F14634

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F14634

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
cauline leaf (PO:0000013)
  • desaturated green (PATO:0001272)
    (Cauline leaf::Color::pale)
rosette leaf (PO:0000014)
  • desaturated green (PATO:0001272)
    (Rosette leaf::Color::pale)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
fruit (PO:0009001)
  • abnormal (PATO:0000460)
    (Silique::Others)
stem (PO:0009047)
  • present in fewer numbers in organism (PATO:0001997)
    (Stem::Number::few)
T2  
rosette leaf (PO:0000014)
  • desaturated green (PATO:0001272)
    (Rosette leaf::Color::pale)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
fruit (PO:0009001)
  • abnormal (PATO:0000460)
    (Silique::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F14634

Determination of introduced cDNA(s)

  • RAFL04-19-K24 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL04-19-K24 - Resource: pda00556

    full reading [+] show sequence

    Nucleotide sequence: RAFL04-19-K24 - full reading 
    ACTTATCTCC GGTAACGGAC AACCACTCGT TTCCCGAAAC AGCAACTCAC 
    AGACTCACAC CACTCCAGTC TCCGGCTTAA CTACCACCAG AGACGATTCT 
    CTCTTCCGTC GGTTCTATGA CTTCGATTCT CAACACTGTC TCCACCATCC 
    ACTCTTCCAG AGTTACCTCC GTCGATCGAG TCGGAGTCCT CTCTCTTCGG 
    AATTCGGATT CCGTTGAGTT CACTCGCCGG CGTTCTGGTT TCTCGACGTT 
    GATCTACGAA TCACCCGGGC GGAGATTTGT TGTGCGTGCG GCGGAGACTG 
    ATACTGATAA AGTTAAATCT CAGACACCTG ACAAGGCACC AGCCGGTGGT 
    TCAAGCATTA ACCAGCTTCT CGGTATCAAA GGAGCATCTC AAGAAACTAA 
    TAAATGGAAG ATTCGTCTTC AGCTTACAAA ACCAGTCACT TGGCCTCCAC 
    TGGTTTGGGG AGTCGTCTGT GGTGCTGCTG CTTCAGGGAA CTTTCATTGG 
    ACCCCAGAGG ATGTTGCTAA GTCGATTCTT TGCATGATGA TGTCTGGTCC 
    TTGTCTTACT GGCTATACAC AGACAATCAA CGACTGGTAT GATAGAGATA 
    TCGACGCAAT TAATGAGCCA TATCGTCCAA TTCCATCTGG AGCAATATCA 
    GAGCCAGAGG TTATTACACA AGTCTGGGTG CTATTATTGG GAGGTCTTGG 
    TATTGCTGGA ATATTAGATG TGTGGGCAGG GCATACCACT CCCACTGTCT 
    TCTATCTTGC TTTGGGAGGA TCATTGCTAT CTTATATATA CTCTGCTCCA 
    CCTCTTAAGC TAAAACAAAA TGGATGGGTT GGAAATTTTG CACTTGGAGC 
    AAGCTATATT AGTTTGCCAT GGTGGGCTGG CCAAGCATTG TTTGGCACTC 
    TTACGCCAGA TGTTGTTGTT CTAACACTCT TGTACAGCAT AGCTGGGTTA 
    GGAATAGCCA TTGTTAACGA CTTCAAAAGT GTTGAAGGAG ATAGAGCATT 
    AGGACTTCAG TCTCTCCCAG TAGCTTTTGG CACCGAAACT GCAAAATGGA 
    TATGCGTTGG TGCTATAGAC ATTACTCAGC TTTCTGTTGC CGGATATCTA 
    TTAGCATCTG GGAAACCTTA TTATGCGTTG GCGTTGGTTG CTTTGATCAT 
    TCCTCAGATT GTGTTCCAGT TTAAATACTT TCTCAAGGAC CCTGTCAAAT 
    ACGACGTCAA GTACCAGGCA AGCGCGCAGC CATTCTTGGT GCTCGGAATA 
    TTTGTAACGG CATTAGCATC GCAACACTGA AAAAGGCGTA TTTTGATGGG 
    GTTTTGTCGA AAGCAGAGGT GTTGACACAT CAAATGTGGG CAAGTGATGG 
    CATCAACTAG TTTAAAAGAT TTTGTAAAAT GTATGTACCG TTATTACTAG 
    AAACAACTCC TGTTGTATCA ATTTAGCAAA ACGGCTGAGA AATTGTAATT 
    GATGTTAC

    Gene models with high sequence identity

    1. AT3G51820.1 [+] show detail - UbiA prenyltransferase family protein
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam UbiA prenyltransferase 5.2e-42
    HMMTigr Chlorophyll synthase, ChlG 2.5e-251
    HMMTigr Bacteriochlorophyll/chlorophyll synthetase 1.5e-150
    HMMPanther BACTERIOCHLOROPHYLL SYNTHASE 1.4e-80
    HMMPanther PRENYLTRANSFERASES 1.4e-80

    See the detailed result >

    AT3G51820.1

    Model type
    Protein coding
    Short Description
    UbiA prenyltransferase family protein
    Curator Summary
    Encodes a protein with chlorophyll synthase activity. This enzyme has been shown to perform the esterification of chlorophyllide (a and b), the last step of chlorophyll biosynthesis. Although it can use either geranylgeranyl pyrophosphate (GGPP) or phytyl pyrophosphate (PhyPP) as substrates, the esterification reaction was faster with GGPP than with PhyPP.
    Computational Description
    G4; FUNCTIONS IN: chlorophyll synthetase activity; INVOLVED IN: chlorophyll biosynthetic process; LOCATED IN: chloroplast thylakoid membrane, chloroplast; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Bacteriochlorophyll/chlorophyll synthetase (InterPro:IPR006372), Chlorophyll synthase, ChlG (InterPro:IPR011799), UbiA prenyltransferase (InterPro:IPR000537); BEST Arabidopsis thaliana protein match is: homogentisate prenyltransferase (TAIR:AT3G11945.1); Has 2782 Blast hits to 2782 proteins in 800 species: Archae - 310; Bacteria - 1584; Metazoa - 63; Fungi - 24; Plants - 236; Viruses - 0; Other Eukaryotes - 565 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR