F13347

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F13347

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • increased size (PATO:0000586)
    (Seed::Shape::large)
  • increased length (PATO:0000573)
    (Seed::Shape::long)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F13347

Determination of introduced cDNA(s)

  • RAFL06-78-H02 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL06-78-H02 - Resource: pda03728

    full reading [+] show sequence

    Nucleotide sequence: RAFL06-78-H02 - full reading 
    AAGATTCTTC ACATAGAGAA GTCACATGTT CCTTCTTCTT CTTCCTTCAT 
    CATCCCCAAA CACACACAAA CTAAAAAAAA GGCAAAACCC TAGATCCAAG 
    ATCAGACCTA ATCTAATCGA AACTCATAGC TGAAAAATGA AGAGAGATCA 
    TCACCAATTC CAAGGTCGAT TGTCCAACCA CGGGACTTCT TCTTCATCAT 
    CATCAATCTC TAAAGATAAG ATGATGATGG TGAAAAAAGA AGAAGACGGT 
    GGAGGTAACA TGGACGACGA GCTTCTCGCT GTTTTAGGTT ACAAAGTTAG 
    GTCATCGGAG ATGGCGGAGG TTGCTTTGAA ACTCGAACAA TTAGAGACGA 
    TGATGAGTAA TGTTCAAGAA GATGGTTTAT CTCATCTCGC GACGGATACT 
    GTTCATTATA ATCCGTCGGA GCTTTATTCT TGGCTTGATA ATATGCTCTC 
    TGAGCTTAAT CCTCCTCCTC TTCCGGCGAG TTCTAACGGT TTAGATCCGG 
    TTCTTCCTTC GCCGGAGATT TGTGGTTTTC CGGCTTCGGA TTATGACCTT 
    AAAGTCATTC CCGGAAACGC GATTTATCAG TTTCCGGCGA TTGATTCTTC 
    GTCTTCGTCG AATAATCAGA ACAAGCGTTT GAAATCATGC TCGAGTCCTG 
    ATTCTATGGT TACATCGACT TCAACGGGTA CGCAGATTGG TGGAGTCATA 
    GGAACGACGG TGACGACAAC CACCACGACA ACGACGGCGG CGGGTGAGTC 
    AACTCGTTCT GATATCCTGG TTGACTCGCA AGAGAACGGT GTTCGTTTAG 
    TCCACGCGCT TATGGCTTGT GCAGAAGCAA TCCAGCAGAA CAATTTGACT 
    CTAGCGGAAG CTCTTGTGAA GCAAATCGGA TGCTTAGCTG TGTCTCAAGC 
    CGGAGCTATG AGAAAAGTGG CTACTTACTT CGCCGAAGCT TTAGCGCGGC 
    GGATCTACCG TCTCTCTCCG CCGCAGAATC AGATCGATCA TTGTCTCTCC 
    GATACTCTTC AGATGCACTT TTACGAGACT TGTCCTTATC TTAAATTCGC 
    TCACTTCACG GCGAACCAAG CGATTCTCGA AGCTTTTGAA GGTAAGAAGA 
    GAGTACACGT CATTGATTTC TCGATGAACC AAGGTCTTCA ATGGCCTGCA 
    CTTATGCAAG CTCTTGCGCT TCGAGAAGGA GGTCCTCCAA CTTTCCGGTT 
    AACCGGAATT GGTCCACCGG CGCCGGATAA TTCTGATCAT CTTCATGAAG 
    TTGGTTGTAA ATTAGCTCAG CTTGCGGAGG CGATTCACGT AGAATTCGAA 
    TACCGTGGAT TCGTTGCTAA CAGCTTAGCC GATCTCGATG CTTCGATGCT 
    TGAGCTTAGA CCGAGCGATA CGGAAGCTGT TGCGGTGAAC TCTGTTTTTG 
    AGCTACATAA GCTCTTAGGT CGTCCCGGTG GGATAGAGAA AGTTCTCGGC 
    GTTGTGAAAC AGATTAAACC GGTGATTTTC ACGGTGGTTG AGCAAGAATC 
    GAACCATAAC GGACCGGTTT TCTTAGACCG GTTTACTGAA TCGTTACATT 
    ATTATTCGAC TCTGTTTGAT TCGTTGGAAG GAGTTCCGAA TAGTCAAGAC 
    AAAGTCATGT CTGAAGTTTA CTTAGGGAAA CAGATTTGTA ATCTGGTGGC 
    TTGTGAAGGT CCTGACAGAG TCGAGAGACA CGAAACGTTG AGTCAATGGG 
    GAAACCGGTT TGGTTCGTCC GGTTTAGCGC CGGCACATCT TGGGTCTAAC 
    GCGTTTAAGC AAGCGAGTAT GCTTTTGTCT GTGTTTAATA GTGGCCAAGG 
    TTATCGTGTG GAGGAGAGTA ATGGATGTTT GATGTTGGGT TGGCACACTC 
    GTCCACTCAT TACCACCTCC GCTTGGAAAC TCTCGACGGC GGCGTACTGA 
    GTTTGACTCG AAGCATACGA CGGTGGTGGA GTCGAGTCGA GTGAATTTGA 
    GATTGAGATC AGTGGACCGG TGATGACATA TGTTCGGACC AAGACCTAAA 
    CCGAACTGAA TCGAACCGTT TTGCCTTTTG TTTATTTTAT TTATTTTCGT 
    TCACTTGTTT AAAATTCTTA TATATATCGT TTTGGTAGGT CATTTTTAAT 
    TTATGCCTTT TTGGGATCAA TTTTTAATAG GCTGAGTTTG TATTTATTAA 
    TAAATTATCT TTATGAATTT TAAACTAAAA CTATGTTTTA ATCTCATTTA 
    AAAAAAAATT AATATT

    Gene models with high sequence identity

    1. AT2G01570.1 [+] show detail - GRAS family transcription factor family protein
      E-value: 0; Score: 99.91

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Transcription factor GRAS 5.6e-133
    HMMPfam Transcriptional factor DELLA, N-terminal 2.2e-35
    Coil coiled-coil 0
    ProfileScan Transcription factor GRAS 65.245

    See the detailed result >

    AT2G01570.1

    Model type
    Protein coding
    Short Description
    GRAS family transcription factor family protein
    Curator Summary
    Member of the VHIID/DELLA regulatory family. Contains homopolymeric serine and threonine residues, a putative nuclear localization signal, leucine heptad repeats, and an LXXLL motif. Putative transcriptional regulator repressing the gibberellin response and integration of phytohormone signalling. DELLAs repress cell proliferation and expansion that drives plant growth. The protein undergoes degradation in response to GA via the 26S proteasome. RGA1 binds to PIF3 and inhibits its DNA binding activity and thus affects the expression of PIF3 regulated genes. RGA may be involved in reducing ROS accumulation in response to stress by up-regulating the transcription of superoxide dismutases. Represses GA-induced vegetative growth and floral initiation. Rapidly degraded in response to GA. Involved in fruit and flower development.
    Computational Description
    REPRESSOR OF GA1-3 1 (RGA1); CONTAINS InterPro DOMAIN/s: Transcriptional factor DELLA, N-terminal (InterPro:IPR021914), Transcription factor GRAS (InterPro:IPR005202); BEST Arabidopsis thaliana protein match is: GRAS family transcription factor family protein (TAIR:AT1G14920.1); Has 2724 Blast hits to 2666 proteins in 317 species: Archae - 0; Bacteria - 6; Metazoa - 0; Fungi - 4; Plants - 2709; Viruses - 0; Other Eukaryotes - 5 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR