

    Model type
    Protein coding
    Short Description
    fatty acid desaturase 2
    Curator Summary
    Major enzyme responsible for the synthesis of 18:2 fatty acids in the endoplasmic reticulum. Contains His-rich motifs, which contribute to the interaction with the electron donor cytochrome b5. Mutations in this gene suppress the low temperature-induced phenotype of Arabidopsis tocopherol-deficient mutant vte2.
    Computational Description
    fatty acid desaturase 2 (FAD2); FUNCTIONS IN: omega-6 fatty acid desaturase activity, delta12-fatty acid dehydrogenase activity; INVOLVED IN: oxidation reduction, lipid metabolic process; LOCATED IN: endoplasmic reticulum; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF3474 (InterPro:IPR021863), Fatty acid desaturase, type 1 (InterPro:IPR005804); BEST Arabidopsis thaliana protein match is: fatty acid desaturase 3 (TAIR:AT2G29980.1); Has 2431 Blast hits to 2431 proteins in 654 species: Archae - 0; Bacteria - 821; Metazoa - 31; Fungi - 266; Plants - 965; Viruses - 0; Other Eukaryotes - 348 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR