F18509

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F18509

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
rosette leaf (PO:0000014)
  • increased size (PATO:0000586)
    (Rosette leaf::Shape::large)
  • present in greater numbers in organism (PATO:0000470)
    (Rosette leaf::Number::many)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
stem internode (PO:0020142)
  • decreased length (PATO:0000574)
    (Stem::Shape::short internode)
T2  
rosette leaf (PO:0000014)
  • desaturated green (PATO:0001272)
    (Rosette leaf::Color::pale)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F18509

Determination of introduced cDNA(s)

  • RAFL05-16-K10 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-16-K10 - Resource: pda03026

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-16-K10 - full reading 
    AACAGAGAAT CACGAAATCA CGACCGCATT AGAGAAGAAA GAGAGAGAGA 
    GAGCTTTTCT TCTTCTTCTT CCGCTCACTA GATCTGCTCC CTCTCGCAGT 
    CTCCTGGGTT TTGGCAAATT GCTTTTGATT CTTCTCGGGT GGCAGCTATG 
    GTTTCCTCCA CCAAACTCAA ATCCGTTGAT TTCTACAGGA AAATTCCGAG 
    AGATTTGACG GAGGCATCTC TATCAGGCGC TGGGTTATCA ATTGTAGCTG 
    CTCTCTTTAT GATGTTTTTG TTTGGAATGG AGCTGAGTAG TTATTTGGAA 
    GTTAACACTA CCACAGCTGT TATAGTTGAC AAGAGCAGTG ATGGTGACTT 
    CTTACGCATT GATTTCAACA TCAGTTTTCC TGCTCTTTCC TGTGAATTTG 
    CATCCGTTGA TGTGAGCGAT GTCTTGGGAA CGAATAGGTT GAATATAACA 
    AAAACAGTTC GCAAGTTTCC AATTGATCCA CATTTAAGGT CCACCGGCGC 
    AGAATTCCAC TCTGGCCTTG CATTACATAA CATAAACCAT GGAGAAGAAA 
    CTAAGGAAGA GTTTCCTGAT GGTGCTATAC CATTGACCAG TGCTAGTTTT 
    GAAGCATTAT CACATCACTT TCCAATATTG GTTGTTAACT TTAATGCACC 
    ATGGTGCTAC TGGAGTAATC GTCTGAAACC TTCATGGGAG AAAGCAGCTA 
    ATATAATAAA ACAAAGATAT GATCCTGAAG CTGATGGCCG TGTTCTTCTA 
    GGAAATGTTG ATTGCACGGA AGAACCTGCG CTATGTAAGA GGAATCACAT 
    ACAAGGCTAT CCATCTATTC GTATTTTCCG CAAAGGCAGT GACCTTAGAG 
    AGGACCATGG ACACCACGAA CATGAATCTT ACTATGGAGA TCGGGACACA 
    GACAGTATAG TTAAGATGGT GGAGGGACTG GTCGCACCTA TCCACCCGGA 
    GACTCACAAG GTGGCTTTGG ATGGAAAATC CAATGATACT GTAAAACATC 
    TTAAAAAGGG ACCAGTTACA GGAGGTTGTA GAGTTGAAGG TTATGTACGT 
    GTAAAGAAGG TTCCAGGAAA TCTCGTGATC TCAGCTCATT CAGGAGCTCA 
    TTCATTTGAT TCTTCTCAAA TGAACATGTC GCATGTTGTT TCTCATTTTT 
    CGTTTGGGAG GATGATTTCT CCTAGGCTGC TGACCGATAT GAAGCGATTG 
    TTGCCATATC TTGGCCTAAG CCATGACAGG CTGGATGGAA AGGCATTCAT 
    AAATCAACAT GAGTTTGGTG CCAATGTCAC TATCGAACAC TACCTTCAAA 
    CAGTCAAAAC AGAGGTCATT ACAAGAAGAT CCGGTCAAGA ACATTCACTG 
    ATAGAGGAAT ATGAATACAC CGCTCACAGC AGCGTGGCTC AGACTTACTA 
    CTTACCCGTT GCGAAGTTTC ACTTTGAACT CTCTCCTATG CAGATCTTAA 
    TAACCGAAAA CCCGAAGTCC TTCTCACACT TCATCACAAA TCTGTGTGCC 
    ATTATTGGTG GTGTTTTCAC GGTTGCCGGA ATACTAGATT CGATTTTTCA 
    CAATACAGTA AGACTGGTAA AAAAGGTCGA ACTTGGGAAA AACATTTGAT 
    CGTCCACTGG AGGTTAGAAT TTTCCATTAA TATATAAAAC CATAGAAGAA 
    CCTCAGACTG TTTACTTTGA ATAGCTGATC TACACAGAAA AACTCAAAAA 
    AAGAGAGAAT TTTTGTTTGA CACAATCTTA GTTTCTTTAT GGTTCTTGTT 
    CTATTAAAAT CCGCCTGGTT TTTCGT

    Gene models with high sequence identity

    1. AT3G20560.1 [+] show detail - PDI-like 5-3
      E-value: 0; Score: 99.94

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Protein of unknown function DUF1692 3.4e-27
    HMMPfam Thioredoxin domain 0.00000000000013
    HMMPanther THIOREDOXIN DOMAIN-CONTAINING 1.2e-303
    HMMPanther SEROLOGICALLY DEFINED BREAST CANCER ANTIGEN N 1.2e-303
    ProfileScan Thioredoxin-like 10.271
    superfamily Thioredoxin-like fold 2e-24
    Gene3D Thioredoxin fold 3.8e-18

    See the detailed result >

    AT3G20560.1

    Model type
    Protein coding
    Short Description
    PDI-like 5-3
    Curator Summary
    Encodes a protein disulfide isomerase-like (PDIL) protein, a member of a multigene family within the thioredoxin (TRX) superfamily. Unlike several other PDI family members, transcript levels for this gene are not up-regulated in response to three different chemical inducers of ER stress (dithiothreitol, beta-mercaptoethanol, and tunicamycin).
    Computational Description
    PDI-like 5-3 (PDIL5-3); FUNCTIONS IN: protein disulfide isomerase activity; INVOLVED IN: cell redox homeostasis; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Thioredoxin fold (InterPro:IPR012335), Thioredoxin-like (InterPro:IPR017936), Thioredoxin domain (InterPro:IPR013766), Thioredoxin-like fold (InterPro:IPR012336), Protein of unknown function DUF1692 (InterPro:IPR012936); BEST Arabidopsis thaliana protein match is: Thioredoxin protein with domain of unknown function (DUF1692) (TAIR:AT1G50950.1); Has 5147 Blast hits to 3670 proteins in 375 species: Archae - 8; Bacteria - 112; Metazoa - 2494; Fungi - 904; Plants - 886; Viruses - 3; Other Eukaryotes - 740 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR