F13523

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Phenotypes

Morphological phenotypes

Generation	Anatomical entity /structure development stage	Phenotypes
T1
	rosette leaf (PO:0000014)	upturned (PATO:0002031) (Rosette leaf::Shape::hyponastic) abnormal (PATO:0000460) (Rosette leaf::Others) increased width (PATO:0000600) (Rosette leaf::Shape::wide)
	stem (PO:0009047)	abnormal (PATO:0000460) (Stem::Others) present in fewer numbers in organism (PATO:0001997) (Stem::Number::few)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F13523

Determination of introduced cDNA(s)

RAFL09-93-L22 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL09-93-L22 - Resource: pda17911

full reading [+] show sequence

GAGAAATCGC TTCACCATTC CCAAATCTCT CTTCCATTTT CTCCACACAA 
ATTTCTCTTC AATCTCCGAT AATGGAAGGA ACCGGCGTTG TGGCGGTGTA 
CGGTAACGGT GCGATAACGG AGGCGAAGAA ATCTCCCTTC TCCGTGAAGG 
TCGGTTTGGC TCAGATGCTC CGTGGTGGTG TTATCATGGA TGTCGTCAAC 
GCCGAGCAAG CTCGTATCGC CGAGGAGGCT GGTGCTTGCG CCGTCATGGC 
TTTGGAGCGT GTTCCTGCTG ATATCCGCGC TCAAGGAGGC GTCGCTCGTA 
TGAGCGATCC ACAAATGATT AAAGAAATCA AACAAGCCGT TACGATTCCG 
GTGATGGCTA AGGCTAGGAT TGGTCATTTC GTTGAAGCTC AGATCCTTGA 
AGCAATTGGA ATCGATTACA TCGATGAGAG CGAGGTTTTG ACTCTTGCTG 
ATGAAGATCA TCACATCAAC AAGCATAATT TCCGGATCCC GTTCGTTTGC 
GGTTGCCGGA ATCTCGGCGA GGCTCTGAGG AGGATCCGTG AAGGTGCGGC 
GATGATTAGG ACCAAAGGTG AAGCTGGAAC CGGTAACATT ATTGAAGCTG 
TGAGGCATGT GAGGTCTGTT AATGGTGACA TTAGGGTTTT GCGAAACATG 
GATGATGATG AGGTTTTCAC TTTCGCTAAG AAATTAGCCG CTCCGTACGA 
TCTCGTGATG CAGACTAAGC AGCTTGGTCG TCTTCCTGTA GTCCAATTCG 
CCGCCGGTGG AGTGGCTACT CCGGCTGATG CAGCTCTCAT GATGCAGCTT 
GGATGTGATG GTGTCTTTGT TGGTTCTGGT ATCTTCAAGA GCGGTGACCC 
AGCTCGTCGT GCACGTGCCA TTGTTCAGGC TGTGACTCAT TACAGTGACC 
CTGAGATGCT TGTGGAGGTG AGCTGTGGGC TTGGAGAAGC CATGGTTGGG 
ATCAATCTCA ACGATGAGAA GGTTGAGAGG TTCGCTAATC GCTCCGAGTG 
ATCAAAGAAA TAAAAGGTAA AATATCTCAG ACGAAATGGT TTCAGAATTT 
TCTCAGACCA TTTTGCAGTA ATCTCTTTGA AAAGAAGAAG ATGATGATAT 
TGTTGGTAGT TTGTATCCTT TGTGTTTTCC TTATAATCTT TGATAGTCTT 
TTGTTATTGT AACTCGTAAT CCCTTTGCAA GAACAAGTTT GTCAGTTATA 
ATAATGTACT ACTCTCTTGA TCGATCAGTT GGTTTTGAAT CTGATATATT 
CTTCGATCCA AAAAAAAAA

Gene models with high sequence identity

AT5G01410.1 [+] show detail - Aldolase-type TIM barrel family protein
E-value: 0; Score: 100.00

InterPro Scan Digest

Program	Description	E-value
superfamily	Ribulose-phosphate binding barrel	5.3e-49
PatternScan	Vitamin B6 biosynthesis protein	0
HMMTigr	Vitamin B6 biosynthesis protein	1.1e-165
ProfileScan	Vitamin B6 biosynthesis protein	174.634
HAMAP	Vitamin B6 biosynthesis protein	50.496
HMMPfam	Vitamin B6 biosynthesis protein	8.9e-116
HMMPIR	Vitamin B6 biosynthesis protein	7.1e-216

See the detailed result >

AT5G01410.1

Model type: Protein coding
Short Description: Aldolase-type TIM barrel family protein
Curator Summary: Encodes a protein predicted to function in tandem with PDX2 to form glutamine amidotransferase complex with involved in vitamin B6 biosynthesis.
Computational Description: REDUCED SUGAR RESPONSE 4 (RSR4); FUNCTIONS IN: protein homodimerization activity, protein heterodimerization activity; INVOLVED IN: in 12 processes; LOCATED IN: cytosol, endomembrane system, plasma membrane; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Vitamin B6 biosynthesis protein (InterPro:IPR001852), Ribulose-phosphate binding barrel (InterPro:IPR011060); BEST Arabidopsis thaliana protein match is: pyridoxine biosynthesis 1.1 (TAIR:AT2G38230.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink).
Link: InterPro Scan - TAIR