F12311

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F12311

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
cauline leaf (PO:0000013)
  • decreased width (PATO:0000599)
    (Cauline leaf::Shape::narrow)
  • epinastic (PATO:0000945)
    (Cauline leaf::Shape::epinastic)
  • dark green (PATO:0001249)
    (Cauline leaf::Color::dark)
rosette leaf (PO:0000014)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)
  • decreased width (PATO:0000599)
    (Rosette leaf::Shape::narrow)
stem (PO:0009047)
  • increased thickness (PATO:0000591)
    (Stem::Shape::thick)
  • abnormal (PATO:0000460)
    (Stem::Others)
  • abnormal (PATO:0000460)
    (Stem::Others)
T2  
cauline leaf (PO:0000013)
  • increased size (PATO:0000586)
    (Cauline leaf::Shape::large)
rosette leaf (PO:0000014)
  • increased size (PATO:0000586)
    (Rosette leaf::Shape::large)
  • epinastic (PATO:0000945)
    (Rosette leaf::Shape::epinastic)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
fruit (PO:0009001)
  • increased thickness (PATO:0000591)
    (Silique::Shape::thick)
stem (PO:0009047)
  • increased thickness (PATO:0000591)
    (Stem::Shape::thick)
  • abnormal (PATO:0000460)
    (Stem::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F12311

Determination of introduced cDNA(s)

  • RAFL06-69-M11 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL06-69-M11 - Resource: pda03330

    full reading [+] show sequence

    Nucleotide sequence: RAFL06-69-M11 - full reading 
    AAGAAGTAGC AGAACAATGG CGACTTCTCA GCAATTTTTA AACCCTACAC 
    TCTTCAAATC CTTAGCTTCC TCAAACAAAA ACTCATGTAC TCTCTGCCCA 
    TCTCCTTTCT TGCAACTCAA GTCCGCCTCC ACAATTTTCA ATTACAAACC 
    ACTTACTTCC TCCTCCGCCA CGATCATCAC ACGCGTCGCT GCATCATCCT 
    CCGATTCAGG CGAGTCAATA ACCAGAGAGA CTTTCCACGG CCTCTGCTTC 
    GTCTTGAAAG ACAACATCGA CACCGATCAA ATAATCCCCG CCGAGTACGG 
    CACTCTCATC CCTTCGATTC CAGAAGATCG CGAGAAACTC GGCTCTTTCG 
    CGCTTAACGG CTTACCAAAA TTCTACAACG AACGTTTCGT TGTTCCAGGA 
    GAGATGAAAT CAAAGTACTC AGTCATCATC GGCGGCGATA ATTTCGGTTG 
    CGGATCTTCC CGCGAACACG CTCCAGTTTG TCTCGGCGCG GCGGGAGCTA 
    AAGCTGTGGT GGCGGAATCG TACGCTAGGA TCTTTTTCAG GAACTGTGTA 
    GCTACAGGTG AGATTTTCCC GTTGGAATCG GAGGTTAGGA TTTGCGACGA 
    GTGCAAAACA GGGGATGTGG TGACAATCGA ACACAAGGAA GACGGTAGTA 
    GTTTGCTGAT CAATCATACG ACGAGGAAAG AATACAAACT GAAACCGCTC 
    GGTGATGCCG GTCCGGTGAT CGACGCCGGT GGAATCTTCG CTTATGCAAG 
    AAAAGCCGGC ATGATTCCTT CTGCTTGAAT GTAATCGGAT CCATAATTTA 
    TCGGTTCTGA GTTTAATCCG GTTTGGTTTA TTTGGTTCGA GAACCGGTAC 
    TAACGAAATA AATTATTACC AAACCAAAAT TTCTTCTGGT TCGACTTTGT 
    TTGCCACGTG GAAACAGCGA TATAAACAAG TGCCCAAAAA GGCTTGCAAT 
    GGTTTTTGTA AAAAAAAAAA AAAAA

    Gene models with high sequence identity

    1. AT3G58990.1 [+] show detail - isopropylmalate isomerase 1
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Aconitase A/isopropylmalate dehydratase small subunit, swivel 4.9e-18
    Gene3D Aconitase/3-isopropylmalate dehydratase, swivel 4.2e-26
    HMMPanther 3-isopropylmalate dehydratase, small subunit-like 1.1e-52
    HMMPanther Aconitase-like core 1.1e-52
    superfamily Aconitase/3-isopropylmalate dehydratase, swivel 3.1e-38

    See the detailed result >

    AT3G58990.1

    Model type
    Protein coding
    Short Description
    isopropylmalate isomerase 1
    Computational Description
    isopropylmalate isomerase 1 (IPMI1); FUNCTIONS IN: hydro-lyase activity, 3-isopropylmalate dehydratase activity; INVOLVED IN: leucine biosynthetic process, metabolic process; LOCATED IN: plastid; EXPRESSED IN: 19 plant structures; EXPRESSED DURING: 9 growth stages; CONTAINS InterPro DOMAIN/s: 3-isopropylmalate dehydratase, small subunit-like (InterPro:IPR012305), Aconitase A/isopropylmalate dehydratase small subunit, swivel (InterPro:IPR000573), Aconitase/3-isopropylmalate dehydratase, swivel (InterPro:IPR015928), Aconitase-like core (InterPro:IPR015937); BEST Arabidopsis thaliana protein match is: Aconitase/3-isopropylmalate dehydratase protein (TAIR:AT2G43090.1); Has 8940 Blast hits to 8939 proteins in 2288 species: Archae - 372; Bacteria - 5509; Metazoa - 6; Fungi - 358; Plants - 71; Viruses - 0; Other Eukaryotes - 2624 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR