F12024

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F12024

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F12024

Determination of introduced cDNA(s)

  • RAFL06-74-I02 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL06-74-I02 - Resource: pda03502

    full reading [+] show sequence

    Nucleotide sequence: RAFL06-74-I02 - full reading 
    TGAAAGCGGT GAGTCATCTT ACCTTATAAA TGATCACAAA CCCGAGGACT 
    GTGAGTTTTC GTGTAGAACA AATCCTTCTC TGAGAAAAAA CAACAGATCC 
    GAATTTTATC TTTAATCAGC CGGAAAAAAT GGAGAAAGCG ATCGAGAGAC 
    AACGCGTTCT TCTTGAGCAT CTCCGACCTT CTTCTTCTTC TTCGCACAAT 
    TACGAGGCTT CTCTATCTGC TTCTGCTTGC TTGGCTGGGG ACAGTGCTGC 
    ATATCAGAGG ACCTCTCTCT ATGGAGATGA TGTTGTCATT GTCGCGGCAC 
    ATAGGACTCC ACTATGCAAG TCCAAACGTG GCAATTTCAA GGATACATAT 
    CCCGATGATT TGCTCGCACC TGTTTTGAGG GCATTGATAG AGAAGACGAA 
    TCTAAACCCA AGTGAAGTAG GTGACATTGT TGTGGGTACT GTTTTGGCAC 
    CTGGATCTCA GAGAGCCAGT GAATGCAGGA TGGCTGCGTT CTATGCTGGT 
    TTCCCTGAAA CCGTGGCTGT CAGAACTGTG AATAGACAGT GCTCATCTGG 
    GCTTCAGGCT GTTGCTGATG TAGCCGCTGC CATTAAAGCG GGATTTTATG 
    ACATTGGTAT CGGGGCTGGT TTGGAGTCCA TGACTACCAA TCCAATGGCA 
    TGGGAAGGGT CAGTCAACCC AGCGGTGAAG AAGTTTGCAC AAGCGCAGAA 
    TTGTCTTCTT CCTATGGGTG TTACGTCAGA AAATGTAGCA CAACGCTTTG 
    GTGTCTCAAG GCAGGAGCAA GATCAAGCTG CTGTTGACTC GCACAGAAAG 
    GCAGCTGCTG CTACTGCTGC TGGTAAATTC AAGGATGAGA TCATTCCTGT 
    TAAGACCAAG CTTGTTGACC CGAAGACTGG TGATGAGAAA CCCATTACAG 
    TTTCTGTTGA TGATGGTATC CGACCAACCA CAACTCTTGC TTCTCTTGGG 
    AAGCTGAAGC CAGTGTTTAA GAAGGATGGC ACCACTACTG CTGGAAATTC 
    CAGCCAAGTA AGTGATGGTG CAGGAGCGGT TCTCCTAATG AAGAGAAGTG 
    TTGCAATGCA AAAAGGACTT CCCGTTCTTG GTGTATTCAG GACATTTGCT 
    GCAGTTGGTG TTGACCCTGC AATCATGGGT ATCGGTCCAG CAGTTGCCAT 
    TCCTGCTGCA GTTAAGGCGG CTGGTTTAGA ACTTGATGAC ATCGACTTGT 
    TTGAGATCAA TGAGGCATTT GCATCTCAGT TTGTTTATTG CCGTAACAAA 
    TTGGGACTTG ACCCAGAGAA AATCAATGTC AACGGAGGTG CAATGGCCAT 
    AGGCCATCCT TTGGGCGCTA CAGGAGCGCG TTGTGTTGCT ACATTGTTGC 
    ACGAGATGAA ACGCCGTGGT AAAGACTGCC GTTTTGGAGT AGTGTCAATG 
    TGCATTGGGA CGGGGATGGG TGCAGCAGCT GTGTTTGAGA GAGGAGATGG 
    AGTTGATGAG CTTCGCAACG CAAGGAAAGT TGAAGCGCAA GGTCTTTTGT 
    CCAAGGACGC TCGCTAGAGA GGACCATGCA CCAAAACCGT TTTTCACCTC 
    ACCTCTCTCT TTTCTGGTCT TCTTGTCACT TTCTTCAGAT ATTATTATAG 
    TTTCGAATAA AGCACACAAC CAATGTTTGC CTGAGTCTTG TGTTGTTCTT 
    GACCAGGTCA TGTGTTTAGT ATTTTTTTTA AGTGAAGGGG GATAATCTTT 
    AAATCTTTTA TATGTAAGAA TATTGTTCTT CTCGAATTTC ATCAGTTGTT 
    GCC

    Gene models with high sequence identity

    1. AT2G33150.1 [+] show detail - peroxisomal 3-ketoacyl-CoA thiolase 3
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Thiolase, N-terminal 2.3e-83
    HMMPfam Thiolase, C-terminal 1.1e-48
    Gene3D Thiolase-like, subgroup 7.7e-63
    HMMTigr Thiolase 3.1e-204
    PatternScan Thiolase, acyl-enzyme intermediate active site 0
    PatternScan Thiolase, active site 0
    PatternScan Thiolase, conserved site 0
    superfamily Thiolase-like 6.8e-71
    superfamily Thiolase-like 3.1e-40
    HMMPanther 3-KETOACYL-COA THIOLASE B (BETA- KETOTHIOLASE 1.1e-226
    HMMPanther Thiolase 1.1e-226

    See the detailed result >

    AT2G33150.1

    Model type
    Protein coding
    Short Description
    peroxisomal 3-ketoacyl-CoA thiolase 3
    Curator Summary
    Encodes an organellar (peroxisome, glyoxysome) 3-ketoacyl-CoA thiolase, involved in fatty acid b-oxidation during germination and subsequent seedling growth. Mutants have defects in glyoxysomal fatty acid beta-oxidation. EC2.3.1.16 thiolase.
    Computational Description
    peroxisomal 3-ketoacyl-CoA thiolase 3 (PKT3); FUNCTIONS IN: acetyl-CoA C-acyltransferase activity; INVOLVED IN: fatty acid beta-oxidation, jasmonic acid biosynthetic process, response to wounding, fatty acid oxidation, glyoxysome organization; LOCATED IN: in 6 components; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Thiolase (InterPro:IPR002155), Thiolase, active site (InterPro:IPR020610), Thiolase, N-terminal (InterPro:IPR020616), Thiolase, conserved site (InterPro:IPR020613), Thiolase, C-terminal (InterPro:IPR020617), Thiolase-like, subgroup (InterPro:IPR016038), Thiolase-like (InterPro:IPR016039), Thiolase, acyl-enzyme intermediate active site (InterPro:IPR020615); BEST Arabidopsis thaliana protein match is: peroxisomal 3-ketoacyl-CoA thiolase 4 (TAIR:AT1G04710.1); Has 22382 Blast hits to 22371 proteins in 2261 species: Archae - 414; Bacteria - 14116; Metazoa - 985; Fungi - 655; Plants - 282; Viruses - 0; Other Eukaryotes - 5930 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR