F13249

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F13249

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
cauline leaf (PO:0000013)
  • epinastic (PATO:0000945)
    (Cauline leaf::Shape::epinastic)
rosette leaf (PO:0000014)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
sporophyte vegetative stage (PO:0007134)
  • decreased speed (PATO:0000304)
    (Adult plant::Growth::slow)
stem (PO:0009047)
  • abnormal (PATO:0000460)
    (Stem::Others)
  • present in fewer numbers in organism (PATO:0001997)
    (Stem::Number::few)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F13249

Determination of introduced cDNA(s)

  • RAFL09-91-M24 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL09-91-M24 - Resource: pda17905

    full reading [+] show sequence

    Nucleotide sequence: RAFL09-91-M24 - full reading 
    CCTGAAGACA AAGCGCAGAA AATGGTGGGA GCGACTGTAG TTAGTAAATG 
    GACTCCCCTA TGTGTGGCTT CGCCGCCGGA GAGAAACTCG GCAAGTCTCA 
    ATCCACACTG TTCTCCAGCC AGGGTTAATT TTAGAACAGC GTTGGCCGCA 
    TTTCGTCCTC AGTTCCGTCT TTTCTCTCGC AATTCCGCGT CTCGCCGCCG 
    TCTTCGCGCT TCCAGCTCCG CCGAATCGGG AATCTTCCTT CCTCACCTTG 
    TAGCTTCTAT GGAGGACGTT GAGGAGACTT ACATTATGGT GAAACCTGAT 
    GGCATACAAC GAGGCCTTGT AGGAGAAATC ATTTCTCGCT TTGAGAAAAA 
    GGGGTTTAAA CTTATTGGGC TCAAGATGTT TCAGTGCCCA AAAGAATTGG 
    CTGAGGAGCA TTATAAGGAT CTTAGTGCTA AATCATTCTT TCCTAACCTG 
    ATTGAGTACA TCACTTCAGG TCCAGTTGTG TGTATGGCTT GGGAAGGTGT 
    TGGTGTTGTT GCTTCAGCCA GGAAGCTAAT AGGGAAAACA GATCCGCTTC 
    AAGCTGAACC TGGTACTATA AGAGGAGATC TTGCTGTGCA AACCGGAAGG 
    AACATTGTGC ATGGTAGTGA CAGCCCTGAA AACGGCAAGC GTGAGATTGG 
    TCTGTGGTTC AAAGAGGGCG AGCTATGCAA GTGGGATTCG GCTCTAGCTA 
    CATGGCTAAG GGAGTGATCC TTGAGAAGAC AGAGTCAATT ATAATGGTGT 
    CCTTACTTGT TTTCTTGTTA CCGATGATGA GCTTTGGTGT TTTGTTCCTG 
    ATTTTGGGCG GCAATATAAT ATAACTGCCA ATCTATGGCT TGATTCTTCT 
    CATATCTGTG ATCACTTGTC ATGTAATCAA AACTAGTTAC ACAAAAAAAA 
    AA

    Gene models with high sequence identity

    1. AT5G63310.1 [+] show detail - nucleoside diphosphate kinase 2
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMSmart Nucleoside diphosphate kinase, core 4.6e-86
    superfamily Nucleoside diphosphate kinase, core 6.2e-57
    HMMPfam Nucleoside diphosphate kinase, core 1.3e-54
    HMMPanther Nucleoside diphosphate kinase, core 7.3e-31
    PatternScan Nucleoside diphosphate kinase, core 0
    FPrintScan Nucleoside diphosphate kinase, core 1.1e-49
    FPrintScan Nucleoside diphosphate kinase, core 1.1e-49
    FPrintScan Nucleoside diphosphate kinase, core 1.1e-49
    FPrintScan Nucleoside diphosphate kinase, core 1.1e-49
    FPrintScan Nucleoside diphosphate kinase, core 1.1e-49
    Gene3D Nucleoside diphosphate kinase, core 3e-55
    HAMAP Nucleoside diphosphate kinase, core 33.18

    See the detailed result >

    AT5G63310.1

    Model type
    Protein coding
    Short Description
    nucleoside diphosphate kinase 2
    Curator Summary
    Maintains intracellular dNTP levels except ATP. Plays a role in response to oxidative stress and UV. Involved in phytochrome-mediated light signaling. Participates in auxin-regulated processes, partly through the modulation of auxin transport. H-bonding with His-197 inside the nucleotide-binding pocket is critical for NDPK2 functioning.
    Computational Description
    nucleoside diphosphate kinase 2 (NDPK2); FUNCTIONS IN: nucleoside diphosphate kinase activity, protein binding, ATP binding; INVOLVED IN: response to UV, auxin mediated signaling pathway, response to hydrogen peroxide, red, far-red light phototransduction; LOCATED IN: in 6 components; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Nucleoside diphosphate kinase, core (InterPro:IPR001564); BEST Arabidopsis thaliana protein match is: Nucleoside diphosphate kinase family protein (TAIR:AT4G09320.1); Has 9088 Blast hits to 8922 proteins in 2598 species: Archae - 291; Bacteria - 4641; Metazoa - 1094; Fungi - 170; Plants - 375; Viruses - 117; Other Eukaryotes - 2400 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR