F19145

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F19145

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
cauline leaf (PO:0000013)
  • increased size (PATO:0000586)
    (Cauline leaf::Shape::large)
rosette leaf (PO:0000014)
  • present in greater numbers in organism (PATO:0000470)
    (Rosette leaf::Number::many)
fruit (PO:0009001)
  • increased length (PATO:0000573)
    (Silique::Shape::long)
stem (PO:0009047)
  • dark green (PATO:0001249)
    (Stem::Color::dark)
  • abnormal (PATO:0000460)
    (Stem::Others)
T2  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
cauline leaf (PO:0000013)
  • decreased size (PATO:0000587)
    (Cauline leaf::Shape::small)
  • dark green (PATO:0001249)
    (Cauline leaf::Color::dark)
rosette leaf (PO:0000014)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
  • present in greater numbers in organism (PATO:0000470)
    (Rosette leaf::Number::many)
stem (PO:0009047)
  • dark green (PATO:0001249)
    (Stem::Color::dark)
  • abnormal (PATO:0000460)
    (Stem::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F19145

Determination of introduced cDNA(s)

  • RAFL04-16-C20 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL04-16-C20 - Resource: pda00586

    full reading [+] show sequence

    Nucleotide sequence: RAFL04-16-C20 - full reading 
    ATGTATTCTT CTCCTTGTCC TTCCTTACTC TCTATATTTT TCTCAATTCT 
    CCGATTTGAT CCAACGATTT CACAGATCTC TGATCTAAAT GGCATCCAAC 
    GGTGATAAAG GACTGATCGT TAGCTTCGGC GAGATGCTAA TCGATTTCGT 
    CCCTACCGAA TCAGGCGTCT CTCTCGCCGA AGCTCCTGGA TTCCTCAAAG 
    CTCCAGGCGG TGCTCCCGCT AACGTCGCAA TCGCTGTTTC TCGTCTCGGT 
    GGACGATCTG CTTTCGTCGG AAAACTTGGA GACGACGAGT TCGGTCATAT 
    GTTGGCTGGG ATTTTGAGGA AGAACGGTGT TGATGATCAG GGGATTAACT 
    TCGATACTGG AGCTAGAACT GCTTTAGCTT TCGTTACGTT ACGTGCTGAT 
    GGAGATCGTG AGTTTATGTT TTACCACAAT CCTAGCGCCG ATATGCTTCT 
    TCGTCCTGAT GAACTCAATC TCGACCTCAT CAGATCCGCG AAAGTGTTTC 
    ACTATGGATC AATAAGTTTG ATAGTGGAGC CGTGTAGGTC AGCTCATTTG 
    AAGGCCATGG AAGTGGCGAA AGAAGCAGGA GCTCTTCTTT CCTATGACCC 
    AAACCTCAGG GAGCCTCTGT GGCCATCAAA AGAAGAAGCC AAAACACAGA 
    TCATGAGCAT TTGGGACAAA GCTGAGATCA TCAAGGTGAG CGATGTTGAG 
    CTTGAGTTTC TAACTGGAAG CAACAAGATT GATGATGAGA CCGCGTTGAC 
    CTTGTGGCAT CCCAACTTGA AGCTGTTGTT AGTCACTCTT GGTGAAAAGG 
    GCTGTCGGTA TTACACCAAG ACTTTCAAAG GAGCCGTTGA CCCTTTCCAT 
    GTGAACGCTG TGGACACAAC CGGAGCTGGA GATTCCTTTG TCGGTGCTCT 
    TCTAAACCAG ATTGTCGATG ATCGATCCGT TCTCGAGGAC GAAGAGAGAT 
    TGAGGAAAGT GCTGAGATTC GCAAATGCTT GTGGAGCAAT CACAACGACC 
    AAAAAAGGAG CCATTCCAGC TCTTCCCTCA GATGCTGAAG TTCGGAGCTT 
    TCTCGAAAAG AAATAGAAAC AAAACAAACT CAAGCTTCTA TTACGTAATC 
    TGTTTCCGCG ATTATCTTCT CTTTTTGTAT TCTTGTTTTC GGATGGTTGA 
    ATCGAGAAGA GAAACAAATT CGGCATTCCG CTCTGTTTTT GTTTCTCTTC 
    TTCAAATGTA ATGATTTAGA AAAGACGTAT GTTGCAATTT AAGGCTTCAC 
    TTGCTAATAA AATGATAAGG TTTTTC

    Gene models with high sequence identity

    1. AT2G31390.1 [+] show detail - pfkB-like carbohydrate kinase family protein
      E-value: 0; Score: 99.84

     

    InterPro Scan Digest

    Program Description E-value
    Gene3D no description 9.2e-91
    FPrintScan Ribokinase 0.0000026
    FPrintScan Ribokinase 0.0000026
    PatternScan Carbohydrate/puine kinase, PfkB, conserved site 0
    PatternScan Carbohydrate/puine kinase, PfkB, conserved site 0
    HMMPanther FRUCTOKINASE 2.6e-145
    HMMPanther SUGAR KINASE RELATED 2.6e-145
    superfamily Ribokinase-like 5.7e-86
    HMMPfam Carbohydrate/purine kinase 2.5e-86

    See the detailed result >

    AT2G31390.1

    Model type
    Protein coding
    Short Description
    pfkB-like carbohydrate kinase family protein
    Computational Description
    pfkB-like carbohydrate kinase family protein; FUNCTIONS IN: kinase activity, ribokinase activity; INVOLVED IN: D-ribose metabolic process, acetate fermentation, sucrose biosynthetic process, sucrose catabolic process, using beta-fructofuranosidase; LOCATED IN: plasma membrane; EXPRESSED IN: 27 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: Carbohydrate/purine kinase (InterPro:IPR011611), Ribokinase (InterPro:IPR002139), Carbohydrate/puine kinase, PfkB, conserved site (InterPro:IPR002173); BEST Arabidopsis thaliana protein match is: pfkB-like carbohydrate kinase family protein (TAIR:AT1G06030.1); Has 18509 Blast hits to 18503 proteins in 2397 species: Archae - 386; Bacteria - 14053; Metazoa - 143; Fungi - 116; Plants - 466; Viruses - 0; Other Eukaryotes - 3345 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR