F22207

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F22207

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage
Phenotypes
T1  
rosette leaf (PO:0000014)
  • dark green (PATO:0001249)
    (Rosette leaf::Color::dark)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
fruit (PO:0009001)
  • abnormal (PATO:0000460)
    (Silique::Others)
flower (PO:0009046)
  • abnormal (PATO:0000460)
    (Flower::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F22207

Determination of introduced cDNA(s)

  • RAFL09-83-E23 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL09-83-E23 - Resource: pda09951

    full reading [+] show sequence

    Nucleotide sequence: RAFL09-83-E23 - full reading
    ATTTTTATTT GAAAGACATT TTTGCCTATT TCATTCATAT TTTGCTTTTA 
    AAACGAGAAT TATAATTCTT CGACTCCGTG GGATAAGTTT TCTAAAAAAT
    CGAAGAAAGA TCGATGGCTC TAGATATCTG CGTGAAGGTT GCCGTCGGTG
    CTCCTGATGT TCTCGGCGAC TGTCCGTTTA GCCAACGTGT TCTTCTGACA
    CTTGAGGAGA AGAAGCTTCC CTACAAGACC CATCTGATTA ACGTCTCCGA
    CAAACCCCAA TGGTTCTTAG ACATTAGTCC AGAGGGGAAA GTTCCGGTGG
    TGAAGCTTGA TGGCAAATGG GTGGCTGATT CTGACGTAAT TGTTGGACTT
    CTTGAAGAGA AATATCCAGA GCCTTCTCTC AAGACTCCTC CTGAATTTGC
    TTCTGTAGGA TCCAAAATCT TTGGTGCTTT TGTGACTTTC TTGAAGAGCA
    AAGACGCTAA TGACGGATCC GAGAAGGCTT TGGTTGATGA GTTAGAAGCG
    TTGGAGAATC ACTTGAAGAC ACATTCTGGT CCTTTTGTAG CTGGAGAGAA
    GATTACTGCA GTGGATTTGA GTTTAGCACC AAAGCTTTAC CATCTTGAGG
    TTGCTCTTGG TCATTACAAG AACTGGTCTG TCCCTGAGAG CTTGACCAGT
    GTTCGTAACT ACGCCAAGGC TTTGTTCTCT AGGGAGTCGT TTGAGAACAC
    CAAGGCTAAG AAAGAGATTG TGGTTGCGGG TTGGGAATCG AAGGTGAATG
    CGTGATGTGG ATCCATGCTT CTCTAGTTAA TGTTGCTTTA GAACTATTCT
    TCACTGAATA ATAAGAATGC ACGAGTATGT GTGTGTTCTT GTTTTTGTTA
    GAGTTGGTTA TCAATAATGA TGCGATAAAG CAGCATATCT TTTTTTGATA
    ATATACACCT CTTATTCAAT TTGGTATT

    Gene models with high sequence identity

    1. AT1G75270.1 [+] show detail - dehydroascorbate reductase 2
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    HMMPfam Glutathione S-transferase, N-terminal 0.0000007
    HMMPanther DEHYDROASCORBATE REDUCTASE 2e-141
    HMMPanther GLUTATHIONE S-TRANSFERASE, GST, SUPERFAMILY, 2e-141
    ProfileScan Glutathione S-transferase, N-terminal 14.488
    ProfileScan Glutathione S-transferase/chloride channel, C-terminal 11.051
    Gene3D Thioredoxin fold 3.4e-19
    Gene3D Glutathione S-transferase, C-terminal-like 4.1e-39
    superfamily Glutathione S-transferase, C-terminal-like 1e-25
    superfamily Thioredoxin-like fold 4e-20

    See the detailed result >

    AT1G75270.1

    Model type
    Protein coding
    Short Description
    dehydroascorbate reductase 2
    Computational Description
    dehydroascorbate reductase 2 (DHAR2); FUNCTIONS IN: glutathione binding, glutathione dehydrogenase (ascorbate) activity; INVOLVED IN: protein amino acid glutathionylation; LOCATED IN: plasma membrane; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: Thioredoxin fold (InterPro:IPR012335), Glutathione S-transferase, C-terminal-like (InterPro:IPR010987), Glutathione S-transferase/chloride channel, C-terminal (InterPro:IPR017933), Glutathione S-transferase, N-terminal (InterPro:IPR004045), Thioredoxin-like fold (InterPro:IPR012336); BEST Arabidopsis thaliana protein match is: dehydroascorbate reductase (TAIR:AT1G19570.1); Has 4940 Blast hits to 4851 proteins in 1062 species: Archae - 0; Bacteria - 1874; Metazoa - 1090; Fungi - 227; Plants - 921; Viruses - 0; Other Eukaryotes - 828 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR