F19605

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F19605

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T2 seed (PO:0009010)
  • increased size (PATO:0000586)
    (Seed::Shape::large)
  • increased length (PATO:0000573)
    (Seed::Shape::long)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F19605

Determination of introduced cDNA(s)

  • RAFL09-10-L23 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL09-10-L23 - Resource: pda05379

    full reading [+] show sequence

    Nucleotide sequence: RAFL09-10-L23 - full reading
    TGGCAACACA AAAACATACA CACTACTAAT AAACCATGAA GCTTCTTATG 
    CTCGCCTTTG TTTTTCTATT AGCTTTGGCG ACTTGTAAAG GTGACGAGTT
    TGTTTGTGAA GAGAACGAGC CATTCACATG TAACCAAACT AAACTTTTCA
    ACAGTGGCAA TTTCGAAAAA GGCTTCATCT TCGGTGTTGC ATCTTCTGCT
    TACCAGGTGG AAGGCGGTAG AGGCCGTGGA CTTAACGTTT GGGATAGCTT
    CACTCACCGA TTCCCAGAGA AAGGTGGAGC TGATTTGGGA AATGGAGACA
    CTACTTGTGA CTCATATACT CTTTGGCAGA AAGATATAGA CGTGATGGAC
    GAGCTCAACT CTACTGGCTA CAGATTCTCC ATTGCGTGGT CAAGACTCCT
    TCCAAAAGGA AAGAGGAGCA GGGGAGTGAA CCCAGGAGCT ATTAAGTACT
    ACAACGGTCT CATAGATGGC CTCGTCGCAA AGAATATGAC GCCCTTTGTT
    ACCCTCTTTC ATTGGGACCT TCCTCAAACA CTACAAGATG AATATAACGG
    TTTCTTGAAC AAAACGATCG TAGACGATTT CAAGGATTAC GCGGATCTAT
    GTTTCGAGTT ATTTGGTGAT AGGGTAAAGA ACTGGATCAC CATCAACCAG
    CTATACACAG TGCCTACTAG AGGATATGCA TTGGGAACAG ATGCACCCGG
    TCGATGTTCT CCTAAGATTG ACGTTAGATG TCCCGGCGGA AATTCGTCAA
    CAGAACCCTA TATTGTTGCA CATAACCAGC TTCTTGCTCA TGCAGCGGCC
    GTTGATGTTT ACAGGACGAA ATATAAGGAT GACCAAAAAG GTATGATTGG
    ACCAGTGATG ATAACTAGAT GGTTTCTTCC ATTTGATCAT AGTCAAGAGA
    GCAAAGATGC AACTGAGCGG GCTAAAATAT TTTTCCATGG ATGGTTCATG
    GGGCCTCTAA CAGAAGGTAA ATACCCAGAC ATCATGAGGG AATATGTTGG
    TGATCGGCTT CCAGAGTTCA GTGAAACAGA AGCCGCACTT GTAAAGGGTT
    CATATGATTT TCTTGGTCTC AACTATTACG TCACTCAATA CGCCCAAAAT
    AATCAGACGA TTGTTCCTTC GGACGTACAC ACTGCCTTGA TGGACTCACG
    CACAACTCTC ACATCTAAAA ATGCAACTGG TCATGCTCCT GGTCCACCGT
    TCAATGCAGC CAGTTACTAC TACCCAAAAG GCATTTACTA CGTAATGGAT
    TACTTCAAAA CCACTTACGG TGACCCTTTA ATATATGTCA CTGAGAATGG
    ATTTAGTACC GCAGGTGATG AGGACTTTGA GAAGGCTACT GCCGATTACA
    AGCGGATTGA TTATCTCTGT AGTCATCTCT GTTTCCTCAG TAAAGTCATC
    AAGGAGAAGA ATGTCAACGT GAAAGGATAT TTTGCTTGGT CTCTTGGGGA
    TAATTACGAA TTCTGTAACG GATTTACCGT CAGATTCGGA CTAAGTTACG
    TTGATTTCGC AAATATCACT GGTGATAGAG ACCTCAAAGC ATCTGGCAAA
    TGGTTCCAGA AGTTCATAAA CGTTACCGAC GAAGACTCTA CGAACCAAGA
    TCTACTCCGC TCAAGCGTCT CCTCCAAGAA CCGTGATCGG AAGAGTCTTG
    CAGATGCATG AAATATCCAA TCCACTATAT GTCCACCAAG ATCATCTTCA
    TGTTTCCTCT TTCTACTTGC TCCATAGATA AGGAGCTTTT TCTACCATAT
    GTATTAAAAT AAAATCCTAA TAAAAGATGA TCAATAATAA TAAAGACTTT
    GTTAT

    Gene models with high sequence identity

    1. AT5G26000.1 [+] show detail - thioglucoside glucohydrolase 1
      E-value: 0; Score: 99.94

     

    InterPro Scan Digest

    Program Description E-value
    PatternScan Glycoside hydrolase, family 1, active site 0
    PatternScan Glycoside hydrolase, family 1, active site 0
    superfamily Glycoside hydrolase, catalytic core 2.9e-166
    FPrintScan Glycoside hydrolase, family 1 6.8e-22
    FPrintScan Glycoside hydrolase, family 1 6.8e-22
    FPrintScan Glycoside hydrolase, family 1 6.8e-22
    FPrintScan Glycoside hydrolase, family 1 6.8e-22
    FPrintScan Glycoside hydrolase, family 1 6.8e-22
    Gene3D Glycoside hydrolase, subgroup, catalytic core 7.9e-168
    HMMPfam Glycoside hydrolase, family 1 4.6e-156
    HMMPanther BETA-GLUCOSIDASE 6.2e-286
    HMMPanther Glycoside hydrolase, family 1 6.2e-286

    See the detailed result >

    AT5G26000.1

    Model type
    Protein coding
    Short Description
    thioglucoside glucohydrolase 1
    Curator Summary
    member of Glycoside Hydrolase Family 1. encodes one of two known functional myrosinase enzymes in Arabidopsis. The enzyme catalyzes the hydrolysis of glucosinolates into compounds that are toxic to various microbes and herbivores.
    Computational Description
    thioglucoside glucohydrolase 1 (TGG1); FUNCTIONS IN: thioglucosidase activity, beta-glucosidase activity, hydrolase activity, hydrolyzing O-glycosyl compounds; INVOLVED IN: glucosinolate catabolic process; LOCATED IN: in 7 components; EXPRESSED IN: 9 plant structures; EXPRESSED DURING: seedling growth; CONTAINS InterPro DOMAIN/s: Glycoside hydrolase, family 1 (InterPro:IPR001360), Glycoside hydrolase, family 1, active site (InterPro:IPR018120), Glycoside hydrolase, catalytic core (InterPro:IPR017853), Glycoside hydrolase, subgroup, catalytic core (InterPro:IPR013781); BEST Arabidopsis thaliana protein match is: glucoside glucohydrolase 2 (TAIR:AT5G25980.2); Has 11138 Blast hits to 10810 proteins in 1458 species: Archae - 132; Bacteria - 7708; Metazoa - 712; Fungi - 191; Plants - 1422; Viruses - 0; Other Eukaryotes - 973 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR