F21211

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F21211

Phenotypes

Morphological phenotypes

Specific phenotypess were NOT observed.

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F21211

Determination of introduced cDNA(s)

  • RAFL06-10-A16 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL06-10-A16 - Resource: pda09376

    full reading [+] show sequence

    Nucleotide sequence: RAFL06-10-A16 - full reading 
    GAGAAGAGGA GGAGAACACA AATCCAATTC ACACTGGAAA CTGTTGGGTC 
    GGATCCTGAG TGATATTTCG AGACCGATGA TGTGGAAGAA CATTGCTGGG 
    TTGTCGAAGG CAGCGGCAGC GGCAAGAACA CACGGATCTC GGCGGTGCTT 
    TTCCACAGCG ATTCCTGGTC CTTGCATCGT CCACAAGCGT GGTGCTGATA 
    TTCTTCACGA TCCATGGTTC AATAAGGACA CAGGTTTTCC TTTGACTGAG 
    AGAGATAGAT TAGGGATACG AGGCTTGCTT CCTCCTCGTG TTATGACCTG 
    TGTACAGCAA TGTGATCGTT TCATTGAGTC GTTTCGTTCG CTAGAGAACA 
    ACACAAAAGG AGAGCCTGAA AACGTTGTTG CATTAGCTAA ATGGAGGATG 
    TTGAATAGAT TGCATGACCG TAATGAGACT TTGTACTACC GAGTTCTTAT 
    TGATAACATC AAAGATTTTG CCCCCATTAT ATACACTCCT ACGGTTGGAC 
    TTGTTTGTCA GAACTATTCT GGTTTGTATA GAAGACCAAG AGGAATGTAC 
    TTCAGCGCCA AAGACAAAGG AGAGATGATG TCTATGATTT ATAACTGGCC 
    TGCTCCGCAG GTAGATATGA TTGTCATCAC TGATGGTAGC CGTATTTTGG 
    GCTTAGGAGA CCTTGGAGTA CAGGGAATTG GGATTCCTAT TGGAAAGCTC 
    GACATGTATG TCGCTGCTGC AGGAATCAAT CCACAGAGAG TTTTGCCAAT 
    TATGTTGGAT GTAGGCACAA ATAATGAAAA GCTCCTACAG AATGATCTCT 
    ATTTAGGAGT CAGACAACCT AGGTTGGAAG GAGAGGAGTA CCTTGAGATT 
    ATTGATGAAT TTATGGAAGC TGCATTTACA CGTTGGCCAA AGGCCGTCGT 
    ACAGTTCGAA GATTTCCAAG CGAAATGGGC TTTCGGAACA TTGGAAAGAT 
    ATCGGAAGAA ATTTTGCATG TTCAATGATG ATGTCCAGGG AACTGCTGGT 
    GTTGCACTGG CTGGACTATT GGGAACTGTA AGAGCTCAAG GTCGGCCGAT 
    ATCCGACTTT GTGAACCAAA AGATTGTTGT GGTGGGAGCT GGAAGTGCTG 
    GGCTTGGTGT AACTAAGATG GCAGTACAGG CAGTTGCCAG AATGGCAGGT 
    ATCAGCGAGA GTGAAGCAAC AAAAAACTTT TACCTGATAG ATAAAGATGG 
    TCTTGTCACC ACGGAGAGGA CAAAACTTGA CCCAGGAGCT GTACTTTTTG 
    CCAAAAATCC AGCTGAGATA CGCGAGGGAG CAAGTATCGT TGAAGTGGTG 
    AAGAAAGTGA GGCCACATGT TCTTCTTGGT TTATCTGGAG TTGGTGGTAT 
    CTTTAATGAA GAAGTTCTCA AGGCGATGCG AGAATCTGAC TCATGCAAGC 
    CTGCCATTTT TGCCATGTCG AATCCCACTT TGAATGCCGA GTGCACTGCT 
    GCTGATGCTT TCAAACACGC CGGAGGAAAC ATTGTCTTTG CAAGTGGAAG 
    TCCATTTGAG AACGTTGAAC TTGAGAATGG TAAAGTAGGA CATGTGAATC 
    AGGCTAACAA CATGTATTTA TTCCCGGGGA TCGGGCTAGG GACTCTTCTC 
    TCTGGTGCAC GTATCGTAAC TGATGGAATG CTACAAGCAG CTTCTGAATG 
    CCTTGCGTCC TACATGACCG ATGAAGAAGT CCAAAAAGGC ATCCTTTACC 
    CTTCAATCAA CAACATCCGA CACATAACAG CTGAAGTCGG GGCAGCTGTT 
    CTACGAGCTG CGGTGACTGA TGACATTGCA GAAGGACATG GTGATGTTGG 
    TCCCAAAGAT CTCAGCCACA TGTCTAAGGA GGATACAGTG AATTACATTA 
    CACGCAACAT GTGGTTCCCT GTTTACAGCC CTCTCGTTCA CGAGAAATAG 
    ACAACAACTC TATATAACTC TTCTCAGGTT TCATAGAAAG CCAAAAGACA 
    AAAAATCTTC GCTTCATCTT TTATTTGTGA TCAACATTCT TTTTTTCTTC 
    AAAATGTGAT ATGTTTTAAG AAAAACAATG CCAAAAAGAG GGCTTAAGAA 
    CAAGAACAGG GTTAGTTCTA GGTCAACCTG TACTTTTCCT TTTTTTTTTT 
    TGGACATTTT TCCAACTTCT TACGATTATT TCTGATGATA ATGATTACAA 
    CTTTATATAC TGTTTTGGCA ATAATAAGCC AATAATGATA TCTTTCTTCT 
    G

    Gene models with high sequence identity

    1. AT4G00570.1 [+] show detail - NAD-dependent malic enzyme 2
      E-value: 0; Score: 99.95

     

    InterPro Scan Digest

    Program Description E-value
    superfamily Aminoacid dehydrogenase-like, N-terminal doma 4e-101
    superfamily NAD(P)-binding domain 1.2e-89
    HMMPanther MALIC ENZYME 1.8e-266
    HMMPanther MALIC ENZYME-RELATED 1.8e-266
    FPrintScan Malic oxidoreductase 5.5e-70
    FPrintScan Malic oxidoreductase 5.5e-70
    FPrintScan Malic oxidoreductase 5.5e-70
    FPrintScan Malic oxidoreductase 5.5e-70
    FPrintScan Malic oxidoreductase 5.5e-70
    FPrintScan Malic oxidoreductase 5.5e-70
    FPrintScan Malic oxidoreductase 5.5e-70
    PatternScan Malic enzyme, conserved site 0
    HMMPfam Malic enzyme, NAD-binding 1.4e-84
    HMMPfam Malic enzyme, N-terminal 8.2e-75
    Gene3D NAD(P)-binding domain 1.9e-74

    See the detailed result >

    AT4G00570.1

    Model type
    Protein coding
    Short Description
    NAD-dependent malic enzyme 2
    Curator Summary
    Encodes an NAD-dependent malic enzyme (NAD-ME) that does not act on oxaloacetate, indicating that it belongs to EC 1.1.1.39. It is a member of the beta family of NAD-MEs in plants. It appears to function as a homodimer or as a heterodimer with the alpha-type NAD-ME2 (At2g13560). NAD-ME2 transcript and protein levels are higher during the night than during the day.
    Computational Description
    NAD-dependent malic enzyme 2 (NAD-ME2); FUNCTIONS IN: in 7 functions; INVOLVED IN: malate metabolic process; LOCATED IN: mitochondrion, chloroplast; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; CONTAINS InterPro DOMAIN/s: Malic enzyme, NAD-binding (InterPro:IPR012302), Malic oxidoreductase (InterPro:IPR001891), Malic enzyme, conserved site (InterPro:IPR015884), Malic enzyme, N-terminal (InterPro:IPR012301), NAD(P)-binding domain (InterPro:IPR016040); BEST Arabidopsis thaliana protein match is: NAD-dependent malic enzyme 1 (TAIR:AT2G13560.1); Has 9343 Blast hits to 9326 proteins in 2439 species: Archae - 143; Bacteria - 6323; Metazoa - 607; Fungi - 223; Plants - 463; Viruses - 0; Other Eukaryotes - 1584 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR