F23032

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F23032

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T1  
rosette leaf (PO:0000014)
  • abnormal (PATO:0000460)
    (Rosette leaf::Others)
fruit (PO:0009001)
  • decreased length (PATO:0000574)
    (Silique::Shape::short)
stem (PO:0009047)
  • abnormal (PATO:0000460)
    (Stem::Others)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F23032

Determination of introduced cDNA(s)

  • RAFL05-03-G03 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-03-G03 - Resource: pda00687

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-03-G03 - full reading 
    TGCTCTCTCG ATTGACTTTA TTGACTGAGA GAAAGCTTTT GACAATGGGC 
    CACAGACACA GCAAGTCCAA ATCCTCCGAT CCGCCACCGT CCTCCTCCTC 
    CTCTTCCTCC GGCAACGTAG TCCACCATGT TAAACCTGCC GGAGAACGCC 
    GCGGATCATC CGGTTCCGGA ACCGTCGGAT CGTCAGGCTC CGGAACAGGC 
    GGATCTCGTT CCACCACATC TACTCAGCAG AATGGTCGGA TCTTAGGTCG 
    GCCGATGGAG GAAGTACGGA GAACCTATGA GTTTGGTCGT GAGCTTGGTC 
    GTGGCCAGTT TGGTGTCACG TACCTCGTAA CTCACAAAGA GACGAAACAA 
    CAGGTCGCTT GCAAGTCAAT CCCTACGCGC CGCCTTGTTC ACAAAGACGA 
    CATTGAAGAT GTTCGCCGTG AAGTCCAGAT TATGCACCAT CTCAGCGGTC 
    ACCGGAACAT AGTGGACTTG AAGGGAGCCT ACGAGGACAG ACACTCTGTG 
    AATCTGATAA TGGAGTTGTG TGAAGGAGGG GAATTGTTCG ATAGGATCAT 
    TTCTAAAGGT CTTTACTCAG AGAGAGCTGC TGCGGATTTG TGTAGGCAGA 
    TGGTGATGGT TGTGCATAGT TGTCATTCTA TGGGTGTAAT GCACCGAGAC 
    TTGAAGCCCG AAAACTTTCT CTTTCTTAGT AAAGATGAGA ACTCACCATT 
    GAAAGCTACA GACTTTGGTC TCTCTGTCTT CTTCAAGCCA GGTGATAAGT 
    TTAAGGATCT TGTTGGAAGT GCATACTATG TTGCCCCAGA AGTTCTAAAA 
    CGGAACTATG GACCAGAGGC TGATATCTGG AGTGCTGGTG TGATTCTATA 
    CATCCTTCTC AGTGGTGTTC CGCCTTTCTG GGGAGAAAAT GAAACGGGGA 
    TCTTTGATGC CATTCTTCAA GGGCAACTTG ATTTTTCAGC TGATCCATGG 
    CCAGCACTAT CAGATGGTGC CAAAGATCTT GTGAGGAAAA TGTTAAAATA 
    TGACCCTAAA GATCGGCTTA CAGCTGCGGA AGTGCTAAAT CATCCGTGGA 
    TTAGAGAAGA TGGGGAGGCA TCAGACAAAC CGCTTGACAA TGCGGTGTTA 
    TCCAGGATGA AACAATTCCG GGCGATGAAC AAACTAAAAA AAATGGCCCT 
    GAAGGTTATC GCTGAGAACC TTTCTGAAGA AGAAATCATT GGTCTTAAAG 
    AGATGTTCAA ATCTCTAGAC ACTGATAACA ATGGAATAGT CACCTTGGAG 
    GAATTGAGAA CCGGTCTCCC AAAACTTGGT TCAAAGATCT CTGAAGCTGA 
    AATAAGGCAG TTAATGGAAG CAGCTGATAT GGATGGAGAT GGATCAATTG 
    ATTACTTGGA GTTTATATCA GCCACAATGC ATATGAACAG AATCGAACGT 
    GAGGATCACT TGTACACCGC ATTCCAGTTC TTTGACAATG ACAACAGCGG 
    CTACATAACA ATGGAAGAGC TAGAGCTAGC CATGAAGAAA TACAACATGG 
    GTGATGATAA ATCCATCAAA GAGATCATTG CTGAAGTAGA CACCGATCGT 
    GACGGAAAAA TCAACTACGA AGAATTTGTA GCGATGATGA AGAAGGGAAA 
    TCCAGAATTG GTGCCAAACC GACGCAGAAT GTGAGAGATC TTTTGATGTT 
    GTTTGTTTGT TTTTGGTTAT CACTTTACTC AAACTTTGTG TTTCTGTTAC 
    AATCAATTAT TTTGTTTCCT GC

    Gene models with high sequence identity

    1. AT4G23650.1 [+] show detail - calcium-dependent protein kinase 6
      E-value: 0; Score: 100.00

     

    InterPro Scan Digest

    Program Description E-value
    ProfileScan Protein kinase, catalytic domain 48.612
    ProfileScan EF-HAND 2 13.9
    ProfileScan EF-HAND 2 11.64
    ProfileScan EF-HAND 2 14.513
    ProfileScan EF-HAND 2 10.915
    superfamily Protein kinase-like domain 1.2e-91
    superfamily EF-hand 2.3e-70
    HMMPanther Calcium-dependent protein kinase 1.5e-273
    HMMPanther Calcium/calmodulin-dependent protein kinase-like 1.5e-273
    HMMSmart Serine/threonine-protein kinase domain 1.7e-102
    HMMSmart Tyrosine-protein kinase, catalytic domain 3.9e-11
    HMMSmart Calcium-binding EF-hand 0.00011
    HMMSmart Calcium-binding EF-hand 0.0075
    HMMSmart Calcium-binding EF-hand 0.081
    HMMSmart Calcium-binding EF-hand 1.6e-07
    Gene3D no description 1.3e-24
    Gene3D no description 2.9e-60
    Gene3D EF-hand-like domain 1.3e-39
    PatternScan EF-Hand 1, calcium-binding site 0
    PatternScan EF-Hand 1, calcium-binding site 0
    PatternScan EF-Hand 1, calcium-binding site 0
    PatternScan EF-Hand 1, calcium-binding site 0
    PatternScan Protein kinase, ATP binding site 0
    PatternScan Serine/threonine-protein kinase, active site 0
    HMMPfam Serine/threonine-protein kinase-like domain 5.7e-71
    HMMPfam EF-hand 3.6e-06
    HMMPfam EF-hand 2e-09

    See the detailed result >

    AT4G23650.1

    Model type
    Protein coding
    Short Description
    calcium-dependent protein kinase 6
    Curator Summary
    Encodes calcium dependent protein kinase 3 (CPK3), a member of the Arabidopsis CDPK gene family. CDPKs contain an intrinsic Ca2+-activation domain with four EF hand Ca2+-binding sites. CDPKs protein kinases have been proposed to function in multiple plant signal transduction pathways downstream of [Ca2+]cyt elevations, thus transducing various physiological responses. CPK3 is expressed in both guard cells and mesophyll cells. Functions in guard cell ion channel regulation. ABA and Ca(2+) activation of slow-type anion channels and, interestingly, ABA activation of plasma membrane Ca(2+)-permeable channels were impaired in independent alleles of single and double cpk3cpk6 mutant guard cells. Furthermore, ABA- and Ca(2+)-induced stomatal closing were partially impaired in these cpk3cpk6 mutant alleles. CPK6 is also a member of the Arabidopsis CDPK family.
    Computational Description
    calcium-dependent protein kinase 6 (CDPK6); FUNCTIONS IN: calmodulin-dependent protein kinase activity, protein kinase activity, kinase activity; INVOLVED IN: in 6 processes; LOCATED IN: plasma membrane, vacuole, membrane; EXPRESSED IN: 26 plant structures; EXPRESSED DURING: 13 growth stages; CONTAINS InterPro DOMAIN/s: Protein kinase, ATP binding site (InterPro:IPR017441), EF-Hand 1, calcium-binding site (InterPro:IPR018247), Serine/threonine-protein kinase domain (InterPro:IPR002290), Calcium-binding EF-hand (InterPro:IPR002048), EF-hand-like domain (InterPro:IPR011992), EF-hand (InterPro:IPR018248), Serine/threonine-protein kinase-like domain (InterPro:IPR017442), Protein kinase-like domain (InterPro:IPR011009), Serine/threonine-protein kinase, active site (InterPro:IPR008271), Protein kinase, catalytic domain (InterPro:IPR000719), EF-HAND 2 (InterPro:IPR018249), Calcium-dependent protein kinase (InterPro:IPR020642), Calcium/calmodulin-dependent protein kinase-like (InterPro:IPR020636); BEST Arabidopsis thaliana protein match is: calcium-dependent protein kinase 17 (TAIR:AT5G12180.1); Has 137650 Blast hits to 128872 proteins in 4025 species: Archae - 155; Bacteria - 13983; Metazoa - 52031; Fungi - 17582; Plants - 29890; Viruses - 500; Other Eukaryotes - 23509 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR