F11236

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F11236

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
cauline leaf (PO:0000013)
  • decreased size (PATO:0000587)
    (Cauline leaf::Shape::small)
rosette leaf (PO:0000014)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
  • decreased width (PATO:0000599)
    (Rosette leaf::Shape::narrow)
  • present in fewer numbers in organism (PATO:0001997)
    (Rosette leaf::Number::few)
petal (PO:0009032)
  • abnormal (PATO:0000460)
    (Flower::Shape::petal)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F11236

Determination of introduced cDNA(s)

  • RAFL05-14-N11 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-14-N11 - Resource: pda17086

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-14-N11 - full reading 
    GATCATCTCA TTAACAAAAA TAAAACACAC AATCTCAAGA TTTTCTACTT 
    CTTATTACAA AGATTCAATC TTCTTGTTTC TTCTTGCAAC CATGAGTCTT 
    CTTGCAGATC TTGTTAACCT TGACATCTCA GACAACAGTG AAAAGATCAT 
    CGCTGAATAC ATATGGGTTG GTGGTTCTGG TATGGACATG AGAAGCAAAG 
    CCAGGACTCT CCCTGGACCT GTGACCGATC CATCAAAACT TCCAAAGTGG 
    AACTATGATG GTTCAAGCAC TGGTCAAGCT CCTGGTCAAG ACAGTGAAGT 
    GATCTTATAC CCTCAAGCAA TTTTCAAAGA TCCATTCCGT AGAGGCAACA 
    ACATCCTTGT TATGTGTGAT GCTTACACTC CAGCGGGAGA GCCAATCCCT 
    ACTAACAAGC GACATGCTGC GGCTGAGATC TTTGCTAACC CTGATGTTAT 
    TGCTGAAGTG CCATGGTATG GAATCGAACA AGAATACACT TTGTTGCAGA 
    AGGATGTGAA CTGGCCTCTT GGATGGCCCA TTGGTGGCTT CCCTGGCCCT 
    CAGGGACCAT ACTACTGCAG TATTGGAGCT GACAAATCTT TTGGAAGAGA 
    CATTGTTGAT GCTCACTACA AAGCCTCTTT GTATGCTGGA ATCAACATCA 
    GTGGGATCAA TGGAGAAGTC ATGCCGGGAC AATGGGAGTT CCAAGTCGGC 
    CCATCGGTCG GTATCTCAGC TGCTGATGAA ATATGGATCC CTCGTTACAT 
    TTTGGAGAGG ATCACAGAGA TTGCTGGTGT GGTTGTATCT TTTGACCCAA 
    AACCTATTCC TGGTGACTGG AATGGAGCTG GTGCTCACAC CAATTACAGT 
    ACTAAATCAA TGAGGGAAGA AGGAGGATAC GAGATAATCA AGAAGGCGAT 
    CGAGAAGCTT GGCTTGAGAC ACAAGGAACA CATTTCCGCT TACGGTGAAG 
    GAAACGAGCG TCGTCTCACG GGACACCATG AAACTGCTGA CATCAACACT 
    TTCCTTTGGG GTGTTGCGAA CCGTGGTGCA TCGATCCGAG TAGGACGTGA 
    CACCGAGAAA GAAGGGAAGG GATACTTTGA GGATAGGAGG CCAGCTTCAA 
    ACATGGACCC TTACGTTGTT ACTTCCATGA TTGCAGAGAC TACACTCCTC 
    TGGAACCCTT GAAAGGATGA TCCGTAACTC TTGAAGTTGC TTCTGATTGG 
    GTTTTTTGGA AGTTCCAAGC TTGTCTTTTC TCTACAGTGT GTATTAAGCA 
    ATTGTACCGG TTGACACTGC CGGAGTTTGT GATTTGGGGC CTTTCTTTCT 
    TTTTCTTCTT TTTATAATCT TTTGGGTTCT GTGGTTAGAG CAAATTCGGT 
    TTGCTCTGTT TGTTTGACCT TTATTGAAAC CTTTGGTATT GGTACTAATA 
    ATACAATCTG AAAAGGCCTC TTCATGTTCC AAAAAAAAAA

    Gene models with high sequence identity

    1. AT1G66200.1 [+] show detail - glutamine synthase clone F11
      E-value: 0; Score: 99.93

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Glutamine synthetase, catalytic domain 9.1e-61
    HMMPfam Glutamine synthetase, beta-Grasp 1.1e-18
    Gene3D Glutamine synthetase/guanido kinase, catalytic domain 1.2e-55
    PatternScan Glutamine synthetase, beta-Grasp 0
    PatternScan Glutamine synthetase, catalytic domain 0
    superfamily Glutamine synthetase/guanido kinase 7.4e-67
    superfamily Glutamine synthetase, beta-Grasp 8.5e-18
    HMMPanther GLUTAMINE SYNTHETASE (GLUTAMATE--AMMONIA LIGA 4.2e-259
    HMMPanther GLUTAMINE SYNTHETASE 4.2e-259

    See the detailed result >

    AT1G66200.1

    Model type
    Protein coding
    Short Description
    glutamine synthase clone F11
    Curator Summary
    encodes a cytosolic glutamate synthetase, this enzyme has low affinity with substrate ammonium
    Computational Description
    glutamine synthase clone F11 (GSR2); FUNCTIONS IN: glutamate-ammonia ligase activity, copper ion binding; INVOLVED IN: nitrate assimilation, response to fructose stimulus, response to salt stress, response to sucrose stimulus, response to glucose stimulus; LOCATED IN: cytosol, cytosolic ribosome, apoplast, vacuole, chloroplast envelope; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Glutamine synthetase, catalytic domain (InterPro:IPR008146), Glutamine synthetase, beta-Grasp (InterPro:IPR008147), Glutamine synthetase/guanido kinase, catalytic domain (InterPro:IPR014746); BEST Arabidopsis thaliana protein match is: glutamine synthase clone R1 (TAIR:AT5G37600.1); Has 11094 Blast hits to 11090 proteins in 3405 species: Archae - 162; Bacteria - 5924; Metazoa - 440; Fungi - 257; Plants - 1757; Viruses - 3; Other Eukaryotes - 2551 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR