F16718

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F16718

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F16718

Determination of introduced cDNA(s)

  • RAFL05-03-H12 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-03-H12 - Resource: pda00743

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-03-H12 - full reading 
    AAAAAAACTC TTAAACAGCT TCCTAACGAG AGGAAACTGA GGAACACAAC 
    AATGGAGTTT CGTGGAGATG CCAACCAGAG GATTGCTAGG ATTTCAGCTC 
    ATCTCACTCC TCAGATGGAG GCCAAGAACT CTGTAATCGG ACGGGAAAAC 
    TGCAGAGCTA AAGGTGGTAA TCCAGGATTC AAAGTAGCAA TTCTTGGAGC 
    TGCAGGTGGA ATTGGACAAT CTTTATCTTT GCTGATGAAG ATGAACCCTC 
    TTGTCTCTTT ACTTCATCTC TACGATGTTG TCAATGCTCC TGGCGTCACT 
    GCTGACGTCA GTCATATGGA CACTGGAGCT GTTGTCCGCG GGTTCTTGGG 
    AGCGAAGCAG CTTGAGGACG CGCTAACGGG TATGGATCTT GTGATCATAC 
    CAGCCGGTAT ACCGAGGAAA CCAGGGATGA CCCGCGATGA TCTGTTTAAA 
    ATCAATGCTG GGATTGTTAA AACACTATGT GAAGGTGTAG CAAAATGTTG 
    TCCTAATGCT ATTGTCAACT TGATCAGCAA CCCTGTGAAC TCTACTGTCC 
    CCATTGCCGC TGAGGTTTTC AAGAAAGCTG GAACTTATGA TCCTAAGAAG 
    CTCCTTGGAG TTACTACACT CGATGTTGCT CGTGCCAACA CATTTGTGGC 
    AGAAGTTCTT GGCCTTGATC CAAGAGAAGT CGATGTGCCA GTAGTTGGGG 
    GACACGCCGG AGTCACAATC TTGCCACTAC TGTCACAGGT TAAACCTCCT 
    AGCAGCTTCA CACCTCAAGA AATTGAGTAC CTGACAAACC GGATTCAAAA 
    TGGTGGAACT GAAGTTGTGG AGGCAAAAGC TGGAGCTGGT TCTGCAACAC 
    TTTCAATGGC ATATGCTGCA GCCAAGTTTG CAGATGCTTG CCTTCGCGGG 
    TTAAGAGGAG ATGCGAATGT CGTAGAATGC TCTTTTGTTG CTTCACAGGT 
    GACAGAATTA GCTTTCTTTG CAACAAAAGT GCGCCTTGGC CGTACAGGAG 
    CAGAGGAAGT GTATCAGCTT GGACCCTTAA ACGAATACGA AAGGATTGGT 
    CTGGAGAAAG CAAAAGATGA ATTAGCCGGA AGTATTCAGA AAGGTGTTGA 
    ATTCATCAGA AAATGAAACT GAGAGATAAT CAGAGAGATA CAATAAGTTA 
    TTTCCTCAAC TATATGATCA TGTACTCATC ATCACATCAT GCCTATGTCT 
    CCTCTGCTTC TGATACAACT TTGTATAAAT CCTTATCAGT TTGTGTACGA 
    TATATGTGAC CC

    Gene models with high sequence identity

    1. AT5G09660.1 [+] show detail - peroxisomal NAD-malate dehydrogenase 2
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMTigr Malate dehydrogenase, NAD-dependent, eukaryote/gamma proteobacteria 9e-176
    HMMPIR L-lactate/malate dehydrogenase 2.9e-55
    Gene3D NAD(P)-binding domain 3.3e-41
    Gene3D Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal 2.6e-59
    superfamily Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal 2.7e-54
    superfamily NAD(P)-binding domain 9e-41
    PatternScan Malate dehydrogenase, active site 0
    HMMPfam Lactate/malate dehydrogenase, C-terminal 3.7e-49
    HMMPfam Lactate/malate dehydrogenase, N-terminal 2e-47
    HMMPanther Malate dehydrogenase, NAD-dependent, eukaryote/gamma proteobacteria 8.1e-195
    HMMPanther MALATE AND LACTATE DEHYDROGENASE 8.1e-195

    See the detailed result >

    AT5G09660.1

    Model type
    Protein coding
    Short Description
    peroxisomal NAD-malate dehydrogenase 2
    Curator Summary
    encodes a microbody NAD-dependent malate dehydrogenase encodes an peroxisomal NAD-malate dehydrogenase that is involved in fatty acid beta-oxidation through providing NAD to the process of converting fatty acyl CoA to acetyl CoA.
    Computational Description
    peroxisomal NAD-malate dehydrogenase 2 (PMDH2); FUNCTIONS IN: malate dehydrogenase activity; INVOLVED IN: regulation of fatty acid beta-oxidation, regulation of photorespiration; LOCATED IN: in 6 components; EXPRESSED IN: 24 plant structures; EXPRESSED DURING: 14 growth stages; CONTAINS InterPro DOMAIN/s: Lactate/malate dehydrogenase, N-terminal (InterPro:IPR001236), Lactate/malate dehydrogenase, C-terminal (InterPro:IPR022383), NAD(P)-binding domain (InterPro:IPR016040), Malate dehydrogenase, NAD-dependent, eukaryote/gamma proteobacteria (InterPro:IPR010097), L-lactate/malate dehydrogenase (InterPro:IPR001557), Malate dehydrogenase, active site (InterPro:IPR001252), Lactate dehydrogenase/glycoside hydrolase, family 4, C-terminal (InterPro:IPR015955); BEST Arabidopsis thaliana protein match is: peroxisomal NAD-malate dehydrogenase 1 (TAIR:AT2G22780.1); Has 17070 Blast hits to 17068 proteins in 5416 species: Archae - 235; Bacteria - 12016; Metazoa - 1211; Fungi - 533; Plants - 741; Viruses - 0; Other Eukaryotes - 2334 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR