F16029

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F16029

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
cauline leaf (PO:0000013)
  • decreased size (PATO:0000587)
    (Cauline leaf::Shape::small)
rosette leaf (PO:0000014)
  • decreased size (PATO:0000587)
    (Rosette leaf::Shape::small)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
  • decreased width (PATO:0000599)
    (Rosette leaf::Shape::narrow)
stem (PO:0009047)
  • abnormal (PATO:0000460)
    (Stem::Others)
stem internode (PO:0020142)
  • decreased length (PATO:0000574)
    (Stem::Shape::short internode)
T2  
stem (PO:0009047)
  • decreased thickness (PATO:0000592)
    (Stem::Shape::thin)
  • abnormal (PATO:0000460)
    (Stem::Others)
stem internode (PO:0020142)
  • decreased length (PATO:0000574)
    (Stem::Shape::short internode)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F16029

Determination of introduced cDNA(s)

  • RAFL16-35-L09 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL16-35-L09 - Resource: pdz88576

    5 prime EST [+] show sequence

    Nucleotide sequence: RAFL16-35-L09 - 5 prime EST 
    AACCGGCTGT CTGGAGAGTT TTTTTTTTTT TTATTTTTTT TTTTTTTTTT 
    TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTAAAAAA 
    AAAAAANNAA

    Gene models with high sequence identity

    Not founds.

    3 prime EST [+] show sequence

    Nucleotide sequence: RAFL16-35-L09 - 3 prime EST 
    CAATGCCCAT CATATTGTAT ACAACTCTTC TAAATAAACA AAAGAGTTGT 
    CTTCTAAATA ATCATCTGAC AGCTATCTTT GCAGTTCTGT GTAGATTCAA 
    CCATAAATTT AAAACCAAAT TTCGATCTTT GCAATAATAA TTCACATGCT 
    AAGAAACAGG CAATTGCATT ATCAAAAATT CGA

    Gene models with high sequence identity

    1. AT5G02820.1 [+] show detail - Spo11/DNA topoisomerase VI, subunit A protein
      E-value: 7e-93; Score: 98.91

    AT5G02820.1

    Model type
    Protein coding
    Short Description
    Spo11/DNA topoisomerase VI, subunit A protein
    Curator Summary
    Involved in the patterning and shape of leaf trichomes. Encodes the DNA topoisomerase VI SPO11-3, involved in endoreduplication
    Computational Description
    ROOT HAIRLESS 2 (RHL2); CONTAINS InterPro DOMAIN/s: Spo11/DNA topoisomerase VI, subunit A, N-terminal (InterPro:IPR013049), DNA topoisomerase VI, subunit A (InterPro:IPR004085), Spo11/DNA topoisomerase VI, subunit A (InterPro:IPR002815); BEST Arabidopsis thaliana protein match is: Spo11/DNA topoisomerase VI, subunit A protein (TAIR:AT3G13170.1); Has 936 Blast hits to 932 proteins in 314 species: Archae - 223; Bacteria - 16; Metazoa - 170; Fungi - 119; Plants - 152; Viruses - 0; Other Eukaryotes - 256 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR