F18509

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F18509

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1  
rosette leaf (PO:0000014)
  • increased size (PATO:0000586)
    (Rosette leaf::Shape::large)
  • present in greater numbers in organism (PATO:0000470)
    (Rosette leaf::Number::many)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
stem internode (PO:0020142)
  • decreased length (PATO:0000574)
    (Stem::Shape::short internode)
T2  
rosette leaf (PO:0000014)
  • desaturated green (PATO:0001272)
    (Rosette leaf::Color::pale)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F18509

Determination of introduced cDNA(s)

  • RAFL05-16-K10 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-16-K10 - Resource: pda03026

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-16-K10 - full reading
    AACAGAGAAT CACGAAATCA CGACCGCATT AGAGAAGAAA GAGAGAGAGA 
    GAGCTTTTCT TCTTCTTCTT CCGCTCACTA GATCTGCTCC CTCTCGCAGT
    CTCCTGGGTT TTGGCAAATT GCTTTTGATT CTTCTCGGGT GGCAGCTATG
    GTTTCCTCCA CCAAACTCAA ATCCGTTGAT TTCTACAGGA AAATTCCGAG
    AGATTTGACG GAGGCATCTC TATCAGGCGC TGGGTTATCA ATTGTAGCTG
    CTCTCTTTAT GATGTTTTTG TTTGGAATGG AGCTGAGTAG TTATTTGGAA
    GTTAACACTA CCACAGCTGT TATAGTTGAC AAGAGCAGTG ATGGTGACTT
    CTTACGCATT GATTTCAACA TCAGTTTTCC TGCTCTTTCC TGTGAATTTG
    CATCCGTTGA TGTGAGCGAT GTCTTGGGAA CGAATAGGTT GAATATAACA
    AAAACAGTTC GCAAGTTTCC AATTGATCCA CATTTAAGGT CCACCGGCGC
    AGAATTCCAC TCTGGCCTTG CATTACATAA CATAAACCAT GGAGAAGAAA
    CTAAGGAAGA GTTTCCTGAT GGTGCTATAC CATTGACCAG TGCTAGTTTT
    GAAGCATTAT CACATCACTT TCCAATATTG GTTGTTAACT TTAATGCACC
    ATGGTGCTAC TGGAGTAATC GTCTGAAACC TTCATGGGAG AAAGCAGCTA
    ATATAATAAA ACAAAGATAT GATCCTGAAG CTGATGGCCG TGTTCTTCTA
    GGAAATGTTG ATTGCACGGA AGAACCTGCG CTATGTAAGA GGAATCACAT
    ACAAGGCTAT CCATCTATTC GTATTTTCCG CAAAGGCAGT GACCTTAGAG
    AGGACCATGG ACACCACGAA CATGAATCTT ACTATGGAGA TCGGGACACA
    GACAGTATAG TTAAGATGGT GGAGGGACTG GTCGCACCTA TCCACCCGGA
    GACTCACAAG GTGGCTTTGG ATGGAAAATC CAATGATACT GTAAAACATC
    TTAAAAAGGG ACCAGTTACA GGAGGTTGTA GAGTTGAAGG TTATGTACGT
    GTAAAGAAGG TTCCAGGAAA TCTCGTGATC TCAGCTCATT CAGGAGCTCA
    TTCATTTGAT TCTTCTCAAA TGAACATGTC GCATGTTGTT TCTCATTTTT
    CGTTTGGGAG GATGATTTCT CCTAGGCTGC TGACCGATAT GAAGCGATTG
    TTGCCATATC TTGGCCTAAG CCATGACAGG CTGGATGGAA AGGCATTCAT
    AAATCAACAT GAGTTTGGTG CCAATGTCAC TATCGAACAC TACCTTCAAA
    CAGTCAAAAC AGAGGTCATT ACAAGAAGAT CCGGTCAAGA ACATTCACTG
    ATAGAGGAAT ATGAATACAC CGCTCACAGC AGCGTGGCTC AGACTTACTA
    CTTACCCGTT GCGAAGTTTC ACTTTGAACT CTCTCCTATG CAGATCTTAA
    TAACCGAAAA CCCGAAGTCC TTCTCACACT TCATCACAAA TCTGTGTGCC
    ATTATTGGTG GTGTTTTCAC GGTTGCCGGA ATACTAGATT CGATTTTTCA
    CAATACAGTA AGACTGGTAA AAAAGGTCGA ACTTGGGAAA AACATTTGAT
    CGTCCACTGG AGGTTAGAAT TTTCCATTAA TATATAAAAC CATAGAAGAA
    CCTCAGACTG TTTACTTTGA ATAGCTGATC TACACAGAAA AACTCAAAAA
    AAGAGAGAAT TTTTGTTTGA CACAATCTTA GTTTCTTTAT GGTTCTTGTT
    CTATTAAAAT CCGCCTGGTT TTTCGT

    Gene models with high sequence identity

    1. AT3G20560.1 [+] show detail - PDI-like 5-3
      E-value: 0; Score: 99.94

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Protein of unknown function DUF1692 3.4e-27
    HMMPfam Thioredoxin domain 1.3e-13
    HMMPanther THIOREDOXIN DOMAIN-CONTAINING 1.2e-303
    HMMPanther SEROLOGICALLY DEFINED BREAST CANCER ANTIGEN N 1.2e-303
    ProfileScan Thioredoxin-like 10.271
    superfamily Thioredoxin-like fold 2e-24
    Gene3D Thioredoxin fold 3.8e-18

    See the detailed result >

    AT3G20560.1

    Model type
    Protein coding
    Short Description
    PDI-like 5-3
    Curator Summary
    Encodes a protein disulfide isomerase-like (PDIL) protein, a member of a multigene family within the thioredoxin (TRX) superfamily. Unlike several other PDI family members, transcript levels for this gene are not up-regulated in response to three different chemical inducers of ER stress (dithiothreitol, beta-mercaptoethanol, and tunicamycin).
    Computational Description
    PDI-like 5-3 (PDIL5-3); FUNCTIONS IN: protein disulfide isomerase activity; INVOLVED IN: cell redox homeostasis; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Thioredoxin fold (InterPro:IPR012335), Thioredoxin-like (InterPro:IPR017936), Thioredoxin domain (InterPro:IPR013766), Thioredoxin-like fold (InterPro:IPR012336), Protein of unknown function DUF1692 (InterPro:IPR012936); BEST Arabidopsis thaliana protein match is: Thioredoxin protein with domain of unknown function (DUF1692) (TAIR:AT1G50950.1); Has 5147 Blast hits to 3670 proteins in 375 species: Archae - 8; Bacteria - 112; Metazoa - 2494; Fungi - 904; Plants - 886; Viruses - 3; Other Eukaryotes - 740 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR