F18606

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F18606

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F18606

Determination of introduced cDNA(s)

  • RAFL06-11-P04 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL06-11-P04 - Resource: pda07545

    full reading [+] show sequence

    Nucleotide sequence: RAFL06-11-P04 - full reading 
    AACATACACA AAACACTAAA AGTAGAAGAA AAATGGCGAC TCTTAAGGTT 
    TCTGATTCTG TTCCTGCTCC TTCTGATGAT GCTGAGCAAT TGAGAACCGC 
    TTTTGAAGGA TGGGGTACGA ACGAGGACTT GATCATATCA ATCTTGGCTC 
    ACAGAAGTGC TGAACAGAGG AAAGTCATCA GGCAAGCATA CCACGAAACC 
    TACGGCGAAG ACCTTCTCAA GACTCTTGAC AAGGAGCTCT CTAACGATTT 
    CGAGAGAGCT ATCTTGTTGT GGACTCTTGA ACCCGGTGAG CGTGATGCTT 
    TATTGGCTAA TGAAGCTACA AAAAGATGGA CTTCAAGCAA CCAAGTTCTT 
    ATGGAAGTTG CTTGCACAAG GACATCAACG CAGCTGCTTC ACGCTAGGCA 
    AGCTTACCAT GCTCGCTACA AGAAGTCTCT TGAAGAGGAC GTTGCTCACC 
    ACACTACCGG TGACTTCAGA AAGCTTTTGG TTTCTCTTGT TACCTCATAC 
    AGGTACGAAG GAGATGAAGT GAACATGACA TTGGCTAAGC AAGAAGCTAA 
    GCTGGTCCAT GAGAAAATCA AGGACAAGCA CTACAATGAT GAGGATGTTA 
    TTAGAATCTT GTCCACAAGA AGCAAAGCTC AGATCAATGC TACTTTTAAC 
    CGTTACCAAG ATGATCATGG CGAGGAAATT CTCAAGAGTC TTGAGGAAGG 
    AGATGATGAT GACAAGTTCC TTGCACTTTT GAGGTCAACC ATTCAGTGCT 
    TGACAAGACC AGAGCTTTAC TTTGTCGATG TTCTTCGTTC AGCAATCAAC 
    AAAACTGGAA CTGATGAAGG AGCACTCACT AGAATTGTGA CCACAAGAGC 
    TGAGATTGAC TTGAAGGTCA TTGGAGAGGA GTACCAGCGC AGGAACAGCA 
    TTCCTTTGGA GAAAGCTATT ACCAAAGACA CTCGTGGAGA TTACGAGAAG 
    ATGCTCGTCG CACTTCTCGG TGAAGATGAT GCTTAATCAA TCAATCCTCC 
    ACAGAGAAAC ATAAGCTGCT CTACAGCTTC TGTTATCTCT TATCTCCCTC 
    TCTCTCTCTT TGATGAGTTT CAAATCGTTT GATTTTGTTT CTACAAAAAC 
    CTTGTTTGTT TCTGTTGTGT GTTTTGAGTT CCTAAATAAT GCAAAAGAGA 
    GAGACAGAGA GAACCAGTGT GGTCTCTTAA GTTATATATA TATGAAGAGC 
    ATTGGCCC

    Gene models with high sequence identity

    1. AT1G35720.1 [+] show detail - annexin 1
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Annexin repeat 1.3e-23
    HMMPfam Annexin repeat 1.3e-20
    HMMPfam Annexin repeat 1.3e-13
    HMMPfam Annexin repeat 7.1e-21
    superfamily Annexin 2.9e-102
    HMMSmart Annexin repeat 5e-22
    HMMSmart Annexin repeat 1.1e-16
    HMMSmart Annexin repeat 0.088
    HMMSmart Annexin repeat 5.2e-21
    FPrintScan Annexin, type plant 8.2e-24
    FPrintScan Annexin, type plant 8.2e-24
    FPrintScan Annexin, type plant 8.2e-24
    FPrintScan Annexin 5e-22
    FPrintScan Annexin 5e-22
    FPrintScan Annexin 5e-22
    FPrintScan Annexin 5e-22
    FPrintScan Annexin 5e-22
    Gene3D Annexin repeat 4.9e-21
    Gene3D Annexin repeat 4.4e-18
    Gene3D Annexin repeat 1.8e-24
    Gene3D Annexin repeat 3.2e-19
    HMMPanther Annexin like protein 1.4e-197
    HMMPanther Annexin 1.4e-197
    PatternScan Annexin repeat, conserved site 0

    See the detailed result >

    AT1G35720.1

    Model type
    Protein coding
    Short Description
    annexin 1
    Curator Summary
    Encodes a member of the annexin gene family, a diverse, multigene family of calcium-dependent, membrane-binding proteins. The protein was determined to have peroxidase activity. This activity is thought to be dependent on the presence of post-translational modifications (most likely phosphorylation). The protein was shown to be present as a mixture of monomer and homodimer. The homodimerization seems to be dependent on the presence of Ca2+ or H2O2. The dimerization was prevented by the addition of DTT, β-mercaptoethanol and TCEP. Annat1 mRNA is expressed in flowers, roots,leaves and stems and is most abundant in stems. mRNA levels are increased in response to oxidative stress. Developmental expression patterns suggest a role in Golgi-mediated polysaccharide secretion.
    Computational Description
    annexin 1 (ANNAT1); FUNCTIONS IN: in 7 functions; INVOLVED IN: in 8 processes; LOCATED IN: in 10 components; EXPRESSED IN: 31 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: Annexin like protein (InterPro:IPR015472), Annexin repeat (InterPro:IPR018502), Annexin repeat, conserved site (InterPro:IPR018252), Annexin (InterPro:IPR001464), Annexin, type plant (InterPro:IPR009118); BEST Arabidopsis thaliana protein match is: annexin 2 (TAIR:AT5G65020.1); Has 3809 Blast hits to 2217 proteins in 205 species: Archae - 0; Bacteria - 0; Metazoa - 2893; Fungi - 160; Plants - 482; Viruses - 0; Other Eukaryotes - 274 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR