F16436

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F16436

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F16436

Determination of introduced cDNA(s)

  • RAFL06-88-G20 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL06-88-G20 - Resource: pda07773

    full reading [+] show sequence

    Nucleotide sequence: RAFL06-88-G20 - full reading 
    TGAATCAGTT TCGTGTGTTT GGAGAAGAAG AAGAACAGAT CAAATACGAG 
    GAGAGATCTC TAAAGAGATT TATCGTTTCA AATTTGTTCA CGTTGAGAAG 
    TTTAATTTAG ATTAAACAAC AAAAAGATGG GTTACTGGAA TTCCAAGGTT 
    GTTCCAAAAT TCAAGAAGTT ATTCGAGAAA AATAGTGCTA AGAAGGCTGC 
    TGCTGCTGAA GCTACCAAGA CCTTTGATGA ATCTAAGGAA ACAATCAACA 
    AGGAAATTGA GGAGAAAAAG ACAGAACTCC AACCAAAGGT CGTGGAAACC 
    TATGAAGCCA CGTCTGCAGA AGTCAAGGCT TTGGTGAGAG ACCCTAAGGT 
    GGCTGGTTTG AAGAAAAACT CAGCGGCTGT GCAGAAGTAC CTCGAGGAGC 
    TAGTCAAAAT TGAATTCCCC GGATCAAAAG CGGTGAGTGA GGCTTCGTCT 
    AGCTTCGGAG CTGGCTATGT CGCAGGACCG GTCACGTTCA TATTCGAGAA 
    GGTATCTGTT TTCCTCCCGG AGGAGGTGAA GACTAAAGAA ATACCGGTGG 
    AGGAAGTGAA AGCTGAAGAA CCTGCCAAAA CTGAAGAACC AGCCAAAACC 
    GAAGGAACAA GTGGTGAGAA AGAGGAGATT GTTGAAGAGA CAAAGAAAGG 
    CGAGACCCCT GAAACCGCGG TCGTGGAGGA GAAGAAACCA GAGGTAGAGG 
    AGAAGAAGGA AGAGGCTACT CCGGCTCCGG CAGTGGTTGA AACTCCAGTT 
    AAGGAACCGG AGACAACGAC GACAGCGCCA GTGGCTGAAC CACCAAAGCC 
    TTGATTTGTT TTCAAGATGG TATGATTACT TCTCTTGTAT GAAAACATCT 
    TTGTACGTAA CAAAAAAATG AAAGGAAGAA ACGGAAAGGA ACAAAAAAAA 
    AAAACTTATT TTTCATTCTT TTTTTCAATT TGGTTTGTCT TTGTGTGGAT 
    AAATCTCTCT GTTTTTTTTT TAATTATTGG GAAAGTCTTT AATGTGTTAT 
    TTATTTATAT TCATGTGAAT TTGTAACAAA AATATTTTGT GGATGTTTTC 
    TATAGATTTA TTGTTTCAAC

    Gene models with high sequence identity

    1. AT4G20260.4 [+] show detail - plasma-membrane associated cation-binding protein 1
      E-value: 0; Score: 99.63

     

    InterPro Scan Digest

    See the detailed result >

    AT4G20260.4

    Model type
    Protein coding
    Short Description
    plasma-membrane associated cation-binding protein 1
    Curator Summary
    Encodes a Ca2+ and Cu2+ binding protein. N-terminal myristylation on glycine 2 appears to enable it to associate tightly with the plasma membrane. Recombinant PCaP1 interacts strongly with phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2) and PtdIns (3,4,5)P3, and weakly with PtdIns(3,5)P2 and PtdIns(4,5). It also interacts with calmodulin (CaM) in a calcium-dependent manner. CaM does not interfere with PCaP1 membrane localization but does weaken interactions between it and the PtdInsPs. PCaP1 has an apparent Kd of 10 uM for Cu2+ and can bind six ions per protein. Transcript levels for PCaP1 first fall and then rise following exposure to CuCl2. Mannitol, sorbitol, and the flg22 oligopeptide also increase expression levels.
    Computational Description
    plasma-membrane associated cation-binding protein 1 (PCAP1); FUNCTIONS IN: calmodulin binding, copper ion binding, phosphatidylinositol-3,4,5-trisphosphate binding, phosphatidylinositol-3,5-bisphosphate binding, calcium ion binding; INVOLVED IN: response to cold, N-terminal peptidyl-glycine N-myristoylation, defense response to bacterium; LOCATED IN: plasma membrane, vacuole; EXPRESSED IN: 35 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: DREPP plasma membrane polypeptide (InterPro:IPR008469); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR