F12250

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F12250

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F12250

Determination of introduced cDNA(s)

  • RAFL05-14-B16 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-14-B16 - Resource: pdz87480

    5 prime EST [+] show sequence

    Nucleotide sequence: RAFL05-14-B16 - 5 prime EST 
    GTTGATCACT CGCTTTATAA ATCTCTCAGT TTCTTGCTCA CACCAACATC 
    TCTCTAAGCT TCTTCTTCTA CCAATCTAAT TCCTCTCTTC AGCTTCTTGT 
    GTTGTGACGC ATACTCGTCG CAGTCTTGAG ATATGGCCGC CGCAGTTTCC 
    ACCGTCGGTG CCATCAACAG AGCTCCGTTG AGCTTGAACG GGTCAGGATC 
    AGGAGCTGTA TCAGCCCCAG CTTCAACCTT CTTGGGAAAG AAAGTTGTAA 
    CTGTGTCGAG ATTCGCACAG AGCAACAAGA AGAGCAACGG ATCATTCAAG 
    GTGTTGGCTG TGAAAGAAGA CAAACAAACC GATGGAGACA GATGGAGAGG 
    TCTTGCCTAC GACACTTCTG ATGATCAACA AGACATCACC AGAGGCAAGG 
    GTATGGTTGA CTCTGTCTTC CAAGCTCCTA TGGGAACCGG AACTCACCAC 
    GCTGTCCTTA GCTCATACGA ATACGTTAGC CAAGGCCTTA GGCAGTACAA 
    CTTGGACAAC ATGATGGATG GGTTTTACAT TGCTCCTGCT TTCATGGACA 
    AGCTTGTTGT TCACATCACC AAGAACTTCT TGACTCTGCC TAACATCAAG 
    GTTCCACTTA TTTTGGGTAT ATGGGGAGGC AAAGGTCAAG GTAAATCCTT 
    NCAGTGTGAG CTTG

    Gene models with high sequence identity

    1. AT2G39730.3 [+] show detail - rubisco activase
      E-value: 0; Score: 99.85

    3 prime EST [+] show sequence

    Nucleotide sequence: RAFL05-14-B16 - 3 prime EST 
    AGGAATAAGA AGTGTAGTTT ATGACAAGGC AATTTTAAAT TACCAATGTG 
    TTGTACATCA TAAAAAAAAA AAAAGGGAAC AAAAGAGGAC AAGATCCAAT 
    CCACAATTAC ACAAAGGAGA AAAATAAACC AAAAGAGACA ATAGGAGTAA 
    AACAGCAAAT TAATAAGCAG GATAATATTG CTCAAAAGTT GTAGACACAG 
    GTTCCATCGT CACTTCTAGC CGTTGGATCA AAGTTTTCAG CCACAGGATC 
    AGTACACCCT TCAGGAACTG GCAGGTTTAC TTGCTGGGCT CCTTTTCCTC 
    TGTTTTACCG TA

    Gene models with high sequence identity

    1. AT2G39730.2 [+] show detail - rubisco activase
      E-value: 3e-176; Score: 100.00

    AT2G39730.2

    Model type
    Protein coding
    Short Description
    rubisco activase
    Curator Summary
    Rubisco activase, a nuclear-encoded chloroplast protein that consists of two isoforms arising from alternative splicing in most plants. Required for the light activation of rubisco.
    Computational Description
    rubisco activase (RCA); FUNCTIONS IN: enzyme regulator activity, ADP binding, ribulose-1,5-bisphosphate carboxylase/oxygenase activator activity, ATP binding; INVOLVED IN: response to cold, response to light stimulus, defense response to bacterium; LOCATED IN: in 10 components; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: ATPase, AAA-type, core (InterPro:IPR003959); BEST Arabidopsis thaliana protein match is: P-loop containing nucleoside triphosphate hydrolases superfamily protein (TAIR:AT1G73110.1); Has 2331 Blast hits to 2283 proteins in 512 species: Archae - 328; Bacteria - 459; Metazoa - 248; Fungi - 427; Plants - 404; Viruses - 0; Other Eukaryotes - 465 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR

    AT2G39730.3

    Model type
    Protein coding
    Short Description
    rubisco activase
    Curator Summary
    Rubisco activase, a nuclear-encoded chloroplast protein that consists of two isoforms arising from alternative splicing in most plants. Required for the light activation of rubisco.
    Computational Description
    rubisco activase (RCA); FUNCTIONS IN: enzyme regulator activity, ADP binding, ribulose-1,5-bisphosphate carboxylase/oxygenase activator activity, ATP binding; INVOLVED IN: response to cold, response to light stimulus, defense response to bacterium; LOCATED IN: in 10 components; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: ATPase, AAA-type, core (InterPro:IPR003959); BEST Arabidopsis thaliana protein match is: P-loop containing nucleoside triphosphate hydrolases superfamily protein (TAIR:AT1G73110.1); Has 2322 Blast hits to 2274 proteins in 510 species: Archae - 328; Bacteria - 451; Metazoa - 248; Fungi - 425; Plants - 405; Viruses - 0; Other Eukaryotes - 465 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR