F17916

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F17916

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1  
stem (PO:0009047)
  • present in fewer numbers in organism (PATO:0001997)
    (Stem::Number::few)
  • abnormal (PATO:0000460)
    (Stem::Others)
T2  
cauline leaf (PO:0000013)
  • decreased size (PATO:0000587)
    (Cauline leaf::Shape::small)
  • dark green (PATO:0001249)
    (Cauline leaf::Color::dark)
rosette leaf (PO:0000014)
  • present in greater numbers in organism (PATO:0000470)
    (Rosette leaf::Number::many)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
stem (PO:0009047)
  • present in fewer numbers in organism (PATO:0001997)
    (Stem::Number::few)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F17916

Determination of introduced cDNA(s)

  • RAFL09-14-H22 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL09-14-H22 - Resource: pda01774

    full reading [+] show sequence

    Nucleotide sequence: RAFL09-14-H22 - full reading
    AAAAACATAA AGAATCAGCA AAGCAAAATT CGAAGAGACA CAAAAAAACC 
    TTCGTTTGTG TCTCAGAATT TACAAAGTTT TGAGTCAGTA GAGAAATGGC
    GGGAATCGTG ACAGAGCCGT GGTCAGTAGC TGAGAACGGA AACCCAAGCA
    TAACGGCGAA AGGATCGAGC AGAGAACTAA GACTTGGAAG AACCGCTCAC
    AACATGTCTT CTTCTTCTTT GAGGAAAAAA TCAGACCTCC GAGTGATTCA
    GAAGGTTCCA TACAAAGGTC TTAAAGATTT TCTCTCTAAT CTCCAAGAAG
    TCATTCTCGG AACAAAGCTT GCCATTCTTT TTCCGGCCAT TCCTGCCGCC
    ATTATTTGCA CCTATTGTGG CGTCAGTCAG CCTTGGATAT TTGGACTTAG
    CTTGCTAGGA CTGACACCTT TGGCTGAGCG AGTCAGCTTT TTGACAGAGC
    AACTAGCTTT CTACACCGGT CCTACATTGG GTGGTCTATT GAACGCAACG
    TGTGGAAACG CGACTGAATT GATAATCGCG ATTCTTGCTT TGACCAATAA
    CAAAGTCGCA GTGGTGAAAT ATTCGCTGCT AGGTTCGATT TTGTCGAACC
    TTTTATTGGT TCTAGGGACT TCACTCTTCT GTGGAGGAAT CGCTAATATC
    CGAAGGGAAC AGCGGTTCGA CCGGAAACAA GCCGATGTGA ACTTCTTCTT
    ACTTCTACTG GGTTTCTTGT GTCACTTGCT GCCATTGTTG GTGGGATACT
    TGAAAAACGG AGAGGCTTCG GCTGCTGTTT TGTCCGACAT GCAACTGAGT
    ATATCGCGAG GCTTCAGTAT TGTTATGTTG ATCAGCTACA TTGCATATCT
    TGTTTTCCAA CTGTGGACTC ACCGCCAATT GTTCGATGCA CAAGAACAGG
    AAGATGAGTA TGATGACGAT GTGGAGCAAG AAACCGCGGT GATTAGTTTT
    TGGAGCGGTT TTGCATGGTT GGTTGGGATG ACACTCGTTA TCGCATTGCT
    ATCGGAGTAT GTTGTAGCCA CGATTGAGGA AGCATCGGAT AAATGGAACT
    TATCAGTGAG TTTCATAAGC ATCATATTGC TTCCTATTGT TGGAAATGCA
    GCTGAACATG CTGGAGCCGT TATTTTTGCC TTTAAGAACA AGCTTGACAT
    ATCTTTGGGA GTTGCGTTAG GCTCTGCGAC TCAGATTGGC TTATTCGTTG
    TCCCCTTGAC CATCATCGTG GCGTGGATTT TGGGAATTAA TATGGATCTC
    AATTTTGGTC CCCTCGAAAC TGGCTGTCTT GCTGTTTCCA TAATCATCAC
    AGCGTTCACA TTACAGGATG GGAGTTCTCA CTACATGAAG GGACTGGTCC
    TCTTGCTTTG CTATTTCATT ATTGCCATCT GTTTCTTCGT CGACAAACTT
    CCCCAGAAAC AAAATGCCAT TCACTTGGGA CATCAAGCGA TGAACAATGT
    CGTCACTGCA ACAGGAGGAG GAGTTTTCTC ATCTTAAACG AAGACAAGTA
    CCAATGTGTT GAAACAAAAT TTCCAAAGCG AGAGACAATG GAGAAAGGAA
    AACAAAAACC AATTTTCTTG ATTCATTTAT AAATATTGAA AGATTTGTCA
    GAGAGATTCC TGAGATTCAG TGAGTATTAT TTCATTTTTC AAAAATGTTG
    GGTGTCTTGA TGAAGATGAG TTTTGAGAAC TTTATAAATT CTTCTTGTGG
    GTGGGTTTTG CAATGAACTA TCTATGAATG TCTTGTGTCA AATGTTCTAT
    CTCTTTTTAT TAATGGTTTT ATCATTTTTT CTGAGG

    Gene models with high sequence identity

    1. AT2G38170.1 [+] show detail - cation exchanger 1
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMTigr Calcium/proton exchanger 3.7e-237
    HMMTigr Calcium/proton exchanger superfamily 2.5e-88
    HMMPfam Sodium/calcium exchanger membrane region 6.1e-22
    HMMPfam Sodium/calcium exchanger membrane region 2.1e-18

    See the detailed result >

    AT2G38170.1

    Model type
    Protein coding
    Short Description
    cation exchanger 1
    Curator Summary
    Encodes a high affinity vacuolar calcium antiporter. The residue His 338 is critical to Ca2+ transport activity. Disruption of CAX1 reduces manganese and zinc of shoot tissue and results in a decrease in the activity of vacuolar V-type proton ATPase.
    Computational Description
    cation exchanger 1 (CAX1); FUNCTIONS IN: calcium:cation antiporter activity, calcium ion transmembrane transporter activity, calcium:hydrogen antiporter activity; INVOLVED IN: cold acclimation, response to salt stress, cellular zinc ion homeostasis, cellular manganese ion homeostasis, calcium ion transport; LOCATED IN: plant-type vacuole membrane, vacuolar membrane, vacuole, membrane; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Sodium/calcium exchanger membrane region (InterPro:IPR004837), Calcium/proton exchanger superfamily (InterPro:IPR004798), Calcium/proton exchanger (InterPro:IPR004713); BEST Arabidopsis thaliana protein match is: cation exchanger 3 (TAIR:AT3G51860.1); Has 3042 Blast hits to 2882 proteins in 950 species: Archae - 18; Bacteria - 1703; Metazoa - 62; Fungi - 722; Plants - 217; Viruses - 0; Other Eukaryotes - 320 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR