F12547

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F12547

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
cauline leaf (PO:0000013)
  • decreased width (PATO:0000599)
    (Cauline leaf::Shape::narrow)
  • epinastic (PATO:0000945)
    (Cauline leaf::Shape::epinastic)
fruit (PO:0009001)
  • abnormal (PATO:0000460)
    (Silique::Others)
stem (PO:0009047)
  • dark green (PATO:0001249)
    (Stem::Color::dark)
  • abnormal (PATO:0000460)
    (Stem::Others)
T2  
cauline leaf (PO:0000013)
  • decreased width (PATO:0000599)
    (Cauline leaf::Shape::narrow)
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • upturned (PATO:0002031)
    (Rosette leaf::Shape::hyponastic)
fruit (PO:0009001)
  • abnormal (PATO:0000460)
    (Silique::Other)
stem (PO:0009047)
  • decreased thickness (PATO:0000592)
    (Stem::Shape::thin)
  • abnormal (PATO:0000460)
    (Stem::Others)
  • abnormal (PATO:0000460)
    (Stem::Shape::zigzag)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F12547

Determination of introduced cDNA(s)

  • RAFL09-74-G13 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL09-74-G13 - Resource: pda08743

    full reading [+] show sequence

    Nucleotide sequence: RAFL09-74-G13 - full reading
    ATTCTCTCTC TAACTTCTCT ATGTTAAAAT TCCGAAAGCT CGATTCAGAA 
    ATCTAGAAGC TTCCTCGAGG GCCCTCGCGT AGAAAGTCGA ACTCAAATTC
    CCTGGAGACA AAGAAGAGGA AGATGAGCTC ATGGAACTAC GTTGTTACGG
    CTCACAAACC GACCAGTGTC ACTCACTCGT GCGTTGGCAA CTTCACAAGT
    CCTCAAGAGC TCAATCTCAT TGTCGCGAAA TGTACTCGAA TCGAGATCCA
    TTTGCTTACC CCACAAGGAC TTCAGCCTAT GTTGGACGTT CCTATATATG
    GAAGAATCGC TACTCTGGAG TTGTTCCGAC CCCATGGCGA AGCGCAAGAC
    TTTTTGTTCA TTGCAACTGA GAGATACAAA TTCTGTGTTC TTCAATGGGA
    TCCTGAGTCC TCTGAGCTTA TTACAAGAGC AATGGGGGAT GTTTCTGATC
    GTATAGGCCG GCCAACAGAC AATGGTCAGA TTGGTATAAT TGATCCAGAT
    TGTAGATTAA TTGGACTCCA TCTGTATGAC GGCTTGTTCA AGGTTATTCC
    ATTTGATAAT AAGGGACAGC TCAAGGAAGC TTTTAATATC AGGCTTGAGG
    AATTGCAGGT TCTGGATATC AAGTTTCTGT TTGGATGTGC AAAGCCCACT
    ATTGCAGTAC TATATCAGGA CAACAAAGAT GCTCGTCATG TCAAAACATA
    TGAAGTCTCT CTTAAAGATA AGGATTTTGT CGAGGGTCCA TGGTCTCAGA
    ACAGTCTCGA CAATGGTGCT GACTTGCTGA TTCCTGTACC ACCACCGCTC
    TGTGGTGTCC TTATTATTGG TGAAGAAACA ATTGTCTATT GCAGTGCTAG
    TGCATTCAAA GCAATACCAA TAAGACCTTC CATCACTAAA GCATATGGAA
    GAGTTGATGT TGATGGCTCT AGATATCTTC TTGGTGACCA TGCTGGAATG
    ATTCATCTGC TTGTTATAAC GCACGAGAAA GAAAAGGTCA CTGGCCTAAA
    AATTGAGCTT TTGGGTGAAA CATCTATTGC ATCGACTATA TCGTACCTTG
    ACAATGCCGT TGTCTTTGTC GGCTCAAGCT ATGGTGATTC ACAGCTAGTA
    AAGCTGAATC TACACCCTGA TGCAAAAGGC TCATATGTAG AAGTCTTGGA
    AAGGTATATC AACTTGGGGC CTATTGTCGA CTTTTGTGTA GTTGACCTCG
    AGAGACAGGG GCAAGGTCAG GTTGTAACTT GTTCTGGAGC ATTTAAGGAT
    GGTTCCCTCC GCGTAGTTCG CAATGGAATA GGAATCAATG AACAGGCGTC
    TGTGGAACTT CAAGGTATCA AAGGAATGTG GTCGTTGAAA TCTTCAATTG
    ATGAAGCCTT CGATACATTT CTTGTTGTTA GCTTTATCAG TGAAACTCGC
    ATATTAGCCA TGAATCTTGA GGATGAACTG GAAGAAACAG AGATTGAGGG
    CTTCTTGTCT CAAGTGCAGA CGTTATTTTG CCATGATGCT GTGTACAATC
    AACTTGTACA GGTTACCTCA AATTCTGTTA GACTAGTCAG TTCTACAACT
    AGAGAATTGC GGGACGAGTG GCATGCCCCA GCTGGATTCA CTGTTAATGT
    TGCAACTGCA AATGCCAGCC AGGTTCTTTT GGCGACTGGA GGTGGGCATT
    TGGTTTATCT AGAAATTGGA GATGGGAAAT TGACGGAAGT GCAACATGCC
    CTTTTGGAGT ACGAGTTTTC CTGTCTTGAT ATAAACCCTA TCGGTGACAA
    TCCTAATTAC AGTCAGCTAG CTGCGGTTGG GATGTGGACA GATATAAGTG
    TGAGGATCTT TTCGCTGCCA GAATTGACTC TTATTACAAA AGAGCAACTT
    GGAGGGGAAA TAATTCCCCG ATCTGTTCTT CTTTGTGCAT TCGAAGGGAT
    ATCTTACTTG CTATGTGCTC TTGGAGATGG CCATCTTTTG AACTTCCAGA
    TGGATACAAC TACCGGACAA TTAAAAAAAA AAAAAAAA

    Gene models with high sequence identity

    1. AT4G05420.1 [+] show detail - damaged DNA binding protein 1A
      E-value: 0; Score: 99.95

     

    InterPro Scan Digest

    Program Description E-value
    Coil coiled-coil 0
    HMMPanther DNA REPAIR PROTEIN XP-E 0
    HMMPanther DNA REPAIR/RNA PROCESSING CPSF FAMILY 0

    See the detailed result >

    AT4G05420.1

    Model type
    Protein coding
    Short Description
    damaged DNA binding protein 1A
    Curator Summary
    Structurally similar to damaged DNA binding proteins.DDB1a is part of a 350 KDa nuclear localized DET1 protein complex. This complex may physically interact with histone tails and while bound to chromatin- repress transcription of genes involved in photomorphogenesis.
    Computational Description
    damaged DNA binding protein 1A (DDB1A); FUNCTIONS IN: protein binding, DNA binding; INVOLVED IN: negative regulation of transcription, negative regulation of photomorphogenesis; LOCATED IN: nucleus, CUL4 RING ubiquitin ligase complex; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: WD40 repeat-like-containing domain (InterPro:IPR011046), Cleavage/polyadenylation specificity factor, A subunit, C-terminal (InterPro:IPR004871); BEST Arabidopsis thaliana protein match is: damaged DNA binding protein 1B (TAIR:AT4G21100.1); Has 1490 Blast hits to 1182 proteins in 227 species: Archae - 0; Bacteria - 0; Metazoa - 604; Fungi - 374; Plants - 231; Viruses - 0; Other Eukaryotes - 281 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR