F11912

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F11912

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
cauline leaf (PO:0000013)
  • epinastic (PATO:0000945)
    (Cauline leaf::Shape::epinastic)
  • abnormal (PATO:0000460)
    (Cauline leaf::Others)
rosette leaf (PO:0000014)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
sporophyte vegetative stage (PO:0007134)
  • decreased speed (PATO:0000304)
    (Adult plant::Growth::slow)
stem (PO:0009047)
  • abnormal (PATO:0000460)
    (Stem::Others)
T2  
cauline leaf (PO:0000013)
  • increased size (PATO:0000586)
    (Cauline leaf::Shape::large)
  • abnormal (PATO:0000460)
    (Cauline leaf::Others)
rosette leaf (PO:0000014)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
  • present in greater numbers in organism (PATO:0000470)
    (Rosette leaf::Number::many)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F11912

Determination of introduced cDNA(s)

  • RAFL14-14-L14 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL14-14-L14 - Resource: pdz88429

    5 prime EST [+] show sequence

    Nucleotide sequence: RAFL14-14-L14 - 5 prime EST 
    AACCGATGTA TTCTTCTCCT TGTCCTTCCT TACTCTCTAT ATTTTTCTCA 
    ATTCTCCGAT TTGATCCAAC GATTTCACAG ATCTCTGATC TAAATGGCAT 
    CCAACGGTGA TAAAGGACTG ATCGTTAGCT TCGGCGAGAT GCTAATCGAT 
    TTCGTCCCTA CCGAATCAGG CGTCTCTCTC GCCGAAGCTC CTGGATTCCT 
    CAAAGCTCCA GGCGGTGCTC CCGCTAACGT CGCAATCGCT GTTTCTCGTC 
    TCGGTGGACG ATCTGCTTTC GTCGGAAAAC TTGGAGACGA CGAGTTCGGT 
    CATATGTTGG CTGGGATTTT GAGGAAGAAC GGTGTTGATG ATCAGGGGAT 
    TAACTTCGAT ACTGGAGCTA GAACTGCTTT AGCTTTCGTT ACGTTACGTG 
    CTGATGGAGA TCGTGAGTTT ATGTTTTACC GCAATCCTAG CGCCGATATG 
    CTTCTTCGTC CTGATGAACT CAATCTCGAC CTCATCAGAT CCGCGAAAGT 
    GTTTCACTAT GGATCAATAA GTTTGATAGT GGAGCCGNGT AGGTCAGCTC 
    ATTTGAGGCC ATGGAAGTGG CGAAAGAAGC ANGAGCNCTT

    Gene models with high sequence identity

    1. AT2G31390.1 [+] show detail - pfkB-like carbohydrate kinase family protein
      E-value: 0; Score: 99.32

    3 prime EST [+] show sequence

    Nucleotide sequence: RAFL14-14-L14 - 3 prime EST 
    CCATAATTTA TTTTTATTAC TTCAATCTTA AAACATCTTA CACAAACACC 
    ACATACTGTA TATATGAGCA ACAAATAAAT GATAAAAATA AATAAAAACT 
    TGATTGAGTT ACAAAGGCAA TGCCAAGTAA CATATTCATG TATCAAAGAA 
    TCAAGAATTT AAGTGCACTC GGCAGGAACT CCTCGAGAAC CGTGGACGTT 
    GTTTCCTGTC GAAGTCGCAG ACTCGTCATC ACAGCTCAGG CGAGTAATTC 
    TGAGTTTCTT CTATCTACGC GCAATATCTC TCAAAGGAGG CCTCTTCAAA 
    GGTACCACAA TCATATCCTC TCGTTTCTAT CTGAAGAATC AGAGAGAAGA 
    GATATATAGA GGATGCCTGT GATCTCCAAA GCTTCATCTA CCACCACCAA 
    TTCATCGATT CCTTCGTGTT CTAGGATTGG AGG

    Gene models with high sequence identity

    1. AT2G43430.1 [+] show detail - glyoxalase 2-1
      E-value: 5e-133; Score: 100.00

    AT2G31390.1

    Model type
    Protein coding
    Short Description
    pfkB-like carbohydrate kinase family protein
    Computational Description
    pfkB-like carbohydrate kinase family protein; FUNCTIONS IN: kinase activity, ribokinase activity; INVOLVED IN: D-ribose metabolic process, acetate fermentation, sucrose biosynthetic process, sucrose catabolic process, using beta-fructofuranosidase; LOCATED IN: plasma membrane; EXPRESSED IN: 27 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: Carbohydrate/purine kinase (InterPro:IPR011611), Ribokinase (InterPro:IPR002139), Carbohydrate/puine kinase, PfkB, conserved site (InterPro:IPR002173); BEST Arabidopsis thaliana protein match is: pfkB-like carbohydrate kinase family protein (TAIR:AT1G06030.1); Has 18509 Blast hits to 18503 proteins in 2397 species: Archae - 386; Bacteria - 14053; Metazoa - 143; Fungi - 116; Plants - 466; Viruses - 0; Other Eukaryotes - 3345 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR

    AT2G43430.1

    Model type
    Protein coding
    Short Description
    glyoxalase 2-1
    Curator Summary
    Encodes a predicted mitochondrial glyoxalase GLX2-1. Studies using recombinant protein show that GLX2-1 contains a dinuclear metal binding site, but does not have glyoxalase 2 activity.
    Computational Description
    glyoxalase 2-1 (GLX2-1); CONTAINS InterPro DOMAIN/s: Beta-lactamase, class B, conserved site (InterPro:IPR001018), Beta-lactamase-like (InterPro:IPR001279), Hydroxyacylglutathione hydrolase (InterPro:IPR017782); BEST Arabidopsis thaliana protein match is: glyoxalase 2-4 (TAIR:AT1G06130.2); Has 15540 Blast hits to 15539 proteins in 2481 species: Archae - 339; Bacteria - 10160; Metazoa - 480; Fungi - 326; Plants - 230; Viruses - 0; Other Eukaryotes - 4005 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR