F23034

RIKEN Arabidopsis full-length cDNA overexpressed Arabidopsis lines

Original: F23034

Phenotypes

Morphological phenotypes

Generation Anatomical entity
/structure development stage		 Phenotypes
T2 seed (PO:0009010)
  • increased size (PATO:0000586)
    (Seed::Shape::large)
  • increased length (PATO:0000573)
    (Seed::Shape::long)

Invisible phenotypes

Specific phenotypes were NOT observed.

Original: F23034

Determination of introduced cDNA(s)

  • RAFL05-21-M07 - RIKEN Arabidopsis Full-length cDNAs - Original: RAFL05-21-M07 - Resource: pda17147

    full reading [+] show sequence

    Nucleotide sequence: RAFL05-21-M07 - full reading 
    GAATTGCAAA TCAAATGGAT CGTCTTAAGC TTTATTTCTC CGTTTTCGTT 
    TTGTCTTTCT TTATCGTCTC GGTTTCGTCG TCTGATGTCA ACGACGGCGA 
    TGATCTCGTG ATCCGTCAGG TGGTTGGTGG AGCCGAGCCT CAGGTTTTGA 
    CCTCAGAGGA TCACTTTTCT CTCTTCAAGC GGAAGTTCGG GAAGGTCTAC 
    GCTTCCAACG AGGAGCATGA CTATAGATTC TCGGTTTTCA AAGCGAATCT 
    CAGGCGAGCG AGGCGTCACC AGAAGTTGGA TCCGTCGGCG ACTCATGGTG 
    TTACGCAGTT CTCAGATCTG ACTCGGTCTG AGTTCCGTAA GAAGCACTTG 
    GGGGTTAGAA GTGGCTTTAA GCTTCCTAAA GATGCCAACA AGGCTCCGAT 
    TCTCCCTACC GAAAATCTCC CTGAGGATTT TGATTGGAGA GATCATGGCG 
    CCGTTACTCC CGTCAAAAAT CAGGGATCTT GCGGCTCTTG CTGGAGTTTC 
    AGCGCCACTG GAGCTTTGGA AGGTGCTAAC TTCCTCGCTA CCGGCAAACT 
    CGTCAGCCTC AGCGAACAAC AGCTCGTCGA CTGTGATCAC GAGTGTGATC 
    CCGAGGAGGC AGATTCCTGC GACTCTGGTT GCAATGGTGG GCTAATGAAC 
    AGCGCTTTTG AATACACCCT CAAAACCGGA GGGCTCATGA AAGAAGAAGA 
    CTATCCTTAC ACCGGAAAGG ACGGCAAGAC CTGCAAGCTA GACAAGTCCA 
    AGATCGTTGC CTCTGTCTCC AACTTCAGTG TTATCTCCAT TGATGAAGAA 
    CAGATTGCTG CAAACCTTGT CAAGAACGGA CCTCTTGCTG TAGCCATCAA 
    CGCTGGCTAT ATGCAGACTT ACATTGGAGG AGTCTCATGC CCTTACATAT 
    GCACCAGGAG GCTCAACCAC GGTGTCTTAT TGGTTGGCTA TGGAGCGGCA 
    GGTTACGCTC CGGCTAGGTT CAAGGAGAAG CCTTACTGGA TCATCAAGAA 
    CTCGTGGGGA GAGACTTGGG GTGAAAATGG TTTCTACAAA ATCTGCAAAG 
    GCCGTAACAT TTGTGGTGTT GACAGTATGG TCTCCACTGT TGCAGCCACC 
    GTCTCAACCA CCGCCCATTA AGCATCTCGT CAATAAGTTT TAATTACTTT 
    GGTGATTTGT ATGAGCGAGC TCTCTTTGCG CTGCTGACTC TCTCTATTTA 
    TCTCTGCTTC TTGCTTGTAA ATAAAATGCG TTCTATTGAC CAAAAAAAAA 
    A

    Gene models with high sequence identity

    1. AT4G39090.1 [+] show detail - Papain family cysteine protease
      E-value: 0; Score: 99.92

     

    InterPro Scan Digest

    Program Description E-value
    Gene3D no description 3.6e-98
    HMMSmart Proteinase inhibitor I29, cathepsin propeptide 7.1e-23
    HMMSmart Peptidase C1A, papain C-terminal 1.8e-111
    superfamily Cysteine proteinases 1.8e-104
    PatternScan Peptidase, cysteine peptidase active site 0
    PatternScan Peptidase, cysteine peptidase active site 0
    PatternScan Peptidase, cysteine peptidase active site 0
    HMMPfam Peptidase C1A, papain C-terminal 2.8e-75
    HMMPfam Proteinase inhibitor I29, cathepsin propeptide 1.9e-12
    HMMPanther CYSTEINE PROTEASE 6.4e-238
    HMMPanther Peptidase C1A, papain 6.4e-238
    FPrintScan Peptidase C1A, papain C-terminal 1.6e-10
    FPrintScan Peptidase C1A, papain C-terminal 1.6e-10
    FPrintScan Peptidase C1A, papain C-terminal 1.6e-10

    See the detailed result >

    AT4G39090.1

    Model type
    Protein coding
    Short Description
    Papain family cysteine protease
    Curator Summary
    Similar to cysteine proteinases, induced by desiccation but not abscisic acid. Required for RRS1-R mediated resistance against Ralstonia solanacearum. Interacts with the R. solanacearum type III effector PopP2. RD19 associates with PopP2 to form a nuclear complex that is required for activation of the RRS1-R
    Computational Description
    RESPONSIVE TO DEHYDRATION 19 (RD19); CONTAINS InterPro DOMAIN/s: Peptidase C1A, papain (InterPro:IPR013128), Proteinase inhibitor I29, cathepsin propeptide (InterPro:IPR013201), Peptidase C1A, papain C-terminal (InterPro:IPR000668), Peptidase, cysteine peptidase active site (InterPro:IPR000169); BEST Arabidopsis thaliana protein match is: Papain family cysteine protease (TAIR:AT2G21430.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink).
    Link
    InterPro Scan - TAIR