F12226

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F12226

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1  
cauline leaf (PO:0000013)
  • decreased size (PATO:0000587)
    (Cauline leaf::Shape::small)
  • dark green (PATO:0001249)
    (Cauline leaf::Color::dark)
rosette leaf (PO:0000014)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)
  • increased size (PATO:0000586)
    (Rosette leaf::Shape::large)
sporophyte vegetative stage (PO:0007134)
  • decreased speed (PATO:0000304)
    (Adult plant::Growth::slow)
root (PO:0009005)
  • present in greater numbers in organism (PATO:0000470)
    (Root::Number::many)
stem (PO:0009047)
  • increased thickness (PATO:0000591)
    (Stem::Shape::thick)
  • increased thickness (PATO:0000591)
    (Stem::Shape::thick)
  • abnormal (PATO:0000460)
    (Stem::Others)
  • abnormal (PATO:0000460)
    (Stem::Others)
stem internode (PO:0020142)
  • decreased length (PATO:0000574)
    (Stem::Shape::short internode)
T2  
cauline leaf (PO:0000013)
  • increased size (PATO:0000586)
    (Cauline leaf::Shape::large)
  • dark green (PATO:0001249)
    (Cauline leaf::Color::dark)
  • abnormal (PATO:0000460)
    (Cauline leaf::Others)
rosette leaf (PO:0000014)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)
  • increased size (PATO:0000586)
    (Rosette leaf::Shape::large)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F12226

Determination of introduced cDNA(s)

  • RAFL09-13-B09 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL09-13-B09 - Resource: pda01703

    full reading [+] show sequence

    Nucleotide sequence: RAFL09-13-B09 - full reading
    GTCTTCCGTT GCGTGAGATA TTATTGAACG GTGTTTGTTA TTCGGAGAAG 
    AAGAAGAAGA AGAACTGAAG AAGCATATTC GACGGTCACC ACCAAACCTC
    TTCCGTCGGA TTTTCACTCT CCGTTTATCG AACATCTGAG ATCTCTCTCC
    ACTTCCGTCA CCGAAGATGC ATTCCAGTCA TCTTCTTCTC GAGGAGCCGA
    TCAGGATGGC TTCAATCCTC GAGCCTTCCA AATCTAGTTT CTTCCCGGCA
    TTGACTAAGA TCGTCGGAAC TCTAGGTCCT AAATCCCGAT CCGTCGAGGC
    TCTCTCCGGC TGTCTCAAAG CCGGCATGTC TGTGGCTCGA TTTGATTTCT
    CGTGGGGAGA TGCTGATTAT CACCAGGAGA CACTTGATAA TTTGAAAGTT
    GCTGTGAGGA GCACTAAGAA GCTTTGTGCT GTTATGCTTG ATACTGTTGG
    ACCTGAGCTA CAAGTTATTA ACAAATCTGA GAAAGCTATT ACTTTGAAAG
    CTGATGGCCT TGTAACTTTG ACACCGAATC AAGATCAAGA AGCTTCTTCT
    GAAGTTCTTC CCATTAATTT CAATGGACTT GCCAAGGCAG TGAAGAAAGG
    AGACACCATC TTCGTTGGGC AATACCTATT CACTGGTAGT GAAACAACTT
    CAGTTTGGCT CGAGGTTGAT GAAGTTAAAG GAGATGATGT CATTTGCCTT
    TCAAGGAATG CTGCTACTCT GGCTGGTTCT TTGTTCACTT TACACTCCTC
    TCAAGTTCAC ATTGATCTCC CAACGCTTAC AGAGAAGGAT AAGGAGGTTA
    TAAGCACATG GGGAGTTCAA AATAAAATCG ATTTTCTCTC ATTGTCTTAT
    TGTCGTCATG CTGAGGATGT TCGCCAGACC CGTGAAATGC TTAAAAAGTT
    GGGCGACCTC TCTCAAACAC AAATATTTGC TAAGATTGAG AACGTAGAGG
    GACTAACCCA CTTCGATGAG ATTCTACAAG AAGCTGATGG CATTATTCTT
    TCTCGTGGGA ATTTGGGTAT AGATTTACCC CCGGAAAAGG TGTTTTTGTT
    TCAAAAGGCT GCTCTTTACA AGTGCAACAT GGCTGGAAAG CCAGCTGTTC
    TTACCCGTGT TGTTGACAGT ATGACTGACA ACTTGCGACC AACTCGTGCT
    GAGGCCACGG ATGTTGCTAA TGCTGTTTTA GATGGAAGTG ATGCAATTCT
    TCTTGGTGCT GAGACCCTTC GTGGATTGTA CCCTGTTGAG ACAATATCAA
    CTGTTGGTAG GATCTGTGCT GAGGCAGAAA AGGTTTTCAA TCAAGATTTG
    TACTTTAAGA AGACTGTCAA GTATGTTGGA GAACCAATGA CTCACTTGGA
    ATCGATTGCT TCTTCAGCTG TACGGGCAGC CATTAAGGTT AAGGCATCCG
    TAATTATATG CTTCACCTCT TCTGGAAGAG CAGCCAGATT GATTGCCAAA
    TACAGGCCAA CGATGCCGGT TATTTCTGTT GTCATTCCCC GGGTTAAGAC
    AAATCAACTG AAATGGAGCT TTAGTGGAGC TTTTGAGGCG AGGCAGTCAC
    TTATTGTCAG AGGCCTCTTC CCTATGCTTG CTGACCCTCG TCACCCTGCG
    GAATCTACAA GTGCGACAAA CGAGTCAGTC CTGAAGGTTG CTCTAGACCA
    TGGGAAGCAT GCTGGAGTAA TCAAGTCGCA TGACAGAGTA GTGGTGTGTC
    AGAAAGTTGG TGATGCATCT GTGGTTAAGA TCATTGAGCT TGAGGATTAA
    CTTTTGCCTC GCAAAAACAT TATATAATTG GCTGCATTTT GGGTTGCCTT
    GAATTGATGA TAACTCCCAA TGCCGGAGTG AAAATATTAT CTCCGGGACT
    TCTGGTTTCT TTTTGTATAC TTAGGAGATT TGATGTCTCT AAGGTTTCAG
    CTATATTTGA TTACCTTCAA ATGTACGCAT ATGATAAAAG TATACAATGC
    CTAGCAATTT TATAATAAGC TGTTACATTC CCTT

    Gene models with high sequence identity

    1. AT3G52990.1 [+] show detail - Pyruvate kinase family protein
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    superfamily Pyruvate/Phosphoenolpyruvate kinase, catalytic core 6.6e-71
    superfamily Pyruvate kinase, C-terminal-like 4e-27
    superfamily Pyruvate kinase, beta-barrel-like 1.4e-08
    Gene3D Pyruvate/Phosphoenolpyruvate kinase, catalytic core 1.4e-66
    Gene3D Pyruvate kinase, alpha/beta 6.3e-18
    HMMPfam Pyruvate kinase, barrel 2.9e-93
    HMMPfam Pyruvate kinase, alpha/beta 8.2e-23
    HMMPanther Pyruvate kinase 5.2e-206
    HMMTigr Pyruvate kinase 2.5e-99
    FPrintScan Pyruvate kinase, barrel 1.2e-43
    FPrintScan Pyruvate kinase, barrel 1.2e-43
    FPrintScan Pyruvate kinase, barrel 1.2e-43
    FPrintScan Pyruvate kinase, barrel 1.2e-43
    FPrintScan Pyruvate kinase, barrel 1.2e-43
    FPrintScan Pyruvate kinase, barrel 1.2e-43

    See the detailed result >

    AT3G52990.1

    Model type
    Protein coding
    Short Description
    Pyruvate kinase family protein
    Computational Description
    Pyruvate kinase family protein; FUNCTIONS IN: pyruvate kinase activity, potassium ion binding, magnesium ion binding, catalytic activity; INVOLVED IN: response to cadmium ion, glycolysis; LOCATED IN: membrane; EXPRESSED IN: guard cell, cultured cell, leaf; EXPRESSED DURING: seedling growth; CONTAINS InterPro DOMAIN/s: Pyruvate/Phosphoenolpyruvate kinase, catalytic core (InterPro:IPR015813), Pyruvate kinase, alpha/beta (InterPro:IPR015794), Pyruvate kinase, beta-barrel-like (InterPro:IPR011037), Pyruvate kinase (InterPro:IPR001697), Pyruvate kinase, C-terminal-like (InterPro:IPR015795), Pyruvate kinase, barrel (InterPro:IPR015793); BEST Arabidopsis thaliana protein match is: Pyruvate kinase family protein (TAIR:AT2G36580.1); Has 9836 Blast hits to 9793 proteins in 2683 species: Archae - 167; Bacteria - 5933; Metazoa - 534; Fungi - 219; Plants - 549; Viruses - 0; Other Eukaryotes - 2434 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR