F14207

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F14207

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
cauline leaf (PO:0000013)
  • desaturated green (PATO:0001272)
    (Cauline leaf::Color::pale)
stem (PO:0009047)
  • increased thickness (PATO:0000591)
    (Stem::Shape::thick)
  • desaturated green (PATO:0001272)
    (Stem::Color::pale)
  • abnormal (PATO:0000460)
    (Stem::Others)
  • abnormal (PATO:0000460)
    (Stem::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F14207

Determination of introduced cDNA(s)

  • RAFL09-21-G21 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL09-21-G21 - Resource: pda08023

    full reading [+] show sequence

    Nucleotide sequence: RAFL09-21-G21 - full reading 
    AAAAACTTGA GAAGATGATG GCTGATGACA CTGAACTCGT CAGTAACACT 
    GACCAGAAGA TGGAAGACGC TTCAGCCGAA AACCCTGCCC GAGCTGATCC 
    CCCCTCATCG GATGATTCCG GCGATTCTGA TTCCGACTCA GAAGATGAAG 
    CCGAGTCGAA TCACCAGATT GTAACCTTGG AGTCGGAGCT CTCCGCTAAT 
    CCTTACAACT ACGATGCTTA TGTCCAATAC ATTAAGCTTC TCAGGAAAAC 
    AGCCAACCTT GAAAAGCTAA GACAAGCAAG GGAAGCTATG AGTGCCATAT 
    TTCCGTTGAG TCCTTCCTTG TGGCTTGAAT GGGCAAGGGA TGAAGCGTCT 
    TTAGCCGCTA GTGAGAATGT GCCTGAAATT GTGATGCTTT ATGAACGGGG 
    ACTTTCTGAT TATCAGTCTG TATCTCTTTG GTGTGACTAC CTGAGCTTTA 
    TGCTAGAATT TGATCCATCA GTGCGTGGAT ACCCATCTGA AGGTATTTCC 
    AAAATGCGGA GTCTTTTTGA ACGTGCTATT CCGGCTGCGG GCTTCCATGT 
    TACTGAAGGC AATAGAATAT GGGAAGGATA TAGAGAATTT GAGCAAGGTG 
    TCTTAGCCAC TATCGACGAG GCTGACATTG AGGAGAGAAA TAAGCAGATA 
    CAAAGGATCA GAAGCATATT CCACCGTCAC CTGTCTGTTC CTCTAGAAAA 
    CTTGAGCTCC ACCCTTATTG CTTACAAGAC TTGGGAGTTG GAACAGGGCA 
    TTGACCTTGA TATTGGATCT GATGATTTGA GTAAGGTTTC TCATCAGGTT 
    GCTGTTGCAA ACAAGAAGGC ACAGCAGATG TATAGTGAAC GTGCCCATCT 
    TGAAGAAAAC ATATCTAAGC AAGATTTATC TGACACGGAA AAGTTTCAGG 
    AATTCATGAA TTATATAAAG TTTGAGAAGA CTTCTGGAGA TCCTACCCGA 
    GTTCAAGCTA TATATGAACG TGCTGTGGCT GAATACCCGG TTTCAAGTGA 
    CTTGTGGATA GACTACACCG TGTATCTTGA TAAAACTTTG AAGGTTGGAA 
    AAGCTATAAC GCATGCCTAT TCTAGGGCGA CTAGAAGTTG TCCATGGACT 
    GGAGATCTTT GGGCACGTTA TTTACTTGCT TTAGAGCGTG GTTCTGCATC 
    CGAGAAAGAG ATTTATGATG TCTTTGAGAA GTCATTGCAG TGCACATTTT 
    CCTCCTTTGA AGAGTACCTG GATTTATACC TCACTCGAGT AGATGGCCTG 
    AGAAGGAGAA TGTTATCAAC TAGGATGCTA GAGGCTTTGG ACTATTCTCT 
    GATAAGAGAA ACTTTTCAGC AAGCATCAGA TTACCTAACG CCTCACATGC 
    AAAATACAGA CAGTTTGCTA CATTTGCATA CTTATTGGGC TAACTTGGAA 
    CTTAATATTG GAAAAGATCT AGCTGGCGCA CGAGGTGTTT GGGATAGCTT 
    TCTGAAGAAG AGTGGTGGGA TGCTAGCAGC CTGGCATGCA TACATTGATA 
    TGGAAGTGCA TTTGGGGCAT ATAAAGGAAG CTAGGTCTAT TTACCGGAGG 
    TGCTATACAA GAAAGTTTGA TGGAACTGGC TCAGAAGACA TATGCAAAGG 
    ATGGTTGCGC TTTGAGAGGG AGCATGGGGA TTTGGAACAT TTTGACCTAG 
    CAGTTCAAAA GGTTATGCCA CGCTTGGAAG AGCTACAATT GATGAGGTTA 
    CAACAAGAGT CTACACCAGT CAAACCGTCA GCTGGTCTGA AAGAGCATAG 
    CTCACAAAAG AGAAAAGCAG AACAGAATGT AGAAGAAGAG TCTTTGGCTA 
    AGCGGCAGAA GAGGAAAAGT CAAAAAGAAG TGGACCTAGG TGGTCAGAGT 
    GCAACTGTAC CAGCAACAAA AAACGTTAAA GCAGAGAATG GAAAAACAGC 
    TGATTCAGAT AAAGAAGAGA CAGAAGATGT CAAACCACTG AAGCCGAAAG 
    TTTATAGAGA CGAGTGTACA GCTTTTATTT CGAATCTCAG TGTCAAGGCA 
    CAAGAGGAAG ATATACGTAA ATTCTTTGGT GATGATGGTG GGGTTGATTC 
    CATTCGCATT CTCCATCACA AAGACACTGG AAAACCAAGG GGACTGGCAT 
    ATGCTGATTT TGTGGATGAT GAACACCTTG CAGCAGCTAT AGCAAAGAAC 
    AGAAAGATGT TTTTTGGTAA GAAGATCAGC ATAGCACGGT CAAATCCAAA 
    GAAGGGTAAA AAAGAATTCA CTCGGCGTGG AAATGATGGT TCTGGGAATT 
    CAAAGGACCC TTCCCTGATT TCTGAGAAGG CGAAAGCACC ACTTGGTGGA 
    GAAACTGAGG GTGAAAGGAA AGGAAATGAA GTGGAAGTGA GGGGTAAGAA 
    CACGTTTGCT GTCCCAAGAA ATGTTAAACC ACTCGGCTAC ACGACACCTA 
    AACCCAGCGC AGATGAGACA CCAAAATCAA ATGATGAGTT CAGGAATATG 
    TTCCTTAAAA AGTGAGACTT TTGCTTGGTC CCTATCTTCC TTCCCTTAGA 
    TACTTGTCTT GTTCTACTTT TCGGAAGTAA TCTTATGTTG TAGACCAGCA 
    ACATAGGATC TGCTACTCTC TTTTGTCAAG ATAACTAGTT GAGCTTCTAT 
    ATAACTAATA TATAAATTTA AGTCTTGC

    Gene models with high sequence identity

    1. AT4G24270.1 [+] show detail - EMBRYO DEFECTIVE 140
      E-value: 0; Score: 99.89

     

    InterPro Scan Digest

    Program Description E-value
    ProfileScan RNA recognition motif, RNP-1 13.891
    superfamily RNA-binding domain, RBD 8.5e-19
    superfamily TPR-like 5.3e-09
    Gene3D Nucleotide-binding, alpha-beta plait 9.5e-17
    Coil coiled-coil 0
    Coil coiled-coil 0
    HMMSmart RNA-processing protein, HAT helix 140
    HMMSmart RNA-processing protein, HAT helix 12
    HMMSmart RNA-processing protein, HAT helix 12
    HMMSmart RNA-processing protein, HAT helix 230
    HMMSmart RNA-processing protein, HAT helix 0.0005
    HMMSmart RNA-processing protein, HAT helix 47
    HMMSmart RNA-processing protein, HAT helix 43
    HMMSmart RNA-processing protein, HAT helix 26
    HMMSmart RNA recognition motif, RNP-1 4.6e-16
    HMMPfam RNA recognition motif, RNP-1 1.2e-11
    HMMPfam Lsm interaction 2.5e-08
    HMMPanther RIBONUCLEOPROTEIN-RELATED 6.3e-188

    See the detailed result >

    AT4G24270.1

    Model type
    Protein coding
    Short Description
    EMBRYO DEFECTIVE 140
    Curator Summary
    A locus involved in embryogenesis. Mutations in this locus result in embryo lethality.
    Computational Description
    EMBRYO DEFECTIVE 140 (EMB140); FUNCTIONS IN: RNA binding, nucleotide binding, nucleic acid binding; INVOLVED IN: embryo development ending in seed dormancy; LOCATED IN: intracellular; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Lsm interaction (InterPro:IPR008669), RNA-processing protein, HAT helix (InterPro:IPR003107), RNA recognition motif, RNP-1 (InterPro:IPR000504), Nucleotide-binding, alpha-beta plait (InterPro:IPR012677); BEST Arabidopsis thaliana protein match is: crooked neck protein, putative / cell cycle protein, putative (TAIR:AT5G45990.1); Has 6865 Blast hits to 6296 proteins in 569 species: Archae - 0; Bacteria - 876; Metazoa - 2538; Fungi - 1681; Plants - 1001; Viruses - 0; Other Eukaryotes - 769 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR