F19510

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F19510

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1  
cauline leaf (PO:0000013)
  • desaturated green (PATO:0001272)
    (Cauline leaf::Color::pale)
rosette leaf (PO:0000014)
  • desaturated green (PATO:0001272)
    (Rosette leaf::Color::pale)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
fruit (PO:0009001)
  • abnormal (PATO:0000460)
    (Silique::Others)
stem (PO:0009047)
  • desaturated green (PATO:0001272)
    (Stem::Color::pale)
T2  
whole plant (PO:0000003)
  • decreased height (PATO:0000569)
    (Adult plant::Plant height::short)
cauline leaf (PO:0000013)
  • desaturated green (PATO:0001272)
    (Cauline leaf::Color::pale)
rosette leaf (PO:0000014)
  • desaturated green (PATO:0001272)
    (Rosette leaf::Color::pale)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
fruit (PO:0009001)
  • abnormal (PATO:0000460)
    (Silique::Others)
stem (PO:0009047)
  • desaturated green (PATO:0001272)
    (Stem::Color::pale)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F19510

Determination of introduced cDNA(s)

  • RAFL04-15-B17 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL04-15-B17 - Resource: pda00334

    full reading [+] show sequence

    Nucleotide sequence: RAFL04-15-B17 - full reading
    ATCTAACTCT CTCTCTACTG CAATTTTTAG GGTTTTATCC TCCGAAAGTC 
    TCAACCTTTT TCTTATCCTC AACAAGGAGA AATGGCGGCG ACTCAACTTA
    CAGCGTCACC AGTGACTATG TCAGCAAGGA GCTTAGCCTC GCTGGATGGT
    CTCAGAGCTT CGAGTGTCAA GTTTTCATCT TTGAAACCAG GGACCCTTAG
    ACAGAGCCAG TTCCGTCGTT TGGTTGTCAA AGCTGCTTCT GTTGTTGCCC
    CTAAGTATAC TTCAATTAAG CCATTGGGAG ATCGAGTTTT GGTGAAGATC
    AAGGAGGCAG AGGAGAAGAC TTTAGGTGGT ATCTTACTTC CATCCACTGC
    TCAATCAAAA CCTCAAGGAG GTGAAGTCGT TGCCGTGGGT GAAGGAAGAA
    CTATTGGGAA GAACAAAATT GATATCACTG TCCCTACTGG AGCACAAATT
    ATCTACTCCA AATACGCAGG AACTGAGGTG GAGTTCAATG ATGTGAAGCA
    TCTTATCCTC AAGGAAGATG ATATTGTTGG CATTCTTGAG ACAGAGGACA
    TCAAAGATCT CAAACCTTTG AATGACCGAG TCTTTATTAA GGTTGCTGAG
    GCGGAGGAGA AAACAGCTGG AGGGTTGTTG TTAACCGAGA CTACCAAAGA
    GAAGCCTTCT ATTGGCACGG TGATAGCAGT TGGACCGGGT TCCCTAGACG
    AGGAAGGTAA AATTACGCCT CTACCAGTAT CAACCGGAAG CACAGTACTT
    TACTCCAAGT ATGCTGGTAA CGACTTCAAG GGCAAAGATG GTTCCAACTA
    CATTGCCCTC AGAGCTTCAG ATGTGATGGC TATACTTTCT TAGTTATGTT
    ATATCTTTGT AATCTGCAAC TTGTATCCCA ATTGTGGAAA TTTTTTCCGT
    AAACGGCCTG AGCATAATCT GGAATAAAGA CTTGAGTTTG AAAATGTGAT
    TTTATTGAAA AAAAAAAAAA AAA

    Gene models with high sequence identity

    1. AT5G20720.1 [+] show detail - chaperonin 20
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    FPrintScan Chaperonin Cpn10, subgroup 4.6e-25
    FPrintScan Chaperonin Cpn10, subgroup 4.6e-25
    FPrintScan Chaperonin Cpn10, subgroup 4.6e-25
    FPrintScan Chaperonin Cpn10, subgroup 4.6e-25
    HAMAP Chaperonin Cpn10, subgroup 22.586
    HAMAP Chaperonin Cpn10, subgroup 21.083
    HMMPanther Chaperonin Cpn10, subgroup 1.7e-41
    Gene3D Chaperonin Cpn10 2.4e-26
    Gene3D Chaperonin Cpn10 1.8e-23
    HMMSmart Chaperonin Cpn10, subgroup 1.4e-40
    HMMSmart Chaperonin Cpn10, subgroup 1.6e-40
    superfamily GroES-like 2e-31
    superfamily GroES-like 1.1e-29
    PatternScan Chaperonin Cpn10, conserved site 0
    PatternScan Chaperonin Cpn10, conserved site 0
    HMMPIR Chaperonin 21, chloroplast 8.3e-169
    HMMPfam Chaperonin Cpn10 1.9e-27
    HMMPfam Chaperonin Cpn10 1.9e-26

    See the detailed result >

    AT5G20720.1

    Model type
    Protein coding
    Short Description
    chaperonin 20
    Curator Summary
    Encodes a chloroplast co-chaperonin with similarity to CPN21 from spinach, E.coli GroES.
    Computational Description
    chaperonin 20 (CPN20); FUNCTIONS IN: calmodulin binding, copper ion binding; INVOLVED IN: response to cadmium ion, response to cold; LOCATED IN: in 7 components; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Chaperonin Cpn10 (InterPro:IPR020818), GroES-like (InterPro:IPR011032), Chaperonin Cpn10, conserved site (InterPro:IPR018369), Chaperonin 21, chloroplast (InterPro:IPR017416), Chaperonin Cpn10, subgroup (InterPro:IPR001476); BEST Arabidopsis thaliana protein match is: chaperonin 10 (TAIR:AT1G14980.1); Has 18503 Blast hits to 9472 proteins in 2912 species: Archae - 42; Bacteria - 12669; Metazoa - 631; Fungi - 230; Plants - 426; Viruses - 4; Other Eukaryotes - 4501 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR