F13219

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F13219

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
cauline leaf (PO:0000013)
  • decreased size (PATO:0000587)
    (Cauline leaf::Shape::small)
  • desaturated green (PATO:0001272)
    (Cauline leaf::Color::pale)
  • abnormal (PATO:0000460)
    (Cauline leaf::Others)
rosette leaf (PO:0000014)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)
  • decreased width (PATO:0000599)
    (Rosette leaf::Shape::narrow)
fruit (PO:0009001)
  • abnormal (PATO:0000460)
    (Silique::Others)
stem (PO:0009047)
  • present in fewer numbers in organism (PATO:0001997)
    (Stem::Number::few)
  • abnormal (PATO:0000460)
    (Stem::Others)
stem internode (PO:0020142)
  • decreased length (PATO:0000574)
    (Stem::Shape::short internode)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F13219

Determination of introduced cDNA(s)

  • RAFL05-05-M23 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-05-M23 - Resource: pda01064

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-05-M23 - full reading 
    ATTGTCGTCG GAGAAGGTGA AACAGCTCCG GTTGGTGCTG CGATTGGATT 
    GTTAGCTGAG ACTGAAGCTG AGATCGAAGA AGCTAAGAGT AAAGCCGCTT 
    CGAAATCTTC TTCTTCTGTG GCTGAGGCTG TCGTTCCATC TCCTCCTCCG 
    GTTACTTCTT CTCCTGCTCC GGCGATTGCT CAACCGGCTC CGGTGACGGC 
    AGTATCAGAT GGTCCGAGGA AGACTGTTGC GACGCCGTAT GCTAAGAAGC 
    TTGCTAAACA ACACAAGGTT GATATTGAAT CCGTTGCTGG AACTGGACCA 
    TTCGGTAGGA TTACGGCTTC TGATGTGGAG ACGGCGGCTG GAATTGCTCC 
    GTCCAAATCC TCCATCGCAC CACCGCCTCC TCCTCCACCT CCGGTGACGG 
    CTAAAGCAAC CACCACTAAT TTGCCTCCTC TGTTACCTGA TTCAAGCATT 
    GTTCCTTTCA CAGCAATGCA ATCTGCAGTA TCTAAGAACA TGATTGAGAG 
    TCTCTCTGTT CCTACATTCC GTGTTGGTTA TCCTGTGAAC ACTGACGCTC 
    TTGATGCACT TTACGAGAAG GTGAAGCCAA AGGGTGTAAC AATGACAGCT 
    TTATTAGCTA AAGCTGCAGG GATGGCCTTG GCTCAGCATC CTGTGGTGAA 
    CGCTAGCTGC AAAGACGGGA AGAGTTTTAG TTACAATAGT AGCATTAACA 
    TTGCAGTGGC GGTTGCTATC AATGGTGGCC TGATTACGCC TGTTCTACAA 
    GATGCAGATA AGTTGGATTT GTACTTGTTA TCTCAAAAAT GGAAAGAGCT 
    GGTGGGGAAA GCTAGAAGCA AGCAACTTCA ACCCCATGAA TACAACTCTG 
    GAACTTTTAC TTTATCGAAT CTCGGTATGT TTGGAGTGGA TAGATTTGAC 
    GCTATTCTTC CGCCAGGACA GGGTGCTATT ATGGCTGTTG GAGCGTCAAA 
    GCCAACTGTA GTTGCTGATA AGGATGGATT CTTCAGTGTA AAAAACACAA 
    TGCTGGTGAA TGTGACTGCA GATCATCGCA TTGTGTATGG AGCTGACTTG 
    GCTGCTTTTC TCCAAACCTT TGCAAAGATC ATTGAGAATC CAGATAGTTT 
    GACCTTATAA GACGCCAAGC GAAGACGAGA AGTCAAAAAC AGTTTCCAAA 
    ATTCCTGAGC CAAATTTTTC CCAAGTAAAT TTTTTAATCT TCATTGTTCT 
    TGGTCTTGCT CTACTTCTTT TGCATCTTTT TCTTCACTTG TGTTGTATCT 
    GTATTTTTGT TTTCAAGAAT CATCATTTTG GGTTTTAAAC AAAAAAAAAA 
    AAAAAAAAAA AAAAAAAAAA ACAAAAAAAA AAAAAAAA

    Gene models with high sequence identity

    1. AT3G25860.1 [+] show detail - 2-oxoacid dehydrogenases acyltransferase family protein
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPanther PTHR23151 0
    Gene3D E3 binding 0.0000489999951782359
    Gene3D G3DSA:3.30.559.10 2.30000440726539e-60
    HMMPfam 2-oxoacid dehydrogenase acyltransferase, catalytic domain 5.30000000000001e-64
    superfamily SSF52777 1.2e-66
    superfamily E3 binding 0.000000000062
    HMMPfam E3 binding 0.000000000026
    HMMPanther PTHR23151:SF9 0

    See the detailed result >

    AT3G25860.1

    Model type
    Protein coding
    Short Description
    2-oxoacid dehydrogenases acyltransferase family protein
    Curator Summary
    Nuclear encoded dihydrolipoamide S-acetyltransferase (LTA2) that encodes teh Pyruvate Decarboxylase E2 subunit. Mutant has embryo defect.
    Computational Description
    LTA2; FUNCTIONS IN: dihydrolipoyllysine-residue acetyltransferase activity; INVOLVED IN: metabolic process, acetyl-CoA biosynthetic process from pyruvate; LOCATED IN: cytosolic ribosome, chloroplast stroma, chloroplast, membrane, chloroplast envelope; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: 2-oxo acid dehydrogenase, lipoyl-binding site (InterPro:IPR003016), E3 binding (InterPro:IPR004167), 2-oxoacid dehydrogenase acyltransferase, catalytic domain (InterPro:IPR001078), Single hybrid motif (InterPro:IPR011053), Biotin/lipoyl attachment (InterPro:IPR000089); BEST Arabidopsis thaliana protein match is: 2-oxoacid dehydrogenases acyltransferase family protein (TAIR:AT1G34430.1); Has 23844 Blast hits to 20819 proteins in 2344 species: Archae - 101; Bacteria - 13163; Metazoa - 1146; Fungi - 799; Plants - 467; Viruses - 32; Other Eukaryotes - 8136 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR