F19738

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F19738

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
cauline leaf (PO:0000013)
  • increased size (PATO:0000586)
    (Cauline leaf::Shape::large)
rosette leaf (PO:0000014)
  • increased width (PATO:0000600)
    (Rosette leaf::Shape::wide)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
stem (PO:0009047)
  • decreased thickness (PATO:0000592)
    (Stem::Shape::thin)
  • abnormal (PATO:0000460)
    (Stem::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F19738

Determination of introduced cDNA(s)

  • RAFL04-18-J05 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL04-18-J05 - Resource: pdz87425

    5 prime EST [+] show sequence

    Nucleotide sequence: RAFL04-18-J05 - 5 prime EST 
    TGACTCCCCG TCCTACAGGT CGACTCAGTC TCCGGCTAGT TCGACTCGGT 
    AAAATCACGA TTACTTTCTC CTTCCATTTA TCACGATCGT GGATCCAGAA 
    ACTCTGAAAG ATGGCGTCTG AGCTGACAAA CCGGCGACAC GAGATAGAGC 
    AACCAGAAGC GGAATCTTAT TATCCAAAGC CGATAAAGCC ATGGTTCGTA 
    GCGATTCGTC CGATCCGTTA CATGCTCAGA GAACAGAGAC TCGTCTTCGT 
    CCTCGTCGGC ATTGCCATCG CCACATTAGG ATTCACAATC TTCTCCAAAT 
    CATCCAACCA CCAACCAATT CCTTACGACG TGGATCCGCT TTCCGGATAC 
    GGAATGCGAT CGGAGTCATC GTATCTGCCA GCGACGATTC ATAAGAAGCC 
    AAGCATCGAG TACATGAGCC GGATCGGATC GGCAGGCGGC AAAATCCCTC 
    TAGGCTTGAA ACGGAAAGTG CTAAGGGTTG TTGTGACAGA TGGAGCAGGG 
    TTCGTCGGGT CACACCTGGT GGACCGGTTG ATGGCTCGAG GAGACAACGT 
    GATCGTGGTC GACAATTTCT TCACGGGAAG GAAAGAGAAC GTGATGCACC 
    ATTTCAATAA CCCTAATTTC GAGATGATCC GTCACGATGT AGTGGAGCCG 
    ATCCTTCTAG ANGTTGATCA GATCTACCAT TTGG

    Gene models with high sequence identity

    1. AT2G47650.2 [+] show detail - UDP-xylose synthase 4
      E-value: 0; Score: 99.71

    3 prime EST [+] show sequence

    Nucleotide sequence: RAFL04-18-J05 - 3 prime EST 
    GGCTCAAAAC TACGGTGATT GCATTAAAAA AAATAAAACT TTAAAATCGG 
    TGAGGTTTCA ATATCACTAC ATTGACAGGA ATAGAAATAT CAAAATTAAA 
    AATGAAAACG AAGAGAAACG AGAATCTCCA GCGACAGTTA CTCTGTTGAT 
    GATGAAGTGG TGGAAGAATC CTGCTTTTGG TCGCCAAAGA CACGTTGACG 
    GAAATCTTTG ACCATAAGTG GGAGTCCTTG GCGTAGAGCC ACCTTTGGTT 
    CCCAACCAAG AAGCTCTTTG GCCTTTGTGA TGTCTGGTTT CCTCTTGTGT 
    GGGTCGTCCT CTGTGTTTGG TCTGAACTCT ATCTTTGCGT TCGGGTCAAT 
    TGTCTCTTGA ACCACCTTAG CGAGCTCGAG CATGGTGAAT TCACCGGGGT 
    TACCAAGGTT AAATGGACCG ACATGTTCTC CCTCCATCAA CCTCATCAGA 
    CCTTCCACCA GATCAGAGAC AAACTGGAAA CTCCTAGTCT GCTTACCATC 
    ACCATAAACA GTCAAAGGCT CTTTTCTTAA TGCCTGGGCA ACGAAGTTGC 
    TAACCACACG ACCGTCATCG ATACACATCC TTGGGCCATA AGTGTTGAAT 
    ATCCTAGCAA TCCTAACCTC AACATTAGCG CCTCTGTGAT AGTCCATGGT 
    CAAAGTCTCA GCCGTACGTT TGCCTTCGTC GTAACACCT

    Gene models with high sequence identity

    1. AT2G47650.1 [+] show detail - UDP-xylose synthase 4
      E-value: 0; Score: 100.00

    AT2G47650.1

    Model type
    Protein coding
    Short Description
    UDP-xylose synthase 4
    Curator Summary
    encodes a protein similar to UDP-glucuronic acid decarboxylase. UDP-glucuronic acid decarboxylase produces UDP-xylose, which is a substrate for many cell wall carbohydrates including hemicellulose and pectin. UDP-xylose is also known to feedback regulate several cell wall biosynthetic enzymes.
    Computational Description
    UDP-xylose synthase 4 (UXS4); FUNCTIONS IN: UDP-glucuronate decarboxylase activity, catalytic activity; INVOLVED IN: nucleotide-sugar metabolic process, D-xylose metabolic process; LOCATED IN: Golgi apparatus, plasma membrane, vacuole, membrane; EXPRESSED IN: cotyledon, male gametophyte, cultured cell, pollen tube, leaf; EXPRESSED DURING: L mature pollen stage, M germinated pollen stage; CONTAINS InterPro DOMAIN/s: NAD-dependent epimerase/dehydratase (InterPro:IPR001509), NAD(P)-binding domain (InterPro:IPR016040); BEST Arabidopsis thaliana protein match is: NAD(P)-binding Rossmann-fold superfamily protein (TAIR:AT3G62830.2); Has 44652 Blast hits to 44610 proteins in 2978 species: Archae - 851; Bacteria - 26010; Metazoa - 746; Fungi - 333; Plants - 1297; Viruses - 49; Other Eukaryotes - 15366 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR

    AT2G47650.2

    Model type
    Protein coding
    Short Description
    UDP-xylose synthase 4
    Curator Summary
    encodes a protein similar to UDP-glucuronic acid decarboxylase. UDP-glucuronic acid decarboxylase produces UDP-xylose, which is a substrate for many cell wall carbohydrates including hemicellulose and pectin. UDP-xylose is also known to feedback regulate several cell wall biosynthetic enzymes.
    Computational Description
    UDP-xylose synthase 4 (UXS4); CONTAINS InterPro DOMAIN/s: NAD-dependent epimerase/dehydratase (InterPro:IPR001509), NAD(P)-binding domain (InterPro:IPR016040); BEST Arabidopsis thaliana protein match is: NAD(P)-binding Rossmann-fold superfamily protein (TAIR:AT3G62830.2).
    Link
    InterPro Scan - TAIR