F22806

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F22806

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
fruit (PO:0009001)
  • abnormal (PATO:0000460)
    (Silique::Other)
stem (PO:0009047)
  • abnormal (PATO:0000460)
    (Stem::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F22806

Determination of introduced cDNA(s)

  • RAFL06-16-M11 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL06-16-M11 - Resource: pdz87619

    5 prime EST [+] show sequence

    Nucleotide sequence: RAFL06-16-M11 - 5 prime EST 
    GGTCTCGTTG TCCTCCTCAC TTTCATCAGC CGTTTTGAAT CTCCGGCGAC 
    TTGACAGAGA AGAACAAGGA AGAAGACTAA GAGAGAAAGT AAGAGATAAT 
    CCAGGAGATT CATTCTCCGT TTTGAATCTT CCTCAATCTC ATCTTCTTCC 
    GCTCTTTCTT TCCAAGCTCA TAAAAAATGG CTGAGGCTGA TGATATTCAA 
    CCAATCGTGT GTGACAATGG TACCGGTATG GTGAAGGCTG GATTTGCAGG 
    AGATGATGCT CCCAGGGCTG TTTTTCCCAG TGTTGTTGGT AGGCCAAGAC 
    ATCATGGTGT CATGGTTGGG ATGAACCAGA AGGATGCATA TGTTGGTGAT 
    GAAGCACAAT CCAAGAGAGG TATTCTTACC TTGAAGTATC CTATTGAGCA 
    TGGTGTTGTT AGCAACTGGG ATGATATGGA AAAGATCTGG CATCACACTT 
    TCTACAATGA GCTTCGTATT GCTCCTGAAG AGCACCCTGT TCTTCTTACC 
    GAGGCTCCTC TTAACCCAAA GGCCAACAGA GAGAAGATGA CTCAAATCAT 
    GTTTGAGACC TTTTAACTCT CCCGCTATGT ATGTCNCCAT CCAAGCTGTT 
    CTCTTCTTGT ACGCCAGCGG CCGTACAACC GGTATTGTGC TGGATTCTGG 
    TGANGGTGCG TCTCNCC

    Gene models with high sequence identity

    1. AT3G18780.2 [+] show detail - actin 2
      E-value: 0; Score: 98.94

    3 prime EST [+] show sequence

    Nucleotide sequence: RAFL06-16-M11 - 3 prime EST 
    CGGAATAAAG AGGCATCAAT TCGATCACTC AGAGCTACAA AACAATGGGA 
    CTAAAACGCA AAACGAAAGC GGTTTTTTTT TTTGAAACGC AGACGTAAGT 
    AAAAACCCAG AGAGTTTGTC ACACACAAGT GCATCATAGA AACGAAAACA 
    AAAAGGGAAA TGAAACAAAC AAATGGAGAA GCAAATATAA AAGAAAAAAA 
    AGAAACTTTG ATCCCATTCA TAAAACCCCA GCTTTTTAAG CCTTTGATCT 
    TGAGAGCTTA

    Gene models with high sequence identity

    1. AT3G18780.2 [+] show detail - actin 2
      E-value: 2e-140; Score: 99.61

    AT3G18780.2

    Model type
    Protein coding
    Short Description
    actin 2
    Curator Summary
    Encodes an actin that is constitutively expressed in vegetative structures but not pollen. ACT2 is involved in tip growth of root hairs.
    Computational Description
    actin 2 (ACT2); FUNCTIONS IN: structural constituent of cytoskeleton; INVOLVED IN: in 7 processes; LOCATED IN: nucleus, membrane, cytoplasm; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 16 growth stages; CONTAINS InterPro DOMAIN/s: Actin, conserved site (InterPro:IPR004001), Actin/actin-like (InterPro:IPR004000), Actin/actin-like conserved site (InterPro:IPR020902); BEST Arabidopsis thaliana protein match is: actin 8 (TAIR:AT1G49240.1); Has 15142 Blast hits to 14749 proteins in 3039 species: Archae - 6; Bacteria - 12; Metazoa - 5705; Fungi - 5217; Plants - 1599; Viruses - 2; Other Eukaryotes - 2601 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR