F22620

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F22620

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • increased size (PATO:0000586)
    (Seed::Shape::large)
  • increased length (PATO:0000573)
    (Seed::Shape::long)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F22620

Determination of introduced cDNA(s)

  • RAFL08-12-L24 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL08-12-L24 - Resource: pda04739

    full reading [+] show sequence

    Nucleotide sequence: RAFL08-12-L24 - full reading 
    GGTTTTGGCA CATTACCCTC TTTCCTGTTA AAGTTGTCTC GTCTCCTTCT 
    CGTCTCCTTC CTCAAGCATA TTCTCTGCGC TTAAAGTACT TTGATGGCAT 
    TGGGACACTT GTGCAGCATC CACTGTTTCC TTAAAATAGG GAAACAAGAA 
    GAGCTTCTTG TCCTAGATAT TGGTTTGCCT ATTCCCAGTT TCTTCCTCAT 
    TAACTTTATT GGTAGCAGAG CCTCATCCAT CTGGTGAACT TGTGAAGCTT 
    CCTCTGTGAT TCTTTTGGCG ATTGGTTAGA AATATGGTTT CTTCTCAGTG 
    TTCTGTTGCA AACAAAAACC AGACTGGGAA GCCGTTTCAG AAACATCTAT 
    CTTTATCCAT CAGCCCTCCA AAATCTGTCT TGGGTGATAA CCTTGAGCTT 
    CAATTCTCTG ATGTCTTTGG ACCAATGCCT GAAGCTAACT CTGAAGAGGC 
    CTGTGATGTT GCTTACGACG AGCCTGCTGT TGTCTACAGT CGATCCCACT 
    CATTGGTTGG CCCGTCTTTG GTTGTTAGCC ATTCTTTGAA GATGAACAAG 
    CTCACTTTAC GAGAAACTGA GGACTCAGTT GACTTGGTGG AGTGTGTTGA 
    AGGTGAATCG ATAAAAGAGA ACGATGAATT CTCTGGTAAT GATGACACTG 
    ACAGTGAGAA ATCTCCAGAG GAGGTCTCAG GTGTAGTAGG CATTGAGGAC 
    TTTGAGGTAT TGAAGGTTGT GGGACAAGGT GCATTTGGAA AAGTGTACCA 
    GGTGAGGAAA AAAGACACGT CTGAGATATA CGCGATGAAG GTCATGAGAA 
    AAGATAAAAT TGTTGAGAAG AATCATGCTG AATACATGAA AGCCGAGCGC 
    GATATTCTAA CCAAAATCGA TCATCCTTTC ATTGTGCAAC TTAAATACTC 
    TTTTCAGACC AAATACAGAT TGTATCTTGT TCTTGACTTT ATAAACGGAG 
    GTCATCTTTT CTTCCAGCTC TATCACCAAG GGCTTTTCAG GGAGGACTTG 
    GCTCGTGTGT ACACTGCAGA AATCGTCTCT GCAGTTTCCC ATCTCCATGA 
    GAAAGGCATA ATGCATAGAG ATCTTAAACC TGAAAACATA CTCATGGACG 
    TAGATGGCCA TGTGATGTTA ACTGATTTTG GTTTAGCAAA AGAATTTGAA 
    GAAAACACAA GATCAAACTC CATGTGTGGG ACTACGGAGT ATATGGCCCC 
    TGAGATTGTT CGTGGAAAAG GACATGATAA GGCTGCCGAC TGGTGGAGTG 
    TAGGAATCCT TCTGTATGAG ATGCTCACTG GAAAGCCGCC GTTTCTCGGG 
    AGCAAAGGAA AGATACAGCA GAAAATCGTT AAGGACAAGA TCAAGCTTCC 
    ACAGTTTCTG TCTAATGAAG CTCATGCCTT GCTGAAAGGG CTGCTGCAAA 
    AAGAGCCAGA AAGGAGACTG GGAAGTGGAC CGAGCGGAGC AGAGGAGATA 
    AAAAAAACAC AAATGGTTCA AGGCGATAAA CTGGAAGAAG CTGGAGGCTA 
    GAGAAGTACA ACCAAGTTTC AAGCCGGCGG TTTCGGGAAG ACAATGCATA 
    GCTAATTTTG ACAAGTGTTG GACTGACATG TCTGTTTTGG ATTCTCCAGC 
    AAGTAGTCCC AACTCTGATG CTAAGGCGAA CCCTTTTACA AACTTCACAT 
    ACGTCAGGCC TCCTCATTCA TTCCTTCATC GGACCACATC CAACTTGTAG 
    ATGAACTATA ATATGTTACC CCCGCTTCTG TTTTTGTAAT CCGAAACATA 
    TGAACATGCA CAAAATCAAC GGTTGTTTGA AGAAACCTAT TTCTCGATTT 
    AATATATTAT ACTAGACAAA AAAAAAAAAA AAA

    Gene models with high sequence identity

    1. AT3G08720.1 [+] show detail - serine/threonine protein kinase 2
      E-value: 0; Score: 99.94

     

    InterPro Scan Digest

    Program Description E-value
    PatternScan Protein kinase, ATP binding site 0
    PatternScan Serine/threonine-protein kinase, active site 0
    HMMPfam Serine/threonine-protein kinase-like domain 1.6e-72
    HMMPfam Protein kinase, C-terminal 2e-10
    superfamily Protein kinase-like domain 8.3e-89
    Gene3D no description 2.4e-65
    HMMSmart Serine/threonine-protein kinase domain 9.8e-104
    HMMSmart Tyrosine-protein kinase, catalytic domain 3.1e-11
    HMMSmart AGC-kinase, C-terminal 1.4e-16
    ProfileScan Protein kinase, catalytic domain 51.707
    ProfileScan AGC-kinase, C-terminal 13.108
    HMMPanther RIBOSOMAL PROTEIN S6 KINASE BETA 5.1e-161
    HMMPanther SERINE/THREONINE PROTEIN KINASE 5.1e-161
    HMMPanther RIBOSOMAL PROTEIN S6 KINASE BETA 1.8e-23
    HMMPanther SERINE/THREONINE PROTEIN KINASE 1.8e-23

    See the detailed result >

    AT3G08720.1

    Model type
    Protein coding
    Short Description
    serine/threonine protein kinase 2
    Curator Summary
    Encodes a ribosomal-protein S6 kinase. Gene expression is induced by cold and salt (NaCl). Activation of AtS6k is regulated by 1-naphthylacetic acid and kinetin, at least in part, via a lipid kinase-dependent pathway. Phosphorylates specifically mammalian and plant S6 at 25 degrees C but not at 37 degrees C. Involved in translational up-regulation of ribosomal proteins.
    Computational Description
    serine/threonine protein kinase 2 (S6K2); CONTAINS InterPro DOMAIN/s: Protein kinase, ATP binding site (InterPro:IPR017441), Serine/threonine-protein kinase domain (InterPro:IPR002290), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), AGC-kinase, C-terminal (InterPro:IPR000961), Protein kinase, C-terminal (InterPro:IPR017892), Serine/threonine-protein kinase, active site (InterPro:IPR008271), Protein kinase-like domain (InterPro:IPR011009), Protein kinase, catalytic domain (InterPro:IPR000719), Tyrosine-protein kinase, catalytic domain (InterPro:IPR020635); BEST Arabidopsis thaliana protein match is: protein-serine kinase 1 (TAIR:AT3G08730.1); Has 133901 Blast hits to 131707 proteins in 4554 species: Archae - 204; Bacteria - 15943; Metazoa - 48718; Fungi - 13429; Plants - 32816; Viruses - 561; Other Eukaryotes - 22230 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR