F10724

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F10724

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • increased size (PATO:0000586)
    (Seed::Shape::large)
  • increased length (PATO:0000573)
    (Seed::Shape::long)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F10724

Determination of introduced cDNA(s)

  • RAFL06-69-D03 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL06-69-D03 - Resource: pda09445

    full reading [+] show sequence

    Nucleotide sequence: RAFL06-69-D03 - full reading 
    AGTTTCAGCG AAACGATGGA GCTTCTCTGC TCACCAACGT CTCTTTCCTC 
    CTCCTTCGCT CTCTCTTCTG CTCTTTTAGT CCCTCGTTCG TTCTCCATGC 
    CAGGGACAAG GAGATTCATG GTTCTGTGTA GTTCCCAGAA GGAAAGTCAG 
    ATGACAGTGT CAGTAACTGG AGCAACTGGA TTCATAGGGA GACGATTGGT 
    TCAGAGACTT CGTGCTGATA ACCACGCTAT CCGGGTCTTA ACACGCTCTA 
    AATCCAAAGC TGAGCAAATA TTTCCTGCCA AGGACTTCCC AGGTATTGTG 
    ATTGCAGAAG AATCGGAGTG GAAGAACTGT GTACAAGGCT CTACTGCTGT 
    TGTGAACTTG GCAGGACTGC CTATTAGTAC AAGGTGGTCT CCCGAGATCA 
    AGAAAGAGAT TAAGGGCAGC CGGATCCGTG TCACCTCCAA AGTTGTTGAC 
    TTAATCAATA ATTCACCAGC AGAAGCTAGG CCTACCGTTC TAGTTAGCGC 
    CACGGCTGTT GGTTACTATG GTACTAGTGA GACAGGAGTA TTCGACGAAA 
    ATAGTCCATC AGGGAAGGAC TATCTTGCAG AGGTTTGCAG AGAATGGGAA 
    GGAACTGCAT TGAAAGCAAA TAAGGATGTT AGAGTGGCAC TTATCCGCAT 
    TGGTGTTGTT CTTGGCAAAG ACGGTGGAGC TTTGGCCATG ATGATACCCT 
    TTTTCCAAAT GTTTGCTGGA GGACCTCTGG GTTCCGGACA ACAATGGTTC 
    TCTTGGATTC ACGTGGATGA CTTGGTGAAC TTAATTTACG AGGCCCTCAC 
    TAATCCATCT TATAAAGGAG TTATTAATGG GACTGCGCCG AATCCGGTTA 
    GGCTTGGGGA AATGTGCCAG CAGCTAGGCA GTGTTCTTAG CCGACCATCG 
    TGGCTACCAG TTCCTGACTT TGCACTCAAA GCTTTGCTCG GAGAAGGTGC 
    CACTGTAGTT CTGGAGGGAC AGAAGGTATT ACCTGTAAGA GCCAAAGAGC 
    TTGGTTTTGA ATTCAAGTAT AAATACGTGA AGGATGCACT CAGAGCCATT 
    ATGCAATAAG GTGACACACA TAAGCATCTT TGTAAAATTG GTTTTGATCA 
    CAGAAAATCC CCAGAAAAAC AAGATATTCT TTTCTTTTCG TCTCTATATC 
    TTTCTTTTTG AGTAAAACTC GAGAAATGTA TTTATGGTAA AAAGTGTTTT 
    CGGTGTTATC TCAGAAACCA GAGATCACAG ATGTGTTGAG GATATTATAA 
    AAAAAAAAAA AATAAAAAAA AAAAAAAA

    Gene models with high sequence identity

    1. AT2G21280.1 [+] show detail - NAD(P)-binding Rossmann-fold superfamily protein
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMTigr Sugar nucleotide epimerase YfcH, putative 6.8e-173
    HMMPanther Sugar nucleotide epimerase YfcH, putative 3.5e-82
    Gene3D NAD(P)-binding domain 1.3e-15
    HMMPfam NAD-dependent epimerase/dehydratase 1.6e-17
    HMMPfam Domain of unknown function DUF1731, C-terminal 2.4e-17
    superfamily NAD(P)-binding domain 1.5e-40

    See the detailed result >

    AT2G21280.1

    Model type
    Protein coding
    Short Description
    NAD(P)-binding Rossmann-fold superfamily protein
    Curator Summary
    A nuclear-encoded, plastid-targeted protein (AtSulA) whose overexpression causes severe yet stochastic plastid (shown in chloroplasts and leucoplasts) division defects. The protein does not appear to interact with either AtFtsZ proteins when studied in a yeast two-hybrid system.
    Computational Description
    SULA; FUNCTIONS IN: coenzyme binding, binding, catalytic activity; INVOLVED IN: chloroplast fission; LOCATED IN: chloroplast, plastid, chloroplast envelope; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Sugar nucleotide epimerase YfcH, putative (InterPro:IPR010099), NAD-dependent epimerase/dehydratase (InterPro:IPR001509), Domain of unknown function DUF1731, C-terminal (InterPro:IPR013549), NAD(P)-binding domain (InterPro:IPR016040); BEST Arabidopsis thaliana protein match is: NAD(P)-binding Rossmann-fold superfamily protein (TAIR:AT4G33360.2); Has 4854 Blast hits to 4853 proteins in 1452 species: Archae - 38; Bacteria - 3329; Metazoa - 111; Fungi - 30; Plants - 185; Viruses - 0; Other Eukaryotes - 1161 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR