F15327

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F15327

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F15327

Determination of introduced cDNA(s)

  • RAFL11-12-F09 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL11-12-F09 - Resource: pdx27069

    5 prime EST [+] show sequence

    Nucleotide sequence: RAFL11-12-F09 - 5 prime EST 
    GGAGAGCTAA ATCATTTACG TTTTGATCGA CAAAAATGGA GTTGATAAAC 
    GACTTTCTCA ACCTAACTGC TCCTTTCTTC ACCTTCTTTG GTCTCTGCTT 
    CTTCTTGCCT CCTTTTTACT TCTTCAAGTT CTTGCAGTCT ATTTTCTCGA 
    CAATTTTCTC TGAAAATCTT TACGGGAAAG TTGTTCTCAT CACTGGTGCT 
    TCCTCCGGTA TAGGCGAGCA ATTGGCATAT GAGTACGCAT GTAGAGGTGC 
    ATGTTTAGCC CTGACCGCCC GAAGGAAGAA CCGTCTAGAG GAAGTGGCAG 
    AGATTGCTCG TGAACTCGGA TCTCCCAATG TTGTTACCGT TCATGCTGAT 
    GTCTCCAAAC CTGATGACTG TAGACGAATC GTTGATGACA CCATCACCCA 
    TTTTGGCAGA TTGGATCATC TTGTAAATAA TGCTGGGATG ACGCAGATTT 
    CAATGTTTGA GAACATTGAA GATATAACGA GGACAAAAGC AGTTTTGGAT 
    ACTAACTTCT GGGGATCGGT TNATACCACT CGTGCTGCGC TTNCATACCN 
    TTAGACAAAG TAATGGTAAG ATTGTGGCTA TGTC

    Gene models with high sequence identity

    1. AT5G50700.1 [+] show detail - hydroxysteroid dehydrogenase 1
      E-value: 0; Score: 99.49

    3 prime EST [+] show sequence

    Nucleotide sequence: RAFL11-12-F09 - 3 prime EST 
    AAAGACACAT AGTTAATATA CATAGTCAAA AGTACCATTA ACAAAAACAA 
    ACCTACATTT TCACCCATTT CTTTGATAAT TATTACTCAT TGCACATACT 
    CATGATACAT CTCATCCATT TATTATGTAT TGACCCCTCT CTAATCCGAC 
    TTGATTTCTG GAGTTCTGAT AGATTCCGGG TACAGAGTAC TACGTACACC 
    AGGAATGTCC ATGATCCTCT TGTTGAGTGG AGTATCCTCG GACATACCAG 
    TTCCAGTCAT GTTCAGTAAC CGACAACCCC ACTCAATCAA CTCCGGAAAT 
    AGCACTTTCC AAAGGTACGT CACCTTAAAC CATGATGGCT CTGTCAAGTA 
    TTTTTGTTTC CGGGCACACA CCGTTCACTA TGGACTTGGC ACATCCTGAT 
    CCTGACGCTG

    Gene models with high sequence identity

    1. AT5G50700.1 [+] show detail - hydroxysteroid dehydrogenase 1
      E-value: 0; Score: 98.04

    AT5G50700.1

    Model type
    Protein coding
    Short Description
    hydroxysteroid dehydrogenase 1
    Curator Summary
    Encodes a hydroxysteroid dehydrogenase HSD1. Two copies of HSD1 (At5g50600 and At5g50700) exist in the Arabidopsis genome as a result of an exact 33-kb duplication on chromosome 5 encompassing seven genes. There are five homologs of HSD1 in Arabidopsis (HSD2-At3g47350, HSD3-At3g47360, HSD4-At5g50590, HSD4-At5g50690 and HSD6-At5g50770; HSD4 has two copies due to the same gene duplication event occurred to HSD1) (Plant Cell Physiology 50:1463). At5g50690 is also named as HSD7 (Plant Physiology 145:87). HSD1 is identified from the proteome of oil bodies from mature seeds. Transcription of HSD1 is specifically and highly induced in oil-accumulating tissues of mature seeds; transcript disappears during germination.
    Computational Description
    hydroxysteroid dehydrogenase 1 (HSD1); FUNCTIONS IN: oxidoreductase activity, binding, catalytic activity; INVOLVED IN: oxidation reduction, metabolic process; LOCATED IN: endomembrane system; EXPRESSED IN: seed; CONTAINS InterPro DOMAIN/s: Short-chain dehydrogenase/reductase, conserved site (InterPro:IPR020904), NAD(P)-binding domain (InterPro:IPR016040), Glucose/ribitol dehydrogenase (InterPro:IPR002347), Short-chain dehydrogenase/reductase SDR (InterPro:IPR002198); BEST Arabidopsis thaliana protein match is: hydroxysteroid dehydrogenase 1 (TAIR:AT5G50600.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR