F12934

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T2	seed (PO:0009010)	increased size (PATO:0000586) (Seed::Shape::large)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F12934

Determination of introduced cDNA(s)

RAFL09-10-I20 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL09-10-I20 - Resource: pda17635

full reading [+] show sequence

CCCCCGACTG CTCCCAAGTC CTCTCACTTC TTCTTCTTCC TCCGACACCG 
TTGCTTAACG GTTTTCCATT TTTGTCCCTT CAAGATGATC TCATGGCACG 
ACCTCTACAC GGTCCTCACC GCCGTGATTC CTCTCTACGT GGCCATGATC 
CTCGCTTACG GCTCTGTCCG GTGGTGGAAA ATCTTCTCAC CCGACCAATG 
CTCCGGAATC AACCGTTTTG TCGCCATCTT CGCCGTCCCT CTCCTCTCTT 
TTCACTTCAT CTCCACCAAC AATCCTTACG CAATGAATCT CCGGTTCATC 
GCCGCCGACA CTCTCCAAAA AATCATCATG TTGTCACTTT TAGTCCTATG 
GGCTAATTTC ACTCGCTCCG GTAGCCTCGA GTGGAGCATC ACAATCTTTT 
CCCTCTCCAC ACTTCCCAAC ACTCTTGTTA TGGGGATTCC TCTCTTGATC 
GCCATGTATG GCGAATACTC TGGTTCCCTC ATGGTCCAAA TCGTCGTCCT 
CCAGTGTATC ATCTGGTACA CGCTTCTCCT TTTTCTCTTC GAGTTTCGTG 
GCGCCAAGAT GCTCATCATG GAGCAGTTCC CTGAGACGGC TGCTTCCATT 
GTTTCTTTCA AAGTCGAATC CGACGTCGTT TCGCTCGACG GCCATGATTT 
TCTTGAGACC GATGCAGAGA TAGGTGACGA CGGGAAGCTT CACGTCACCG 
TGAGAAAATC CAACGCTTCA CGTCGTTCGT TCTGCGGCCC GAACATGACT 
CCACGGCCGT CAAATCTCAC CGGAGCTGAG ATTTATAGTC TCAGCACCAC 
TCCTAGAGGC TCTAATTTCA ACCACTCTGA TTTTTACAAC ATGATGGGTT 
TCCCCGGTGG TCGTCTCTCC AATTTCGGTC CGGCGGATAT GTACTCCGTT 
CAATCATCTA GAGGTCCAAC TCCTCGACCT TCAAACTTCG AGGAGAATTG 
CGCCATGGCA TCCTCCCCGA GATTCGGGTA TTACCCTGGA GGAGGAGCCG 
GGTCTTATCC GGCTCCGAAT CCAGAGTTCT CTTCAACCAC CACATCTACC 
GCCAATAAAA GCGTCAATAA AAACCCGAAA GACGTTAATA CGAATCAGCA 
GACGACTCTT CCAACGGGCG GCAAGTCAAA CAGCCATGAC GCCAAGGAGC 
TTCACATGTT CGTCTGGAGC TCAAACGGGT CACCCGTTTC AGACCGGGCG 
GGTCTTAACG TTTTCGGCGG AGCACCTGAC AACGATCAAG GCGGAAGATC 
TGACCAAGGT GCTAAAGAGA TCCGTATGTT AGTCCCAGAT CAATCTCACA 
ACGGCGAGAC CAAAGCTGTA GCTCATCCAG CAAGTGGAGA TTTCGGAGGA 
GAACAACAAT TTAGTTTCGC CGGAAAAGAA GAAGAAGCAG AGAGACCAAA 
AGACGCCGAG AATGGTCTAA ACAAACTTGC TCCAAATTCC ACGGCGGCGC 
TACAATCCAA GACAGGTCTA GGAGGAGCCG AAGCAAGTCA ACGAAAAAAT 
ATGCCTCCGG CGAGTGTGAT GACAAGGCTG ATACTGATAA TGGTTTGGAG 
GAAACTCATC AGAAACCCAA ACACTTACTC TAGTCTCATT GGACTTATTT 
GGGCTCTCGT CGCTTTCCGG TGGCACGTGG CAATGCCCAA AATCATTCAA 
CAATCTATCT CCATTCTATC TGATGCTGGT CTTGGAATGG CAATGTTTAG 
TTTGGGGTTG TTCATGGCGT TGCAACCCAA ATTAATCGCT TGTGGGAATT 
CAGTGGCAAC GTTTGCCATG GCGGTTAGGT TCCTTACGGG TCCGGCCGTG 
ATGGCGGTTG CTGCTATAGC CATCGGATTA CGTGGTGATT TACTGCGTGT 
CGCTATAGTT CAGGCCGCAT TACCTCAAGG AATTGTGCCC TTTGTGTTTG 
CGAAGGAGTA CAATGTTCAT CCTGCTATTT TAAGTACAGG GGTAATATTT 
GGAATGCTTA TAGCGCTTCC GATCACGCTG GTTTACTACA TTCTACTCGG 
GTTATAAGCT TTCGGTTATG AAAGCCCCGA GAAAGAGTCA ATTGTAGAGG 
GAGAAGGAAG AAAGGGAAAC TAGAGAGTGT CTCATGGAAC AAACTAAAAA 
AAGCTTAGAG TATGTAAGTA CAACTAGGGA GTAGCATGAT TTAAGGAATG 
CTGATGCCAA CATTACAAGC CAAGAAACCC TTTCTTCTCC CATATTTTTT 
ATATATAGAA TATTGGGAGA TTATTTGAGT GAGACAATGG GACGCAGATA 
AGGAGGAGCA AAACATGGGG CAACGAGACA AAGAAAAGAT AAATAAAAGA 
TTGGAAGATG AAGACAACTT ATTATTTTTG TAACTTTTAT TTTTCTTACA 
CAGTCTTTTT TTTTTTTTTT TGTGTTCTTT GATTAGGTTC GGGTAACTCT 
TAGTTGATTA TTGTGTAGAT TTTTTTTAGC GAAGAAAAGT GTGGTTTTGA 
TTTTGAACAA AAAAA

Gene models with high sequence identity

AT1G70940.1 [+] show detail - Auxin efflux carrier family protein
E-value: 0; Score: 99.96

InterPro Scan Digest

Program	Description	E-value
HMMTigr	Auxin efflux carrier, subgroup	0
Coil	coiled-coil	0
HMMPfam	Auxin efflux carrier	6.7e-187

See the detailed result >

AT1G70940.1

Model type: Protein coding
Short Description: Auxin efflux carrier family protein
Curator Summary: A regulator of auxin efflux and involved in differential growth. PIN3 is expressed in gravity-sensing tissues, with PIN3 protein accumulating predominantly at the lateral cell surface. PIN3 localizes to the plasma membrane and to vesicles. In roots, PIN3 is expressed without pronounced polarity in tiers two and three of the columella cells, at the basal side of vascular cells, and to the lateral side of pericycle cells of the elongation zone. PIN3 overexpression inhibits root cell growth. Protein phosphorylation plays a role in PIN3 trafficking to the plasma membrane.
Computational Description: PIN-FORMED 3 (PIN3); FUNCTIONS IN: auxin:hydrogen symporter activity, transporter activity; INVOLVED IN: in 8 processes; LOCATED IN: lateral plasma membrane, plasma membrane, vesicle membrane, cell surface; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Auxin efflux carrier, subgroup (InterPro:IPR014024), Auxin efflux carrier (InterPro:IPR004776); BEST Arabidopsis thaliana protein match is: Auxin efflux carrier family protein (TAIR:AT1G23080.1); Has 1983 Blast hits to 1757 proteins in 550 species: Archae - 37; Bacteria - 1198; Metazoa - 14; Fungi - 0; Plants - 487; Viruses - 0; Other Eukaryotes - 247 (source: NCBI BLink).
Link: InterPro Scan - TAIR