F15845

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F15845

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1  
stem (PO:0009047)
  • abnormal (PATO:0000460)
    (Stem::Others)
  • abnormal (PATO:0000460)
    (Stem::Others)
stem internode (PO:0020142)
  • decreased length (PATO:0000574)
    (Stem::Shape::short internode)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F15845

Determination of introduced cDNA(s)

  • RAFL04-17-O24 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL04-17-O24 - Resource: pda00651

    full reading [+] show sequence

    Nucleotide sequence: RAFL04-17-O24 - full reading
    ATTTGCTCAC CTTTCGCCGA TTGATTTTGT ACATCACACG CAGACGCAGC 
    TTTTAGTATT TTTTTCAACA ATCGCTTTTT TTTTTTTCTC GATCTCCGTG
    AAGCTCTCGA ATCTCTGGTT TTGAGTTTTG GAGAATGGTG TTTTTCCGCA
    GCGTATCGGC CTTTACTAGG CTGAGGTCTC GCGTCGGCCA ACAATCTTCA
    CTCAGCAATT CTGTCAGATG GATTCAGATG CAGAGCTCTA CCGACCTGGA
    CCTGAAGTCG CAGCTGCAAG AGTTAATTCC GGAACAGCAG GACCGTCTGA
    AGAAACTGAA GTCAGAACAT GGGAAGGTCC AACTGGGAAA CATCACTGTT
    GATATGGTTA TTGGTGGAAT GAGAGGGATG ACTGGATTGC TTTGGGAAAC
    CTCATTGCTT GACCCGGAAG AGGGTATTCG CTTTAGGGGA TTGTCGATTC
    CTGAGTGCCA GAAAGTATTA CCTACTGCCC AGTCTGGAGC TGAACCATTA
    CCGGAGGGTC TTTTGTGGCT TCTCTTAACT GGAAAGGTAC CTAGCAAAGA
    GCAAGTTGAA GCACTGTCGA AAGACTTGGC GAACCGTGCT GCTGTGCCAG
    ATTATGTGTA CAATGCCATC GATGCTCTGC CTTCCACAGC TCATCCAATG
    ACTCAATTTG CTAGCGGTGT TATGGCCCTC CAGGTGCAAA GTGAGTTTCA
    AAAGGCATAT GAGAATGGAA TTCATAAGTC AAAGTTCTGG GAGCCAACAT
    ATGAGGATTG CCTCAACCTG ATTGCTCGTG TTCCTGTTGT AGCTGCATAT
    GTTTATCGAA GGATGTATAA GAATGGTGAT TCCATTCCCT CAGATAAATC
    TTTGGATTAT GGTGCAAATT TTTCCCACAT GTTGGGATTT GATGATGAAA
    AGGTGAAAGA GCTCATGAGG CTTTACATCA CCATCCACAG TGATCATGAA
    GGTGGAAATG TTAGTGCTCA CACTGGTCAC CTGGTTGGTA GTGCACTTTC
    AGACCCATAT CTGTCATTTG CAGCTGCATT AAATGGTTTA GCTGGGCCAC
    TCCATGGTTT GGCTAATCAG GAAGTTTTGC TTTGGATCAA ATCAGTCGTA
    GAGGAATGTG GAGAAGATAT ATCAAAAGAA CAGTTGAAAG AATATGTTTG
    GAAAACATTA AACAGTGGCA AGGTTATTCC GGGATATGGT CACGGTGTTC
    TGCGCAATAC TGATCCAAGA TATGTATGCC AAAGAGAATT TGCCTTGAAG
    CATCTACCTG ACGACCCTCT TTTCCAGCTG GTGTCAAAGC TTTATGAAGT
    TGTGCCTCCT GTTCTCACTG AGCTTGGAAA GGTGAAGAAC CCGTGGCCAA
    ATGTTGATGC TCACAGTGGG GTCTTGCTGA ACCACTATGG TCTAACCGAA
    GCAAGGTACT ACACCGTGCT CTTTGGTGTT TCAAGGAGTC TTGGCATCTG
    CTCTCAGCTT ATATGGGACC GAGCTCTTGG ACTTGCACTT GAGAGGCCAA
    AGAGTGTTAC CATGGACTGG CTTGAAGCCC ATTGTAAGAA AGCTTCATCT
    GCTTAAGTCA CCACCGAGTC CTGAGTTGTC GCACTCTCTC GTGTTTCAAC
    AAAAGTCATT CTCTTCCACA CTGGATAGCT TTTTTTGTTT TTACCTTCTA
    TTGACAGAAG AAACATTTGT TCATGTAGAA ATTGTTCCTG AGAAACACAT
    GCTTGGTGCA AATTTGCATT TCCGGGAAAC TCGTGATAAT AAATAAACAG
    TTGTATT

    Gene models with high sequence identity

    1. AT2G44350.1 [+] show detail - Citrate synthase family protein
      E-value: 0; Score: 99.89

     

    InterPro Scan Digest

    Program Description E-value
    HMMTigr Citrate synthase, eukaryotic 0
    superfamily Citrate synthase-like, core 2e-145
    Gene3D Citrate synthase-like, large alpha subdomain 9.1e-101
    PatternScan Citrate synthase active site 0
    HMMPanther Citrate synthase-like 5.4e-217
    FPrintScan Citrate synthase-like 1.2e-53
    FPrintScan Citrate synthase-like 1.2e-53
    FPrintScan Citrate synthase-like 1.2e-53
    FPrintScan Citrate synthase-like 1.2e-53
    FPrintScan Citrate synthase-like 1.2e-53
    FPrintScan Citrate synthase-like 1.2e-53
    HMMPfam Citrate synthase-like 1.2e-106
    Coil coiled-coil 0

    See the detailed result >

    AT2G44350.1

    Model type
    Protein coding
    Short Description
    Citrate synthase family protein
    Curator Summary
    encodes a mitochrondrion targeted citrate synthase, the first enzyme of the tricarboxylic acid cycle, catalyzing the condensation of acetyl-CoA and oxaloacetate, finally yielding citrate and CoA.
    Computational Description
    ATCS; FUNCTIONS IN: citrate (SI)-synthase activity, zinc ion binding, ATP binding; INVOLVED IN: response to cadmium ion, tricarboxylic acid cycle; LOCATED IN: mitochondrion, cell wall, chloroplast; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Citrate synthase-like, large alpha subdomain (InterPro:IPR016142), Citrate synthase, eukaryotic (InterPro:IPR010109), Citrate synthase active site (InterPro:IPR019810), Citrate synthase-like (InterPro:IPR002020), Citrate synthase-like, core (InterPro:IPR016141); BEST Arabidopsis thaliana protein match is: citrate synthase 5 (TAIR:AT3G60100.1); Has 10534 Blast hits to 10528 proteins in 2768 species: Archae - 158; Bacteria - 6911; Metazoa - 258; Fungi - 320; Plants - 178; Viruses - 0; Other Eukaryotes - 2709 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR